LOCUS       sequence-322842-        1153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses reversibly switchable A-kinase activity reporter with 
            EV-linker (rsAKARev) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Roebroek T, Vandenberg W, Sipieter F, Hugelier S, Stove C, Zhang J, 
            Dedecker P
  TITLE     Simultaneous readout of multiple FRET pairs using photochromism.
  JOURNAL   Nat Commun. 2021 Mar 31;12(1):2005. doi: 10.1038/s41467-021-22043-0.
  PUBMED    33790271
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-31"; volume: "12(1):2005. doi"; pages: " 
            10.1038/s41467-021-22043-0"
COMMENT     Sequence Label: pcDNA3.1-rsAKARev
FEATURES             Location/Qualifiers
     source          1..1153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..812
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTNAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTLKWEPSTEILYVRDGVLV
                     GDIKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARYSTGGMDELYK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tcccatggtg agcaagggcg
      121 aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca
      181 acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca
      241 ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc
      301 tgaccaacgc cttcgcttac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca
      361 actacttcaa gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg
      421 acaagggcat cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg
      481 agatacacct caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagaccc
      541 tgaagtggga gccctccacc gagatcctgt acgtgcgcga cggcgtgctg gtgggcgaca
      601 tcaagcacaa gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct
      661 acagggccaa gaaggcggtg aagctgcccg actaccactt tgtggaccac cgcatcgaga
      721 tcctgaacca cgacaaggac tacaacaagg tgaccgttta cgagagcgcc gtggcccgct
      781 actccaccgg cggcatggac gagctgtaca agagatctat gcataagttt tctcaagaac
      841 agatcggcga aaacattgtg tgcagggtca tttgtaccac gggtcaaatt cccatccgag
      901 atttgtcagc tgatatttca caagtgctta aggaaaaacg atccataaag aaagtttgga
      961 catttggtag aaacccagcc tgtgactatc atttaggaaa catttcaaga ctgtcaaata
     1021 agcatttcca aatactacta ggagaagacg gtaacctttt attgaatgac atttccacta
     1081 atgggacctg gttaaatggg caaaaagtcg agaagaacag caatcagtta ctgtcccaag
     1141 gtgatgaaat aac
//
