LOCUS       sequence-322846-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses reversibly switchable A-kinase activity reporter with 
            EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Roebroek T, Vandenberg W, Sipieter F, Hugelier S, Stove C, Zhang J, 
            Dedecker P
  TITLE     Simultaneous readout of multiple FRET pairs using photochromism.
  JOURNAL   Nat Commun. 2021 Mar 31;12(1):2005. doi: 10.1038/s41467-021-22043-0.
  PUBMED    33790271
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-31"; volume: "12(1):2005. doi"; pages: " 
            10.1038/s41467-021-22043-0"
COMMENT     Sequence Label: pcDNA3.1-rsAKARev(T/A)
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             99..806
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTNAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTLKWEPSTEILYVRDGVLV
                     GDIKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARYSTGGMDELYK"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagcttg gtaccgagct cggatcccat ggtgagcaag ggcgaggaga
      121 ccacaatggg cgtaatcaag cccgacatga agatcaagct gaagatggag ggcaacgtga
      181 atggccacgc cttcgtgatc gagggcgagg gcgagggcaa gccctacgac ggcaccaaca
      241 ccatcaacct ggaggtgaag gagggagccc ccctgccctt ctcctacgac attctgacca
      301 acgccttcgc ttacggcaac agggccttca ccaagtaccc cgacgacatc cccaactact
      361 tcaagcagtc cttccccgag ggctactctt gggagcgcac catgaccttc gaggacaagg
      421 gcatcgtgaa ggtgaagtcc gacatctcca tggaggagga ctccttcatc tacgagatac
      481 acctcaaggg cgagaacttc ccccccaacg gccccgtgat gcagaagaag accctgaagt
      541 gggagccctc caccgagatc ctgtacgtgc gcgacggcgt gctggtgggc gacatcaagc
      601 acaagctgct gctggagggc ggcggccacc accgcgttga cttcaagacc atctacaggg
      661 ccaagaaggc ggtgaagctg cccgactacc actttgtgga ccaccgcatc gagatcctga
      721 accacgacaa ggactacaac aaggtgaccg tttacgagag cgccgtggcc cgctactcca
      781 ccggcggcat ggacgagctg tacaagagat ctatgcataa gttttctcaa gaacagatcg
      841 gcgaaaacat tgtgtgcagg gtcatttgta ccacgggtca aattcccatc cgagatttgt
      901 cagctgatat ttcacaagtg cttaaggaaa aacgatccat aaagaaagtt tggacatttg
      961 gtagaaaccc agcctgtgac tatcatttag gaaacatttc aagactgtca aataagcatt
     1021 tccaaatact actaggagaa gacggtaacc ttttattgaa tgacatttcc actaatggga
     1081 cctggttaaa tgggcaaaaa gtcgagaaga acagcaatca gttactgtcc caaggtgatg
     1141 aaataaccgt tggt
//
