LOCUS       sequence-322823-        7883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses in Candida albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Van Genechten W, Demuyser L, Dedecker P, Van Dijck P
  TITLE     Presenting a codon-optimized palette of fluorescent proteins for use
            in Candida albicans.
  JOURNAL   Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.1038/s41598-020-63308-w.
  PUBMED    32273559
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-04-9"; volume: "10(1):6158. doi"; pages: " 
            10.1038/s41598-020-63308-w"
COMMENT     Sequence Label: CIp10 - mTurquoise2 (gamma-mGFP based)
FEATURES             Location/Qualifiers
     source          1..7883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..726
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(743..759)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(744..760)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1169..1187)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1208..1224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1208..1224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1221..1243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1232..1248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1256..1262,1263..1280,1281..1286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1301..1322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1439..1456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1610..2198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1690..1709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2369..3160,3161..3229))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2992..3011
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3230..3334)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3361..3816
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3448..3467)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3658..3679
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3942..3964
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3956..3973
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3957..3973
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3980..3999
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3980..3998
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgctgcaga tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa
       61 ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct
      121 acttacggta aattgacctt aaaatttatt tgtactactg gtaaattgcc agttccatgg
      181 ccaaccttag tcactacttt atcatggggt gttcaatgtt ttgctagata cccagatcat
      241 atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact
      301 atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat
      361 accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta
      421 ggtcacaaat tggaatacaa ctatttttct gataatgttt acatcactgc tgacaaacaa
      481 aagaatggta tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg tggtgttcaa
      541 ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac
      601 aaccattact tatccactca atctaaatta tccaaagatc caaacgaaaa gagagaccac
      661 atggtcttgt tagaatttgt tactgctgct ggtattacct taggtatgga tgaattgtac
      721 aaataactag tagcgacgtc atcgataccg tcgacctcga ggagtgaaat tctggaaatc
      781 tggaaatctg gttttgtatt cttgttattc ttctttttgt tattacatat ataacttgtt
      841 acttttttaa aaaaatcttt gtttatttta taaatatata aaactaaatt taagaaaaag
      901 agaaaaatgt tttatttgag agattgaaat tttacttgaa tttagcttag cttttataaa
      961 gtattattat gtaaaaaaac aaaacaaata tacattaaaa agttaagact ataaaatagc
     1021 cacccaaggc atttctatat cttgttgttg ttgttttcat cttctgtatc agaggaactt
     1081 attttattat tttcgtcacg ggtattttct cttgtttgat gattcatccc attcattcca
     1141 tcaaaaatgt cggtaccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt
     1201 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1261 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1321 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     1381 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2041 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2281 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2341 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2401 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2461 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2521 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2581 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2641 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2701 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2761 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2821 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2881 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2941 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3001 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3061 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3121 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3181 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3241 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3301 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3361 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3421 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3481 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3541 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3601 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3661 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     3721 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3781 acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa
     3841 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     3901 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     3961 aacgacggcc agtgaattgt aatacgactc actatagggc gaattggagc tccaccgcgg
     4021 tggcggccgc gtttataata aacttagtct aatatttctt ttggaccaaa atagaaactg
     4081 ttctcaggtg gcaaaaaaca gcttaaagtc tgtgttaaaa aaagatctcg gctatgccaa
     4141 tcaaaaagga gtgacattat tatgtaaaag aagaaatgtt gtaattccgc gaaacaacaa
     4201 ttttaatgct tactgtgccg ccaccaatat cctatggcca aggggccttt aaacatgcca
     4261 cggaaatata cagtcaaagt aaacctacta tgtctttaga gccttcagga tcaattgttt
     4321 ttaaatgaat gactgcaaaa ctagaaaagt agcctcttac ataagcaagt catttgtaca
     4381 ccgagagttt agtaaccagt gaagcaagta tatctggcaa acctgaaact tatctgtgaa
     4441 ttataaagct cataagtaaa aagtgtagaa aagaagataa ggcagaccaa atacaaattt
     4501 gcaacagtac aacgaatatt tcagcgtcaa aactagagaa taataaagaa aacgatcttt
     4561 tcaaaaagaa aaaacctttt agttttcctt tgttgttgtt gtgggtgtgt gctatttata
     4621 ttatatagtt tactcataat accataaaat attcggtttg attaggttat tttaataagc
     4681 taatttgttt ctaatcgtgt aatttatgct gtgtatatta agtagtgtgt gcactgccca
     4741 aaaatgtttg ttgtttatag tcggttaaag agaaaaaaga aaaaaagatc catacacaca
     4801 cgttaattag ttgttcaacg taatacactc atattttgtt cttatttgct ttcggtcgct
     4861 gttctcacca agatttattg ccaacgaaac aatttttttt tatatatttt cagatttttc
     4921 tttttttcct ttcctttcct tttctaattt tcactcctgg ttttctttct ttcttagaaa
     4981 cattatctcg atattaatat taaaaaaata taatcattca aactccggaa tgtctactac
     5041 tttggatgat actgcttata gatacagaac ttctgttcca ggtgatgctg aagctattga
     5101 agctttggat ggttctttca ctaccgatac tgtttttaga gttactgcta ctggtgatgg
     5161 tttcactttg agagaagttc cagttgatcc accattgact aaggttttcc cagatgatga
     5221 atccgatgat gaatccgatg ctggtgaaga tggtgatcca gattctagaa ctttcgttgc
     5281 ttatggtgat gatggtgatt tggctggttt cgttgttgtt tcttattctg gttggaacag
     5341 aagattgact gttgaagata ttgaagttgc tccagaacat agaggtcatg gtgttggtag
     5401 agctttgatg ggtttggcta ctgaattcgc cagagaaaga ggtgctggtc atttgtggtt
     5461 ggaagttacc aatgttaatg ctccagctat tcatgcttat agaagaatgg gtttcacttt
     5521 gtgtggtttg gatactgctt tatacgatgg tactgcttcc gatggtgaac aagctttgta
     5581 tatgtctatg ccatgtccat aataactcgg ggggatccga gtgaaattct ggaaatctgg
     5641 aaatctggtt ttgtattctt gttattcttc tttttgttat tacatatata acttgttact
     5701 tttttaaaaa aatctttgtt tattttataa atatataaaa ctaaatttaa gaaaaagaga
     5761 aaaatgtttt atttgagaga ttgaaatttt acttgaattt agcttagctt ttataaagta
     5821 ttattatgta aaaaaacaaa acaaatatac attaaaaagt taagactata aaatagccac
     5881 ccaaggcatt tctatatctt gttgttgttg ttttcatctt ctgtatcaga ggaacttatt
     5941 ttattatttt cgtcacgggt attttctctt gtttgatgat tcatcccatt cttccatcat
     6001 aaaatactag tggatccccc agatccttat ccttaaacag attctaaaac aacatctttg
     6061 aaaccagaag aaactttctt acctttttct tcggttgaac cttcaccgtg caaagccaat
     6121 aatgaaccca agtcgaattt tggttgtttc aacaatttga cttttctgat gtggacattt
     6181 tgtaatggga aaatggtttg ggtggatttt tcaatttcac ggccaatgac ttctggaatc
     6241 aatttggaag ttaattgagc taaagtacag ttggaaactt ctctttgcat aatttcaatc
     6301 atcttctttc tgacttctct caatttagag gattgagcgt aagtagtttt cttgatttgg
     6361 tttggttgtc ttttggtgaa agcaatggca aaaactctca aaacgtaatc gtcggaagtt
     6421 ttaacagtga cattagcttc aactaatgat tgccattttc tgaccaatga tcttaatttg
     6481 tcagaagtga aatccaaacc atgaggcctc catgaggcct ccatgaggcc tccatgaggc
     6541 ctccatggag gcctcatgga agttggtcaa caagttttta ccttggactt catcaactct
     6601 caatttgatt tttctgtaag agtggtcttc ggaaccttgt aagtcggcca aacaaacttc
     6661 gaaaactcta cctttcaagc catcagcggc attctttaaa ccggtagatc tgttgatcaa
     6721 agttttacca acatttctgt tttcaaaagt ggttggagct ttgatgtcaa accaatcttt
     6781 tctggtgaat gggtcaacga ccttcttttt taatcctttc tttcctttgg acaatctctt
     6841 gtttttaccg acagccatgt tgtacttgag ttggatctac gcgtcagcct cgtttataat
     6901 aaacttagtc taatatttct tttggaccaa aatagaaact gttctcaggt ggcaaaaaac
     6961 agcttaaagt ctgtgttaaa aaaagatctc ggctatgcca atcaaaaagg agtgacatta
     7021 ttatgtaaaa gaagaaatgt tgtaattccg cgaaacaaca attttaatgc ttactgtgcc
     7081 gccaccaata tcctatggcc aaggggcctt taaacatgcc acggaaatat acagtcaaag
     7141 taaacctact atgtctttag agccttcagg atcaattgtt tttaaatgaa tgactgcaaa
     7201 actagaaaag tagcctctta cataagcaag tcatttgtac accgagagtt tagtaaccag
     7261 tgaagcaagt atatctggca aacctgaaac ttatctgtga attataaagc tcataagtaa
     7321 aaagtgtaga aaagaagata aggcagacca aatacaaatt tgcaacagta caacgaatat
     7381 ttcagcgtca aaactagaga ataataaaga aaacgatctt ttcaaaaaga aaaaaccttt
     7441 tagttttcct ttgttgttgt tgtgggtgtg tgctatttat attatatagt ttactcataa
     7501 taccataaaa tattcggttt gattaggtta ttttaataag ctaatttgtt tctaatcgtg
     7561 taatttatgc tgtgtatatt aagtagtgtg tgcactgccc aaaaatgttt gttgtttata
     7621 gtcggttaaa gagaaaaaag aaaaaaagat ccatacacac acgttaatta gttgttcaac
     7681 gtaatacact catattttgt tcttatttgc tttcggtcgc tgttctcacc aagatttatt
     7741 gccaacgaaa caattttttt ttatatattt tcagattttt ctttttttcc tttcctttcc
     7801 ttttctaatt ttcactcctg gttttctttc tttcttagaa acattatctc gatattaata
     7861 ttaaaaaaat ataatcattc aaa
//
