LOCUS       sequence-32291-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-K112603
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(82..99)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(161..185)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(335..354)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(419..438)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggatgattt ctggaattca tgagatctga ctacaaggat gacgacgaca agtaaggatc
       61 ctaactcgac gtgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      121 gcgagcggta tcagctcact caaaggcggt aatcaattcg acccagcttt cttgtacaaa
      181 gtggttgatc ggatctgctg atccttcaac tcagcaaaag ttcgatttat tcaacaaagc
      241 cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
      301 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
      361 cgggaaacgt cttgctccag gccgcgatta aattccaaca tggatgctga tttatatggg
      421 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
      481 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
      541 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
      601 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca
      661 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
      721 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
      781 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
      841 tt
//
