LOCUS       sequence-322826-       11544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Liao YC, Fernandopulle MS, Wang G, Choi H, Hao L, Drerup CM, Patel 
            R, Qamar S, Nixon-Abell J, Shen Y, Meadows W, Vendruscolo M, Knowles
            TPJ, Nelson M, Czekalska MA, Musteikyte G, Gachechiladze MA, 
            Stephens CA, Pasolli HA, Forrest LR, St George-Hyslop P, 
            Lippincott-Schwartz J, Ward ME
  TITLE     RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, 
            Using Annexin A11 as a Molecular Tether.
  JOURNAL   Cell. 2019 Sep 19;179(1):147-164.e20. doi: 
            10.1016/j.cell.2019.08.050.
  PUBMED    31539493
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08"; journalName: "Cell"; date: "2019-09-19- 
            19"; volume: "179"; issue: "1"; pages: "147-164.e20"
COMMENT     Sequence Label: PLEX-PGK-ANXA11-mCerulean
FEATURES             Location/Qualifiers
     source          1..11544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        2717..3227
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3146..3164
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(4791..4793,4794..4796,4797..5510)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4836..4857)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5097..5116)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5444..5465
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5519..6107
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5572..5592)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5990..6001)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6314..6547
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6648..6665)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6654..6878
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6924..7352
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7011..7030)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7221..7242
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7361..7381)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7366..7695
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7546..7681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7608..7627
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7744..8769
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7744..8769
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8899..9020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9069..9085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9069..9085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9082..9104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9093..9109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9117..9123,9124..9141,9142..9147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9162..9183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9300..9317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9471..10059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9551..9570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10230..11021,11022..11090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10853..10872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11091..11195)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11243..11377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gaccacgggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     2761 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     2821 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     2881 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     2941 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     3001 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     3061 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     3121 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     3181 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg atccgcggcc
     3241 gcgccaccat gagctaccct ggctatcccc cgcccccagg tggctaccca ccagctgcac
     3301 caggtggtgg tccctgggga ggtgctgcct accctcctcc gcccagcatg ccccccatcg
     3361 ggctggataa cgtggccacc tatgcggggc agttcaacca ggactatctc tcgggaatgg
     3421 cggccaacat gtctgggaca tttggaggag ccaacatgcc caacctgtac cctggggccc
     3481 ctggggctgg ctacccacca gtgccccctg gcggctttgg gcagcccccc tctgcccagc
     3541 agcctgttcc tccctatggg atgtatccac ccccaggagg aaacccaccc tccaggatgc
     3601 cctcatatcc gccataccca ggggcccctg tgccgggcca gcccatgcca ccccccggac
     3661 agcagccccc aggggcctac cctgggcagc caccagtgac ctaccctggt cagcctccag
     3721 tgccactccc tgggcagcag cagccagtgc cgagctaccc aggatacccg gggtctggga
     3781 ctgtcacccc cgctgtgccc ccaacccagt ttggaagccg aggcaccatc actgatgctc
     3841 ccggctttga ccccctgcga gatgccgagg tcctgcggaa ggccatgaaa ggcttcggga
     3901 cggatgagca ggccatcatt gactgcctgg ggagtcgctc caacaagcag cggcagcaga
     3961 tcctactttc cttcaagacg gcttacggca aggatttgat caaagatctg aaatctgaac
     4021 tgtcaggaaa ctttgagaag acaatcttgg ctctgatgaa gaccccagtc ctctttgaca
     4081 tttatgagat aaaggaagcc atcaaggggg ttggcactga tgaagcctgc ctgattgaga
     4141 tcctcgcttc ccgcagcaat gagcacatcc gagaattaaa cagagcctac aaagcagaat
     4201 tcaaaaagac cctggaagag gccattcgaa gcgacacatc agggcacttc cagcggctcc
     4261 tcatctctct ctctcaggga aaccgtgatg aaagcacaaa cgtggacatg tcactcgccc
     4321 agagagatgc ccaggagctg tatgcggccg gggagaaccg cctgggaaca gacgagtcca
     4381 agttcaatgc ggttctgtgc tcccggagcc gggcccacct ggtagcagtt ttcaatgagt
     4441 accagagaat gacaggccgg gacattgaga agagcatctg ccgggagatg tccggggacc
     4501 tggaggaggg catgctggcc gtggtgaaat gtctcaagaa taccccagcc ttctttgcgg
     4561 agaggctcaa caaggccatg aggggggcag gaacaaagga ccggaccctg attcgcatca
     4621 tggtgtctcg cagcgagacc gacctcctgg acatcagatc agagtataag cggatgtacg
     4681 gcaagtcgct gtaccacgac atctcgggag atacttcagg ggattaccgg aagattctgc
     4741 tgaagatctg tggtggcaat gacaccggag gaggaagtgg cggaagtggc atggtgagca
     4801 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4861 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4921 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4981 ccctgagctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     5041 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5101 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5161 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5221 acaacgccat ccacggcaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     5281 ccaacttcgg cctcaactgc aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5341 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5401 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5461 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga cgcgtaacaa
     5521 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5581 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5641 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5701 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5761 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5821 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5881 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     5941 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6001 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6061 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     6121 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     6181 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     6241 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6301 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     6361 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6421 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6481 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6541 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6601 atatcagaga gtgagaggcc ttgacattgt ttaaacccgc tgatcagcct cgactgtgcc
     6661 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     6721 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6781 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6841 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     6901 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6961 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7021 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7081 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7141 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7201 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7261 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7321 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7381 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7441 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7501 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7561 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7621 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7681 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     7741 gtgatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     7801 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     7861 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     7921 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     7981 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     8041 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     8101 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     8161 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     8221 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     8281 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     8341 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     8401 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     8461 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     8521 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     8581 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     8641 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     8701 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     8761 aaggaatagc acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8821 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8881 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8941 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9001 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     9061 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9121 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9181 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9241 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9301 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9361 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9421 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9481 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9541 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9601 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9661 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9721 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9781 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9841 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9901 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9961 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10021 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10081 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10141 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10201 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10261 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10321 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10381 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10441 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10501 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10561 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10621 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10681 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10741 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10801 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10861 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10921 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10981 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11041 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11101 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11161 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11221 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
    11281 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11341 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    11401 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    11461 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    11521 aatatgtact acaaacttag tagt
//
