LOCUS       sequence-322843-        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses reversibly switchable A-kinase activity reporter with 
            EV-linker (rsAKARev) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Roebroek T, Vandenberg W, Sipieter F, Hugelier S, Stove C, Zhang J, 
            Dedecker P
  TITLE     Simultaneous readout of multiple FRET pairs using photochromism.
  JOURNAL   Nat Commun. 2021 Mar 31;12(1):2005. doi: 10.1038/s41467-021-22043-0.
  PUBMED    33790271
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-31"; volume: "12(1):2005. doi"; pages: " 
            10.1038/s41467-021-22043-0"
COMMENT     Sequence Label: pcDNA3.1-rsAKARev
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(109..130)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(310..657)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
ORIGIN
        1 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatag atccaccggt
       61 acctcccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      121 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact ggtagctcag
      181 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      241 gtcggcgagc tgcacgccgc ccatgagctc gctgccgccg tcaaccagcg tcgcgcgcct
      301 caatccggaa ccaccagcac taccaccagc actaccacca gcactaccac cagcactacc
      361 accagcactg gtactaccac cagcactacc accagcactg ccggaaccac cagcactacc
      421 accagcacta ccaccagcac taccaccagc actaccacca gcactggtac taccaccagc
      481 actaccacca gcactgccgg aaccaccagc actaccacca gcactaccac cagcactacc
      541 accagcacta ccaccagcac tggtactacc accagcacta ccaccagcac tgccggaacc
      601 accagcacta ccaccagcac taccaccagc actaccacca gcactaccac cagcactggt
      661 accgcgatca actttgttct gctccaggca ctgcttaaat ttgtcgttta tgaaaatgac
      721 cagagataaa atatctgatt ccacgcctac accaacggtt atttcatcac cttgggacag
      781 taactgattg ctgttcttct cgactttttg cccatttaac caggtcccat tagtggaaat
      841 gtcattcaat aaaaggttac cgtcttctcc tagtagtatt tggaaatgct tatttgacag
      901 tcttgaaatg tttcctaaat gatagtcaca ggctgggttt ctaccaaatg tccaaacttt
      961 ctttatggat cgtttttcct taagcacttg tgaaatatca gctgacaaat ctcggatggg
     1021 aatttgaccc gtggtacaaa tgaccctgca cacaatgttt tcgccgatct gttcttgaga
     1081 aaacttatgc atagatctct tgtacagctc gtccatgccg ccggtggagt agcgggcc
//
