LOCUS       sequence-32289-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72029
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..76
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(106..123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(185..209)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(359..378)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(443..462)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gctaggatct gaagtggaat tcatgagatc tggcctgaac gatatttttg aagcgcagaa
       61 aattgaatgg catgaataag gatcctaact cgctcctcag gcttcctcgc tcactgactc
      121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatcaa
      181 ttcgacccag ctttcttgta caaagtggtt gatcggatct gctgatcctt caactcagca
      241 aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca
      301 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
      361 aggggtgtta tgagccatat tcaacgggaa acgtcttgct ccaggccgcg attaaattcc
      421 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
      481 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
      541 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
      601 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
      661 accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt
      721 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
      781 aattgtcc
//
