LOCUS       sequence-322910-        3849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of TVA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Faulkner RL, Wall NR, Callaway EM, Cline HT
  TITLE     Application of Recombinant Rabies Virus to Xenopus Tadpole Brain.
  JOURNAL   eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2021. doi: 
            10.1523/ENEURO.0477-20.2021.
  PUBMED    34099488
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/ENEURO.0477-20"; journalName: "eNeuro"; date: "2021-06-7- 
            7"; volume: "8"; issue: "4"; pages: "ENEURO.0477-20.2021"
COMMENT     Sequence Label: UAS::TVA
FEATURES             Location/Qualifiers
     source          1..3849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     131..153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     145..162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     146..162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1213..1232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1214..1232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1246..1262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1246..1262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1259..1281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1270..1286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1294..1300,1301..1318,1319..1324))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1339..1360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1477..1494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1648..2236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1728..1747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2407..3198,3199..3267))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3030..3049
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3268..3372)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3399..3827
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3486..3505)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3696..3717
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
       61 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      121 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct aattaccctg
      181 ttatccctag gcggaccgaa gcttgcatgc ctgcaggtcg actctagagg atcccacgtg
      241 cccgggcgtt taaaccaatt gttcgaaagc ttaggcctcc aaggcggagt actgtcctcc
      301 gggctggcgg agtactgtcc tccggcaagg tcggagtact gtcctccgac actagaggtc
      361 ggagtactgt cctccgacgc aaggcggagt actgtcctcc gggctgcgga gtactgtcct
      421 ccggcaaggt cggagtactg tcctccgaca ctagaggtcg gagtactgtc ctccgacgca
      481 aggtcggagt actgtcctcc gacactagag gtcggagtac tgtcctccga cgcaaggtcg
      541 gagtactgtc ctccgacact agaggtcgga gtactgtcct ccgacgcaag gcggagtact
      601 gtcctccggg ctggcggagt actgtcctcc ggcaagggtc gactctagag ggtatataat
      661 ggatcccatc gcgtctcagc ctcactttga gctcctccac acgctcgaga tggcgcggct
      721 gctgcccgcg ctgctgctgc tgctgctgcc cggtaacgtg accggtaacg ggtccggtaa
      781 cggttctttg tcccgttgcc cccccggtca gttccgctgc tcggagccgc ccggtgccca
      841 cggggagtgt tacccgcagg actggctgtg cgacggacac cccgactgcg acgacgggcg
      901 ggacgagtgg ggctgcggga ccagcgcgac ccccgcggtg cccacggaca acggcacaga
      961 ggctcccact gtccctgctc ctggacgtgc tctgccagcc aggaatcacg gccgcatgtg
     1021 gatgctgatc actgcaggga tcttttgctg tgagctggtg agatgggact gaggtaccga
     1081 gctcgaattc gcggccgcgg gcccatatgg cccaccggtg ggccggccag atctgctagc
     1141 actagtggcg ccacgcgtga tatcatgcat gttaacatcg atccatgggc gcgccttaat
     1201 taaatttaaa ttccctatag tgagtcgtat taaattcgta atcatgtcat agctgtttcc
     1261 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1321 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1381 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1441 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1501 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1561 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2221 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2281 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2341 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2401 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2521 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3001 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3241 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3361 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     3421 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3481 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3541 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3601 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3661 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3721 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     3781 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3841 aacgcttac
//
