LOCUS       sequence-32288-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72028
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(75..92)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(154..178)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(328..347)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(412..431)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ggatctgaag tggaattcat gagatctcac catcatcacc atcactaagg atcctaactc
       61 gctcctcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      121 gtatcagctc actcaaaggc ggtaatcaat tcgacccagc tttcttgtac aaagtggttg
      181 atcggatctg ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg
      241 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
      301 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
      361 cgtcttgctc caggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
      421 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg
      481 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
      541 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
      601 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc
      661 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
      721 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
      781 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
      841 acga
//
