LOCUS       sequence-32286-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLKA-J72026
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             56..64
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             73..114
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(141..158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(220..244)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        291..395
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(614..633)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctacttccta ggactgaagt ggaattcatg agatctgaag caatatcttc acgtataagg
       61 aggtatttca tgggtaagcc tatccctaac cctctgctgg gtctcgattc tacgggatcc
      121 taactcgctc ctcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      181 cgagcggtat cagctcactc aaaggcggta atcaattcga cccagctttc ttgtacaaag
      241 tggttgatcg gatctcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      301 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      361 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      421 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      481 tgaaagtaaa agatgcagag gatcagttgg gtgcacgagt gggttacatc gaactggatc
      541 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      601 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      661 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      721 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      781 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
      841 ttt
//
