LOCUS       sequence-322844-        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses reversibly switchable A-kinase activity reporter with 
            EV-linker (rsAKARev) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Roebroek T, Vandenberg W, Sipieter F, Hugelier S, Stove C, Zhang J, 
            Dedecker P
  TITLE     Simultaneous readout of multiple FRET pairs using photochromism.
  JOURNAL   Nat Commun. 2021 Mar 31;12(1):2005. doi: 10.1038/s41467-021-22043-0.
  PUBMED    33790271
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-31"; volume: "12(1):2005. doi"; pages: " 
            10.1038/s41467-021-22043-0"
COMMENT     Sequence Label: pcDNA3.1-rsAKARev
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(61..795)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     254..273
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     513..534
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(640..661)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc agaattctta
       61 ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt
      121 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      181 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      241 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      301 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      361 ggcgaagcac tgaaggccgt agcccagggt ggtcacgagg gtgggccagg gcacgggcag
      421 cttgccggtg gtgcagatca gcttcagggt cagcttgccg taggtggcat cgccctcgcc
      481 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      541 caccaccccg gtgaacagct cctcgccctt gctcaccata gatccaccgg tacctccctt
      601 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
      661 gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tggtagctca ggtagtggtt
      721 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
      781 ctgcacgccg cccatgagct cgctgccgcc gtcaaccagc gtcgcgcgcc tcaatccgga
      841 accaccagca ctaccaccag cactaccacc agcactacca ccagcactac caccagcact
      901 ggtactacca ccagcactac caccagcact gccggaacca ccagcactac caccagcact
      961 accaccagca ctaccaccag cactaccacc agcactggta ctaccaccag cactaccacc
     1021 agcactgccg gaaccaccag cactaccacc agcactacca ccagcactac caccagcact
     1081 accaccagca ctggtactac caccagcac
//
