LOCUS       sequence-322833-       11470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Liao YC, Fernandopulle MS, Wang G, Choi H, Hao L, Drerup CM, Patel 
            R, Qamar S, Nixon-Abell J, Shen Y, Meadows W, Vendruscolo M, Knowles
            TPJ, Nelson M, Czekalska MA, Musteikyte G, Gachechiladze MA, 
            Stephens CA, Pasolli HA, Forrest LR, St George-Hyslop P, 
            Lippincott-Schwartz J, Ward ME
  TITLE     RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, 
            Using Annexin A11 as a Molecular Tether.
  JOURNAL   Cell. 2019 Sep 19;179(1):147-164.e20. doi: 
            10.1016/j.cell.2019.08.050.
  PUBMED    31539493
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08"; journalName: "Cell"; date: "2019-09-19- 
            19"; volume: "179"; issue: "1"; pages: "147-164.e20"
COMMENT     Sequence Label: PLEX-PGK-LAMP1-HaloTag
FEATURES             Location/Qualifiers
     source          1..11470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        2717..3227
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3146..3164
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3241..3250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3245..3254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4543..5433
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     misc_feature    5445..6033
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5498..5518)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5916..5927)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6240..6473
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6574..6591)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6580..6804
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6850..7278
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6937..6956)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7147..7168
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7287..7307)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7292..7621
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7472..7607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7534..7553
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7670..8695
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7670..8695
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8825..8946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8995..9011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8995..9011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9008..9030)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9019..9035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9043..9049,9050..9067,9068..9073))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9088..9109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9226..9243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9397..9985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9477..9496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10156..10947,10948..11016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10779..10798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11017..11121)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11169..11303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gaccacgggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     2761 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     2821 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     2881 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     2941 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     3001 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     3061 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     3121 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     3181 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg atccgcggcc
     3241 gccaccatgg cggcccccgg cagcgcccgg cggccgctgc tcctgttgct gctggcaggc
     3301 cttgcacaca gcgccccagc actgttcgag gtgaaagaca acaacggcac agcgtgtata
     3361 atggccagct tctctgcctc ctttctgacc acctatgagg ctggacatgt ttctaaggtc
     3421 tcgaatatga ccctgccagc ctctgcagaa gtcctgaaga atagcagctc ttgtggtgaa
     3481 aagaatgctt ctgagcccac cctcgcaatc acctttggag aaggatattt actgaaactc
     3541 accttcacaa aaaacacaac acgttacagt gtccagcaca tgtatttcac atataacctg
     3601 tcagacacac aattctttcc caatgccagc tccaaagggc ccgacactgt ggattccaca
     3661 actgacatca aggcagacat caacaaaaca taccgatgtg tcagcgacat cagggtctac
     3721 atgaagaatg tgaccattgt gctctgggac gctactatcc aggcctacct gccgagtagc
     3781 aacttcagca aggaagagac acgctgccca caggatcaac cttccccaac tactgggcca
     3841 cccagcccct caccaccact tgtgcccaca aaccccagtg tgtccaagta caatgtgact
     3901 ggtgacaatg gaacctgcct gctggcctct atggcactgc aactcaacat cacctacatg
     3961 aagaaggaca acacgactgt gaccagagca ttcaacatca acccaagtga caaatatagt
     4021 gggacttgcg gtgcccagtt ggtgaccctg aaggtgggga acaagagcag agtcctggag
     4081 ctgcagtttg ggatgaatgc cacttctagc ctgtttttcc tgcaaggagt tcagttgaac
     4141 atgactcttc ctgatgccat agagcccacg ttcagcacct ccaactattc cctgaaagct
     4201 cttcaggcca gtgtcggcaa ctcatacaag tgcaacagtg aggagcacat ctttgtcagc
     4261 aaggcgctcg ccctcaatgt cttcagcgtg caagtccagg ctttcagggt agaaagtgac
     4321 aggtttgggt ctgtggaaga gtgtgtacag gacggtaaca acatgctgat ccccattgct
     4381 gtgggcgggg ccctggcagg gctggtcctc atcgtcctca tcgcctacct catcggcagg
     4441 aagaggagtc acgcgggcta tcagaccatc tcggaattcg gctccaccgg ctccacaggc
     4501 tccaccggcg cggtcgacgg tggaggaagt ggcggaagtg gcatggcaga aatcggtact
     4561 ggctttccat tcgaccccca ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat
     4621 gttggtccgc gcgatggcac ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac
     4681 gtgtggcgca acatcatccc gcatgttgca ccgacccatc gctgcattgc tccagacctg
     4741 atcggtatgg gcaaatccga caaaccagac ctgggttatt tcttcgacga ccacgtccgc
     4801 ttcatggatg ccttcatcga agccctgggt ctggaagagg tcgtcctggt cattcacgac
     4861 tggggctccg ctctgggttt ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt
     4921 gcatttatgg agttcatccg ccctatcccg acctgggacg aatggccaga atttgcccgc
     4981 gagaccttcc aggccttccg caccaccgac gtcggccgca agctgatcat cgatcagaac
     5041 gtttttatcg agggtacgct gccgatgggt gtcgtccgcc cgctgactga agtcgagatg
     5101 gaccattacc gcgagccgtt cctgaatcct gttgaccgcg agccactgtg gcgcttccca
     5161 aacgagctgc caatcgccgg tgagccagcg aacatcgtcg cgctggtcga agaatacatg
     5221 gactggctgc accagtcccc tgtcccgaag ctgctgttct ggggcacccc aggcgttctg
     5281 atcccaccgg ccgaagccgc tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac
     5341 atcggcccgg gtctgaatct gctgcaagaa gacaacccgg acctgatcgg cagcgagatc
     5401 gcgcgctggc tgtcgacgct cgagatttcc ggctagcgcg taacaatcaa cctctggatt
     5461 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5521 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5581 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5641 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5701 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5761 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5821 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     5881 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5941 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6001 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
     6061 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     6121 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     6181 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact
     6241 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     6301 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6361 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6421 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     6481 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6541 gaggccttga cattgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     6601 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6661 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6721 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6781 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     6841 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6901 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6961 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7021 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7081 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7141 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7201 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7261 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7321 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7441 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7501 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7561 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7621 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtga tgaaaaagcc
     7681 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     7741 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     7801 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     7861 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     7921 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     7981 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     8041 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     8101 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     8161 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     8221 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     8281 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     8341 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     8401 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     8461 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     8521 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     8581 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     8641 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt
     8701 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8761 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8821 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8881 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8941 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9001 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9061 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9121 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9181 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10261 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10741 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10861 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10921 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10981 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11041 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11101 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    11161 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    11221 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    11281 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    11341 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    11401 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    11461 acttagtagt
//
