LOCUS       sequence-322804-        7397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector for Gal-inducible yeast expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Boos F, Kramer L, Groh C, Jung F, Haberkant P, Stein F, Wollweber F,
            Gackstatter A, Zoller E, van der Laan M, Savitski MM, Benes V, 
            Herrmann JM
  TITLE     Mitochondrial protein-induced stress triggers a global adaptive 
            transcriptional programme.
  JOURNAL   Nat Cell Biol. 2019 Apr;21(4):442-451. doi: 
            10.1038/s41556-019-0294-5. Epub 2019 Mar 18.
  PUBMED    30886345
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-019-0294-5"; journalName: "Nat Cell Biol"; date: 
            "2019-04"; volume: "21"; issue: "4"; pages: "442-451"
COMMENT     Sequence Label: pYX233
FEATURES             Location/Qualifiers
     source          1..7397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    301..418
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        complement(819..837)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(858..874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(858..874)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(871..893)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    882..898
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(906..912,913..930,931..936))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1089..1106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1260..1848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1340..1359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2019..2810,2811..2879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFVAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2642..2661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2880..2984)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3261..4603
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    3593..3640
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(5163..5179)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5163..5179)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     5241..5259
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5297..5319)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5419..5438
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5459..5740
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5741..6415
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(6518..6973)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6655..6676)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6867..6886
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7099..7121
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7113..7130
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7114..7130
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7137..7156
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7137..7155
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(7284..7310)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        join(7367..7397,1..634)
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     primer_bind     complement(join(7380..7397,1..6))
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 tatattaaca attttttgtt gatactttta ttacatttga ataagaagta atacaaaccg
       61 aaaatgttga aagtattagt taaagtggtt atgcagtttt tgcatttata tatctgttaa
      121 tagatcaaaa atcatcgctt cgctgattaa ttaccccaga aataaggcta aaaaactaat
      181 cgcattatca tcctatggtt gttaatttga ttcgttcatt tgaaggtttg tggggccagg
      241 ttactgccaa tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt
      301 cggagcagtg cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga
      361 cgcacggagg agagtcttcc ttcggagggc tgtcacccgc tcggcggctt ctaatccgta
      421 cttcaatata gcaatgagca gttaagcgta ttactgaaag ttccaaagag aaggtttttt
      481 taggctaaga taatggggct ctttacattt ccacaacata taagtaagat tagatatgga
      541 tatgtatatg gatatgtata tggtggtaat gccatgtaat atgattatta aacttctttg
      601 cgtccatcca aaaaaaaagt aagaattttt gaaaattcaa tataaatgac agctcagtta
      661 caaagtgaaa gtacttctaa aattgttttg gttacaggtg gtgctggata cattggttca
      721 cacactgtgg tagagctaat tgagaatgga tatgactgtg ttgttgctga taacctgtcg
      781 aattggtccg acggccggcc cggtaccagc ttttgttccc tttagtgagg gttaatttcg
      841 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      901 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      961 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1021 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1081 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1141 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1201 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1261 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1321 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1381 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1441 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1501 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1561 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1621 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1681 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1741 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1801 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1861 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1921 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1981 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2041 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2101 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2161 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2221 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2281 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2341 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2401 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2461 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2521 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2581 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2641 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2701 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2761 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2821 ggaaggcaaa atgccacaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2881 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2941 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3001 gtgccacctg acgtgcggcc gcagctttga agaaaaatgc gccttattca atctttgcta
     3061 taaaaaatgg cccaaaatct cacattggaa gacatttgat gacctcattt ctttcaatga
     3121 agggcctaac ggagttgact aatgttgtgg gaaattggag cgataagcgt gcttctgccg
     3181 tggccaggac aacgtatact catcagataa cagcaatacc tgatcactac ttcgcactag
     3241 tttctcggta ctatgcatat gatccaatat caaaggaaat gatagcattg aaggatgaga
     3301 ctaatccaat tgaggagtgg cagcatatag aacagctaaa gggtagtgct gaaggaagca
     3361 tacgataccc cgcatggaat gggataatat cacaggaggt actagactac ctttcatcct
     3421 acataaatag acgcatataa gtacgcattt aagcataaac acgcactatg ccgttcttct
     3481 catgtatata tatatacagg caacacgcag atataggtgc gacgtgaaca gtgagctgta
     3541 tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc
     3601 tattccgaag ttcctattct ctagaaagta taggaacttc agagcgcttt tgaaaaccaa
     3661 aagcgctctg aagacgcact ttcaaaaaac caaaaacgca ccggactgta acgagctact
     3721 aaaatattgc gaataccgct tccacaaaca ttgctcaaaa gtatctcttt gctatatatc
     3781 tctgtgctat atccctatat aacctaccca tccacctttc gctccttgaa cttgcatcta
     3841 aactcgacct ctacattttt tatgtttatc tctagtatta ctctttagac aaaaaaattg
     3901 tagtaagaac tattcataga gtgaatcgaa aacaatacga aaatgtaaac atttcctata
     3961 cgtagtatat agagacaaaa tagaagaaac cgttcataat tttctgacca atgaagaatc
     4021 atcaacgcta tcactttctg ttcacaaagt atgcgcaatc cacatcggta tagaatataa
     4081 tcggggatgc ctttatcttg aaaaaatgca cccgcagctt cgctagtaat cagtaaacgc
     4141 gggaagtgga gtcaggcttt ttttatggaa gagaaaatag acaccaaagt agccttcttc
     4201 taaccttaac ggacctacag tgcaaaaagt tatcaagaga ctgcattata gagcgcacaa
     4261 aggagaaaaa aagtaatcta agatgctttg ttagaaaaat agcgctctcg ggatgcattt
     4321 ttgtagaaca aaaaagaagt atagattctt tgttggtaaa atagcgctct cgcgttgcat
     4381 ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg
     4441 catttttgtt ttacaaaaat gaagcacaga ttcttcgttg gtaaaatagc gctttcgcgt
     4501 tgcatttctg ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg
     4561 cgttgcattt ttgttctaca aaatgaagca cagatgcttc gttaacaaag atatgctatt
     4621 gaagtgcaag atggaaacgc agaaaatgaa ccggggatgc gacgtgcaag attacctatg
     4681 caatagatgc aatagtttct ccaggaaccg aaatacatac attgtcttcc gtaaagcgct
     4741 agactatata ttattataca ggttcaaata tactatctgt ttcagggaaa actcccaggt
     4801 tcggatgttc aaaattcaat gatgggtaac aagtacgatc gtaaatctgt aaaacagttt
     4861 gtcggatatt aggctgtatc tcctcaaagc gtattcgaat atcattgaga agctgcagcg
     4921 tcacatcgga taataatgat ggcagccatt gtagaagtgc cttttgcatt tctagtctct
     4981 ttctcggtct agctagtttt actacatcgc gaagatagaa tcttagatca cactgccttt
     5041 gctgagctgg atcaatagag taacaaaaga gtggtaaggc ctcgttaaag gacaaggacc
     5101 tgagcggaag tgtatcgtac agtagacgga gtatactagt atagtctata gtccgtggaa
     5161 ttgatccact agttctagag cggccgcact ctcactagta cgtctaagaa accattatta
     5221 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     5281 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     5341 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5401 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catagatcaa
     5461 cgacattact atatatataa tataggaagc atttaataga acagcatcgt aatatatgtg
     5521 tactttgcag ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc
     5581 ttgtcacctt acgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattaa
     5641 ttcggtcgaa aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac
     5701 gtgagtatac gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac
     5761 aggtagttct ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg
     5821 tgctctagat tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac
     5881 aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc
     5941 aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc
     6001 tctggtcaat gattacggca ttgatatagt ccaactgcat ggagatgagt cgtggcaaga
     6061 ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa
     6121 catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga
     6181 agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca
     6241 agagagcccc gaaagtttac attttatgtt agctggtgga ctgacgccag aaaatgttgg
     6301 tgatgcgctt agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa
     6361 tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat
     6421 tactgagtag tatttattta agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat
     6481 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg
     6541 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     6601 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     6661 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     6721 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     6781 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     6841 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     6901 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     6961 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     7021 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     7081 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     7141 acgactcact atagggcgaa ttggagctag acaaagacaa aaaaaaaaag ggatgtatcg
     7201 gtcagtcatt aatactagga acaagagaaa ggaaaaacgt tcattgttcc ttattcagtt
     7261 agctagctga gctcgagata tcatgcgtag tcaggcacat catacggata cccgggtcga
     7321 cgcgtaagct tgtgggccct aggatccatg gtgaattcga tccggggttt tttctccttg
     7381 acgttaaagt atagagg
//
