LOCUS       sequence-322778-        9316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the
            Tn6900 element with an arabinose promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Petassi MT, Hsieh SC, Peters JE
  TITLE     Guide RNA Categorization Enables Target Site Choice in 
            Tn7-CRISPR-Cas Transposons.
  JOURNAL   Cell. 2020 Nov 30. pii: S0092-8674(20)31464-1. doi: 
            10.1016/j.cell.2020.11.005.
  PUBMED    33271061
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Nov 30. pii: S0092-8674(20)31464-1. doi: 
            10.1016/j.cell.2020.11.005."
COMMENT     Sequence Label: pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))
FEATURES             Location/Qualifiers
     source          1..9316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(541..546,547..563,564..569))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      654..1242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1143..1162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1396..1413
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1428..1568
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1494..1513)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1654..1676
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1670..1861)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2350..3228)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3255..3539
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3462..3481
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(8616..8633)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      8786..8872
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8964..8991
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(9135..9316,1..352))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact
       61 tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg
      121 cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg
      181 agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt
      241 tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact
      301 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg
      361 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
      421 atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
      481 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
      541 agtttacgaa ccgaacaggc ttatgtcaat tcgagctcca tgaccaaaat cccttaacgt
      601 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      661 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      721 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      781 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
      841 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      901 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      961 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1021 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1081 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1141 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1201 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1261 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1321 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     1381 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     1441 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     1501 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     1561 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     1621 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     1681 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     1741 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     1801 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     1861 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     1921 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     1981 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     2041 gccagctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa
     2101 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc
     2161 gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt
     2221 taactgtgat aaactaccgc attaaagcta gcttatcgat gcataatgtg cctgtcaaat
     2281 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt
     2341 cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
     2401 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
     2461 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
     2521 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
     2581 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
     2641 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
     2701 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
     2761 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
     2821 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
     2881 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
     2941 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
     3001 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
     3061 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
     3121 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
     3181 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
     3241 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
     3301 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta
     3361 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
     3421 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
     3481 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
     3541 cccgtttttt tgggctagca ggaggaattc accatgcacc ttcttgtacg cccggaaccg
     3601 tttgcggacg aggctttgga atcatacttc ttacgtttgt cacaggagaa tggatttgag
     3661 cgctaccgca ttttctccgg ctccgtacaa gattggcttc atacaacaga tcacgctgcc
     3721 gctggcgcct tccctcttga gctgagtcgt ttaaatatct ttcacgcctc tcgcagttct
     3781 ggtttgcgcg tccgtgctct tcaattggtt gaccgtctta ccgatggcgc cccctttcgc
     3841 ctgcttcagc tggcattatg tcattcggca attagcttcg ggaatcatta caaggctgtt
     3901 catcgcagtg gcgtggatat cccattatct ttcattcgtg tccaccaaat tccttgctgc
     3961 cccgattgcc ttcgcgagag cgcttatgta cgccaatgtt ggcattttaa accgtacgtg
     4021 ggatgccatc gccacggggg ccgcttaatt tattcatgtc cagcatgtgg ggagtctttg
     4081 aactatttag cctcagaatc gattaatcat tgccagtgcg ggttcgactt acgtacggcc
     4141 tcaactgttc ccgcacagcc cgatgaaatt cagttgtcag ccttggccta cggatgctcg
     4201 ttcgagtctt ccaatccgct tcttgcgatc ggctctttat cggcccgttt cggagcttta
     4261 tactggtatc agcagcgtta cctgtcggat cacgaggctg tgcgtgacga tcgtgctttg
     4321 accaaggcga ttggtcactt taccgcttgg cccgatgctt tctggcgtga attgcaacag
     4381 atggtagacg acgccttagt acgccaaact aagccgttga accatactga ctttgtagac
     4441 gtctttggct cggttgttgc tgattgtcgt cagattccca tgcgcaacac gggccaaaat
     4501 ttcattttaa aaaacttgat cggatttctt acagatttgg tggcacgcca ccctcagtgt
     4561 cgcgtagcaa atgtcggcga tcttctgctg tcggcagttg acgcggccac acttctttct
     4621 acttcggtcg agcaggtacg tcgtctgcat cacgaggggt tcttaccctt gtctatccgc
     4681 cctgctagtc gcaataccgt ctctccgcat cgcgcagtct tccatttacg ccacgtcgtc
     4741 gaattgcgtc aagcacgcat gcaatcacat catgatcaca gttcaaccta tctacctgca
     4801 tggtgaccat catgcacatc gaagagttgt tggacatcga agaccatggg gagcgtgatc
     4861 gccaattgcg ccgttacctg gcgccatact ctgcagagat cggggtcgat ggggccgaaa
     4921 agatggcttt agtggtcctt ttgaatctta ctctgaagcg cgaccgtgtt gagagcttat
     4981 gtgacgaggg tctggctcgt cagttactgt ctgatgaagg gcatatcaca aactgcctgc
     5041 acacggttcg ttggttgcac acacacaact taaagtaccc ggatgctcgt gtctcaggcg
     5101 agcgtttaat tattaacgct ccgccattga tcccgggtgt aatctcatcg gcaggcttac
     5161 cgatgcgcat ggggtgggcc catgactcaa gcgatatcaa cttagccaag ctgttcggaa
     5221 caagttttcg ctatcgcgat gactcaacaa atttagcact tcagcttgtg gcgcgtagca
     5281 aaacttggga acaagcctta attggactgg gactgactca gcagcaattg gatatctggt
     5341 gtcaactttt ggcatctaat cttgaaaata acacatttcc cactgttgtg tccccctttt
     5401 ccaaacaggt gcgcttcttg tatcagggta actattgcgt ggttacgccc gtcgtctctc
     5461 acgcccttct tgcgcaattg caaaatgtgg ttcacgaaaa gaagttacag tgtacgtaca
     5521 ttcaccatga ccaccccgcc tcagttggca gtttagtggg ggccttgggt ggtaaagtcg
     5581 ctgtattaga ttacccgccc ccagtttccc cggataaagc tcgtagcttt agccaggccc
     5641 gcaaacatcg cttggcgaat ggtcagagcc tgtttgatcg ttccgtgttt aacgaccatg
     5701 tattcatcga cgcgttaaaa catgtgatta gtcgccccgg attaactcgc aaacaacaac
     5761 gtcaattacg cctttccgca ttgcgttacc ttcgtcgcca gttggccatt tggttaggcc
     5821 ccattattga gtggcgtgat gagattgttt ctagcggtcg tggagaacca ggaaatctgc
     5881 catcaggagg attagagctt gagctgatta cccaaccaaa gaagatgtta cctgagctga
     5941 tgcttcaggt tgcagggcgt tttcatttag agcttcagaa ccacagtgca ggacgccgct
     6001 tcgccttcca tccggcgtta atggctccta tcaaatctca aattctttgg ctgcttcgcc
     6061 aattggccga cgatgaagaa aaggatgaac ctcacccccc caccagttgc tattatttac
     6121 acctttcagg gttgactgtg tatgacgctt cagcattggc gaacccgtac ctgtgtggaa
     6181 tcccatcttt gagcgctctg gctggtttct gtcatgacta tgagcgtcgc ttacagtcct
     6241 taatcgggca gagcgtatat ttccgtgggt tggcgtggta cttggggcgc tactcccttg
     6301 ttaccggcaa gcatttaccc gaaccgtcga aatcagctga ccctaagtct gtttcagcga
     6361 ttcgccgccc aggcttgttg gatgggcgtt actgcgacct gggaatggac cttattatcg
     6421 aagttcacat tcccaccggc ggatcgttgc cgttcacgac atgtctggac ttattgcgtg
     6481 tagccctgcc tgcacgtttc gctggcggct gccttcatcc cccgtccttg tacgaggaat
     6541 ataattggtg cactgtctac caagacaaga gtacgctgtt tacagtcctg tcacgtcttc
     6601 cgcgctacgg ctgttggatt taccccagtg atgcagactt gcgcagcttt gaagagctgt
     6661 cagaagcgtt agccttggac cgtcgcttac gtccggttgc tacaggcttt gtgtttttag
     6721 aagaacccgt ggaacgtgca ggatcgatcg aaggccaaca cgtttatgcc gaaagtgcga
     6781 tcggaactgc cttgtgcatt aatccagtgg agatgcgtct ggctggtaaa aagcgcttct
     6841 tcggcgcagg tttttggcag ttgaacgacg ctaaaggtgc catcctgatg aatggttcag
     6901 caaacacggg gtaaatgcat ggaattatgt actcacttgt catattcccg cagcctgtca
     6961 cccggaaagg ccgtcttctt ctacaaaacg gccgagtcag acttcgtccc cttacgtatc
     7021 gaggttgcta agatctccgg acaaaaatgt ggctatacag aaggttttga tgcaaatttg
     7081 aagccaaaaa acattgagcg ctacgagttg gcgtacagta acccccaaac tatcgaggct
     7141 tgttacgtcc cgccgaacgt agatgaactg tattgccgtt tctcgcttcg cgtcgaagct
     7201 aattccatgc gtccctatgt ttgtagtaat ccagatgtac ttcgtgtgat gattggcctg
     7261 gcgcaggcct accagcgtct gggaggctac aacgaattag ctcgccgtta tagcgcgaat
     7321 gtattacgtg gaatttggtt atggcgcaac caatatactc aggggaccaa aattgaaatc
     7381 aaaacatcat tgggttctac ctatcacatc ccagacgctc gccgcctttc ttggtcgggc
     7441 gactggcctg aactggaaca aaagcaactt gagcagttga cctcagagat ggcgaaagct
     7501 ctttcgcagc ctgacatctt ttggtttgcg gacgttaccg cgtcactgaa gacgggtttt
     7561 tgccaggaaa tctttccttc acaaaaattt acagagcgtc ctgatgatca ctccgttgcc
     7621 tcccgccagc ttgccaccgt ggaatgttca gatggacaac tggcagcctg catcaacccg
     7681 cagaagatcg gcgcggcttt gcaaaagatc gatgactggt gggcgaatga cgcggatctg
     7741 cctctgcgtg tccacgaata cggggctaat catgaagcat taacagcgtt acgccaccct
     7801 gcaacgggtc aagatttcta ccaccttctt actaaggccg aacaatttgt gacggtgctt
     7861 gaatcctctg aaggaggggg cgttgagctg ccgggagagg tacactattt gatggcagtg
     7921 ttggtgaagg gtggattgtt tcagaaaggg aaggggagat gaccgaaaat cgctactttt
     7981 tcgccatccg ttatttgagt gacgatgtgg actgtggtct tttagctgga cgttgcattt
     8041 cgattcttca tgggtttcgc caagcgcacc ctggcattca aatcggtgtc gctttccccg
     8101 aatggagtga tcgcgacctg ggtcgtagca tcgcttttgt ttcgacgaac aagagtctgt
     8161 tagaacgttt ccgtgagcgt tcctactttc aggtcatgca ggcggacaat ttcttcgcgc
     8221 tgtctttggt actggaagtc cccgatactt gtcagaacgt tcgctttatc cgtaatcaaa
     8281 acttagccaa gttgttcgtc ggcgaacgcc gtcgtcgttt ggcgcgtgcc aagcgccgcg
     8341 ctaaggcccg cggtgaagcc ttccaacccc atatgcctga tgagacaaaa gtagttggtg
     8401 tcttccactc cgtattcatg caatcagcaa gctctggtca gtcttacatc ctgcatattc
     8461 agaaacatcg ttatgagcgt tcggaggatt cgggttactc gagttatggc ttagcaagta
     8521 acgacttata tacaggttat gtcccagatc ttggtgcaat cttctctacg ctgttttaag
     8581 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
     8641 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
     8701 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
     8761 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     8821 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     8881 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     8941 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
     9001 acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc atggagctcg
     9061 aattggccgc ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg
     9121 gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc
     9181 cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg
     9241 tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg
     9301 gcaatgccct gcctcc
//
