LOCUS       sequence-32290-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72030
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(97..114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(176..200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(350..369)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(434..453)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggatctgaa gtggaattca tgagatctgg tgctccggtt ccatatccag atccactgga
       61 accacgttaa ggatcctaac tcgctcctca ggcttcctcg ctcactgact cgctgcgctc
      121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatca attcgaccca
      181 gctttcttgt acaaagtggt tgatcggatc tgctgatcct tcaactcagc aaaagttcga
      241 tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa
      301 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
      361 atgagccata ttcaacggga aacgtcttgc tccaggccgc gattaaattc caacatggat
      421 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      481 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      541 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      601 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      661 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      721 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      781 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      841 gttgatgcga gtgattttga tgacgagcgt aa
//
