LOCUS       sequence-322777-        7343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector expressing the TnsA, TnsB, and TnsC transposition proteins 
            from the Tn6900 derivative element with a lactose promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Petassi MT, Hsieh SC, Peters JE
  TITLE     Guide RNA Categorization Enables Target Site Choice in 
            Tn7-CRISPR-Cas Transposons.
  JOURNAL   Cell. 2020 Nov 30. pii: S0092-8674(20)31464-1. doi: 
            10.1016/j.cell.2020.11.005.
  PUBMED    33271061
REFERENCE   2  (bases 1 to 7343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Nov 30. pii: S0092-8674(20)31464-1. doi: 
            10.1016/j.cell.2020.11.005."
COMMENT     Sequence Label: pMTP130 (pTA106_TnsABC(Tn6900))
FEATURES             Location/Qualifiers
     source          1..7343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(190..258,259..1050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(408..427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1656..1878
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1926..2876
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     protein_bind    3701..3722
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3737..3742,3743..3760,3761..3767)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3775..3791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(join(7342..7343,1..17))
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
       61 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      121 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      181 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      241 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      301 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      361 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      421 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      481 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
      541 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
      601 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
      661 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
      721 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
      781 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
      841 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
      901 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
      961 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1021 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1081 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1141 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgtt
     1201 gatgataccg ctgccttact gggtgcatta gccagtctga atgacctgtc acgggataat
     1261 ccgaagtggt cagactggaa aatcagaggg caggaactgc tgaacagcaa aaagtcagat
     1321 agcaccacat agcagacccg ccataaaacg ccctgagaag cccgtgacgg gcttttcttg
     1381 tattatgggt agtttccttg catgaatcca taaaaggcgc ctgtagtgcc atttaccccc
     1441 attcactgcc agagccgtga gcgcagcgaa ctgaatgtca cgaaaaagac agcgactcag
     1501 gtgcctgatg gtcggagaca aaaggaatat tcagcgattt gcccgagctt gcgagggtgc
     1561 tacttaagcc tttagggttt taaggtctgt tttgtagagg agcaaacagc gtttgcgaca
     1621 tccttttgta atactgcgga actgactaaa gtagtgagtt atacacaggg ctgggatcta
     1681 ttctttttat ctttttttat tctttcttta ttctataaat tataaccact tgaatataaa
     1741 caaaaaaaac acacaaaggt ctagcggaat ttacagaggg tctagcagaa tttacaagtt
     1801 ttccagcaaa ggtctagcag aatttacaga tacccacaac tcaaaggaaa aggactagta
     1861 attatcattg actagcccat ctcaattggt atagtgatta aaatcaccta gaccaattga
     1921 gatgtatgtc tgaattagtt gttttcaaag caaatgaact agcgattagt cgctatgact
     1981 taacggagca tgaaaccaag ctaattttat gctgtgtggc actactcaac cccacgattg
     2041 aaaaccctac aaggaaagaa cggacggtat cgttcactta taaccaatac gctcagatga
     2101 tgaacatcag tagggaaaat gcttatggtg tattagctaa agcaaccaga gagctgatga
     2161 cgagaactgt ggaaatcagg aatcctttgg ttaaaggctt tgagattttc cagtggacaa
     2221 actatgccaa gttctcaagc gaaaaattag aattagtttt tagtgaagag atattgcctt
     2281 atcttttcca gttaaaaaaa ttcataaaat ataatctgga acatgttaag tcttttgaaa
     2341 acaaatactc tatgaggatt tatgagtggt tattaaaaga actaacacaa aagaaaactc
     2401 acaaggcaaa tatagagatt agccttgatg aatttaagtt catgttaatg cttgaaaata
     2461 actaccatga gtttaaaagg cttaaccaat gggttttgaa accaataagt aaagatttaa
     2521 acacttacag caatatgaaa ttggtggttg ataagcgagg ccgcccgact gatacgttga
     2581 ttttccaagt tgaactagat agacaaatgg atctcgtaac cgaacttgag aacaaccaga
     2641 taaaaatgaa tggtgacaaa ataccaacaa ccattacatc agattcctac ctacataacg
     2701 gactaagaaa aacactacac gatgctttaa ctgcaaaaat tcagctcacc agttttgagg
     2761 caaaattttt gagtgacatg caaagtaagt atgatctcaa tggttcgttc tcatggctca
     2821 cgcaaaaaca acgaaccaca ctagagaaca tactggctaa atacggaagg atctgaggtt
     2881 cttatggctc ttgtatctat cagtgaagca tcaagactaa caaacaaaag tagaacaact
     2941 gttcaccgtt acatatcaaa gggaaaactg tccatatgca cagatgaaaa cggtgtaaaa
     3001 aagatagata catcagagct tttacgagtt tttggtgcat tcaaagctgt tcaccatgaa
     3061 cagatcgaca atgtaacaga tgaacagcat gtaacaccta atagaacagg tgaaaccagt
     3121 aaaacaaagc aactagaaca tgaaattgaa cacctgagac aacttgttac agctcaacag
     3181 tcacacatag acagcctgaa acaggcgatg ctgcttatcg aatcaaagct gccgacaaca
     3241 cgggagccag tgacgcctcc cgtggggaaa aaatcatggc aattctggaa gaaatagcgc
     3301 tcaaagatgc aggggtaaaa gctaaccgca tctttaccga caaggcatcc ggcagttcaa
     3361 cagatcggga agggctggat ttgctgagga tgaaggtgga ggaaggtgat gtcattctgg
     3421 tgaagaagct cgaccgtctt ggccgcgaca ccgccgacat gatccaactg ataaaagagt
     3481 ttgatgctca gggtgtagcg gttcggttta ttgacgacgg gatcagtacc gacggtgata
     3541 tggggcaaat ggtggtcacc atcctgtcgg ctgtggcaca ggctgaacgc cggaggatcc
     3601 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3661 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     3721 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     3781 gcggataaca atttcaagga ggtaacagct atgtatcgcc gtcatctgaa acacagtcgc
     3841 gtcaagaact tatttaagtt cgtgagcgct aaaatgaata ccgtctttac ggtagagtct
     3901 gcgcttgaat ttgatacgtg ttttcatctg gaatactcac catccgtgaa attttacgaa
     3961 gcgcagccgg agggttttta ttacgagttc gctggtcgtc aatgcccata tacccctgac
     4021 tttcgtttgg tggatcagaa cgactcggtt tcgtttcttg aaatcaagcc ctctgacaag
     4081 gtggctgatc cagattttct gcatcgcttt cccctgaagc agcagcgcgc cattgaactt
     4141 agttcaccat taaagcttgt tactgaaaaa caaatccgca ttgatcctat cctggggaac
     4201 ttaaagttac ttcaccgcta ttctggcttt caatccttta ctcctttgca tatgcaatta
     4261 ttgggtctgg tgcaaaaatt gggtcgcgta tcactgctgc gtttaagtga ctccatcgac
     4321 gcaccacctg aggaggtatt agcaagcgcg ttatccttaa tcgctcgtgg aattatgcag
     4381 agtgacttaa cggtacagaa gattggcatc agttcgttcg tgtgggctgg cggtcattca
     4441 ggcattgacc atggataagc ataacggcgg tttatttgaa gacgaattcg taattcccca
     4501 accatccaca tctacctcac ccattgacgc catccaagct gtccttccgg ctactgttga
     4561 tagctttcct tatgttttaa aagttgaggc gctgcatcgt cgcgattaca ttctttgggt
     4621 ggagaagaat cttgccgggg gttggacaga gaagaacctt actcccttgt tggcagatgc
     4681 agcgttggtt ttacctcccc ccacgcctaa ttggcgtact ctggcacgct ggcgtaaaat
     4741 ctatattcaa cacggtcgta aacttgtcag tttaatccca aaacatcagg caaaaggtaa
     4801 cgctcgcagt cgccttccac ccagtgatga gctgtttttc gagcaagcag tacatcgtta
     4861 cttagtagga gagcagcctt caattgcgtc ggcattccag ttgtactccg actcaatccg
     4921 tatcgagaat cttggtgtgg tggagaatcc tattaaaact atcagctaca tggctttcta
     4981 caaccgcatt aagaaattac cggcgtatca agtaatgaag agccgtaagg gctcctatat
     5041 tgccgacgtc gagttcaaag ccattgcctc tcacaagcca ccgagtcgta ttatggagcg
     5101 cgtggagatc gatcacacac cattggattt acttttattg gatgatgact tgcttgtgcc
     5161 acttgggcgt ccttccctta cattattgat tgatgcctat tcgcattgcg tggtcggctt
     5221 taatcttaat ttcaatcagc cttcatacga gtccgttcgt aatgcgctgt tatcgagcat
     5281 ctccaaaaaa gattatgtta aaaacaaata cccatccatc gaacacgaat ggccatgcta
     5341 cggtaagcca gagacccttg tcgtggataa cggggtcgag ttttggtccg cttcccttgc
     5401 acaatcgtgc cttgaactgg gaattaacat tcagtacaat cctgttcgca aaccttggtt
     5461 aaagccgatg atcgagcgta tgttcgggat tattaaccgt aagcttctgg aacccattcc
     5521 gggcaaaacc ttcagcaaca tccaggagaa gggtgattat gatccccaaa aagacgctgt
     5581 tatgcgcttt agcacatttc ttgaaatctt tcatcactgg gtcattgacg tgtatcacta
     5641 cgaacctgac agccgttacc gttacattcc gattatctcc tggcaacacg gaaataaaga
     5701 cgccccacct gcaccaatca ttggtgatga tttgacaaag ctggaagtga ttttaagtct
     5761 ttcgttacat tgtacccatc gtcgtggcgg tatccagcgc taccatttgc gctatgattc
     5821 tgacgagtta gcatcatatc gtatgaacta tccagatcag acgcgtggca aacgtaaagt
     5881 tctggtcaaa cttaatcctc gtgatatctc ttatgtgtac gtcttcttgg aggatttagg
     5941 gtcatacatt cgtgtccctt gtatcgaccc gattggttac accaagggtt tatctctgca
     6001 agaacaccag atcaacgtca agttgcatcg cgattttatt aatgagcaga tggatgtggt
     6061 gagtctgagc aaggcacgta tttacctgaa tgatcgcatt aaaaacgagc ttatcgaagt
     6121 gcgtcgcaac atccgtcagc gcaacgtgaa aggggtaaat aagattgcca agtatcgtaa
     6181 tgttggatct cacgcagaaa catccattgt tcatgagctg aaccatcctg cgacgaatga
     6241 ggtcatctct aagatggaga gtgccagcca gcccgaacac tgtgacgatt gggataattt
     6301 cacttctggc ttggagccct actgatggac ctttcgtgtc acgacgcaga caaattgcgc
     6361 tcgtttattg aatgttatgt agaaaccccg cttcttcgcg ctatccagga agacttcgat
     6421 cgtcttcgtt tcaacaagca atttgcaggt gagccgcagt gtatgttgct tactggtgac
     6481 accggcaccg gcaagagctc ccttatccgt cattacgcag ccaaacatcc tgaacaggta
     6541 cgtcacggtt tcattcacaa gccattactt gtttcacgca ttccgtctcg tcccaccctt
     6601 gaatctacca tggtggagtt gcttaaagat ttaggtcaat tcggcagttc tgatcgtatc
     6661 cataaaagtt ccgccgaaag tttaaccgag gctttaatta aatgtcttaa acgctgtgag
     6721 accgagctga ttatcatcga cgagtttcaa gagcttattg aaaataagac ccgcgagaag
     6781 cgcaaccaaa ttgcgaatcg cttgaagtat atttcggaaa cagctaagat tccgattgtt
     6841 ttagtaggta tgccctgggc gaccaagatt gcagaagagc cacaatggag ctctcgcttg
     6901 ttgattcgtc gttcaatccc ctattttaag ttatccgacg atcgtgaaaa ttttatccgc
     6961 cttattatgg gattggcgaa tcgcatgccc tttgagaccc aagcacgcct tgagaccaaa
     7021 cacactatct acgctttgtt tgccgcgtgc tacgggagcc ttcgtgcact gaaacaactg
     7081 ctggacgaat cagttaagca agcgttggcc gcccatgccg agacgctgaa gcatgagcac
     7141 attgccgtgg cttatgcatt attttacccg gatcaggtca atccgttttt acagccgatt
     7201 gacgagatca aggcttgtga ggttaagcaa tatagtcgtt acgaaatcga tgccgccggg
     7261 aaagaggaag tcctgaaccc attacaattt actgataaaa tcccgatttc tcagctgtta
     7321 aaaaaacgat aatgactcga ggc
//
