LOCUS       sequence-322783-       10996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q 
            mutant under EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Liao YC, Fernandopulle MS, Wang G, Choi H, Hao L, Drerup CM, Patel 
            R, Qamar S, Nixon-Abell J, Shen Y, Meadows W, Vendruscolo M, Knowles
            TPJ, Nelson M, Czekalska MA, Musteikyte G, Gachechiladze MA, 
            Stephens CA, Pasolli HA, Forrest LR, St George-Hyslop P, 
            Lippincott-Schwartz J, Ward ME
  TITLE     RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, 
            Using Annexin A11 as a Molecular Tether.
  JOURNAL   Cell. 2019 Sep 19;179(1):147-164.e20. doi: 
            10.1016/j.cell.2019.08.050.
  PUBMED    31539493
REFERENCE   2  (bases 1 to 10996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08"; journalName: "Cell"; date: "2019-09-19- 
            19"; volume: "179"; issue: "1"; pages: "147-164.e20"
COMMENT     Sequence Label: pLEX-EF1a ANXA11-mEmerald R235Q
FEATURES             Location/Qualifiers
     source          1..10996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4958..4960,4961..4963,4964..5677)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5003..5024)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5264..5283)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5611..5632
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5790..6339
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5934..5951)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6161..6180
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6342..6941
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6342..6361)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6838..6858
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6965..7553
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7018..7038)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7436..7447)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7760..8393
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8521..8537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8521..8537)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8534..8556)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8545..8561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8569..8575,8576..8593,8594..8599))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8614..8635
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8752..8769)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8923..9511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9003..9022)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9682..10473,10474..10542))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10305..10324
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10543..10647)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10695..10829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10732..10751)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10786..10805
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatcgccac catgagctac cctggctatc ccccgccccc
     3421 aggtggctac ccaccagctg caccaggtgg tggtccctgg ggaggtgctg cctaccctcc
     3481 tccgcccagc atgcccccca tcgggctgga taacgtggcc acctatgcgg ggcagttcaa
     3541 ccaggactat ctctcgggaa tggcggccaa catgtctggg acatttggag gagccaacat
     3601 gcccaacctg taccctgggg cccctggggc tggctaccca ccagtgcccc ctggcggctt
     3661 tgggcagccc ccctctgccc agcagcctgt tcctccctat gggatgtatc cacccccagg
     3721 aggaaaccca ccctccagga tgccctcata tccgccatac ccaggggccc ctgtgccggg
     3781 ccagcccatg ccaccccccg gacagcagcc cccaggggcc taccctgggc agccaccagt
     3841 gacctaccct ggtcagcctc cagtgccact ccctgggcag cagcagccag tgccgagcta
     3901 cccaggatac ccggggtctg ggactgtcac ccccgctgtg cccccaaccc agtttggaag
     3961 ccgaggcacc atcactgatg ctcccggctt tgaccccctg cgagatgccg aggtcctgcg
     4021 gaaggccatg aaaggcttcg ggacggatga gcaggccatc attgactgcc tggggagtcg
     4081 ctccaacaag cagcagcagc agatcctact ttccttcaag acggcttacg gcaaggattt
     4141 gatcaaagat ctgaaatctg aactgtcagg aaactttgag aagacaatct tggctctgat
     4201 gaagacccca gtcctctttg acatttatga gataaaggaa gccatcaagg gggttggcac
     4261 tgatgaagcc tgcctgattg agatcctcgc ttcccgcagc aatgagcaca tccgagaatt
     4321 aaacagagcc tacaaagcag aattcaaaaa gaccctggaa gaggccattc gaagcgacac
     4381 atcagggcac ttccagcggc tcctcatctc tctctctcag ggaaaccgtg atgaaagcac
     4441 aaacgtggac atgtcactcg cccagagaga tgcccaggag ctgtatgcgg ccggggagaa
     4501 ccgcctggga acagacgagt ccaagttcaa tgcggttctg tgctcccgga gccgggccca
     4561 cctggtagca gttttcaatg agtaccagag aatgacaggc cgggacattg agaagagcat
     4621 ctgccgggag atgtccgggg acctggagga gggcatgctg gccgtggtga aatgtctcaa
     4681 gaatacccca gccttctttg cggagaggct caacaaggcc atgagggggg caggaacaaa
     4741 ggaccggacc ctgattcgca tcatggtgtc tcgcagcgag accgacctcc tggacatcag
     4801 atcagagtat aagcggatgt acggcaagtc gctgtaccac gacatctcgg gagatacttc
     4861 aggggattac cggaagattc tgctgaagat ctgtggtggc aatgacaccg gtggtggagg
     4921 aggttctgga ggcggtggaa gtggtggcgg aggtagcatg gtgagcaagg gcgaggagct
     4981 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     5041 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     5101 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg
     5161 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5221 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     5281 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     5341 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     5401 ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac
     5461 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     5521 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     5581 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     5641 cgggatcact ctcggcatgg acgagctgta caagtaaaat tcctcgaggg cggccgctct
     5701 agagtcgacg ggccgcggta acaattgtta actaacttaa gctagcaacg gtttccctct
     5761 agcgggatca attccgcccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     5821 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     5881 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     5941 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     6001 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     6061 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     6121 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     6181 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     6241 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     6301 ccacggggac gtggttttcc tttgaaaaac acgataatac catgaccgag tacaagccca
     6361 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     6421 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     6481 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6541 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6601 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6661 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6721 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6781 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6841 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6901 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acccgggcgg ggcgcgtctg
     6961 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7021 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7081 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7141 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7201 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7261 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7321 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     7381 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7441 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7501 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     7561 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     7621 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7681 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7741 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7801 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     7861 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     7921 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     7981 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     8041 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     8101 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     8161 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     8221 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8281 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8341 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8401 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8461 gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg
     8521 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8581 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8641 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8701 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8761 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8821 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8881 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8941 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9001 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9061 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9121 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9181 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9241 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9301 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9361 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9421 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9481 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9541 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9601 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9661 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9721 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9781 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9841 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9901 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9961 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10021 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10081 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10141 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10201 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10261 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10321 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10381 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10441 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10501 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10561 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10621 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    10681 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10741 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10801 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    10861 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    10921 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    10981 ctacaaactt agtagt
//
