LOCUS       sequence-322917-       10933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-tagged Mitochondria-associated ER membrane marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-PTDSS1-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..10933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(3536..3538,3539..3541,3542..4252)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3536..3538,3539..3541,3542..4252)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3581..3602)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3842..3861)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4189..4210
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(5683..6257,6258..6260,6261..6269)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5850..5867)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6077..6096
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6289..6888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6289..6308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6785..6805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6902..7490
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6955..6975)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7373..7384)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7697..8330
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8458..8474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8458..8474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8471..8493)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8482..8498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8506..8512,8513..8530,8531..8536))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8551..8572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8689..8706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8860..9448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8940..8959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9619..10410,10411..10479))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10242..10261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10480..10584)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10632..10766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10669..10688)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10723..10742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgatggt
     3541 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3601 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3661 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3721 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3781 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3841 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3901 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3961 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     4021 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     4081 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     4141 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     4201 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agatggcgtc
     4261 ctgcgtgggg agccggaccc taagcaagga tgatgtgaac tacaaaatgc atttccggat
     4321 gatcaacgag cagcaagtgg aggacatcac cattgacttc ttctaccggc cgcataccat
     4381 caccctgctc agcttcacca tcgtcagcct catgtacttc gcctttacca gggatgactc
     4441 tgttccagaa gacaacatct ggagaggcat cctctctgtt attttcttct ttcttatcat
     4501 cagtgtgtta gctttcccca atggtccgtt cactcgacct catccagcct tatggcgaat
     4561 ggtttttgga ctcagtgtgc tctacttcct gttcctggta ttcctactct tcctgaattt
     4621 cgagcaggtt aaatctctaa tgtattggct agatccaaat cttcgatacg ccacaaggga
     4681 agcagatgtc atggagtatg ctgtgaactg ccatgtgatc acctgggaga ggattatcag
     4741 ccactttgat atttttgcat ttggacattt ctggggctgg gccatgaagg ccttgctgat
     4801 ccgtagttac ggtctctgct ggacaatcag tattacctgg gagctgactg agctcttctt
     4861 catgcatctc ctccccaatt ttgccgagtg ctggtgggat caagtcattc tggacatcct
     4921 gttgtgcaat ggcggtggca tttggctggg catggtcgtt tgccggtttt tagagatgag
     4981 gacttaccac tgggcaagct tcaaggacat tcataccacc accgggaaga tcaagagagc
     5041 tgttctgcag ttcactcctg ctagctggac ctatgttcga tggtttgacc ccaaatcttc
     5101 ttttcagaga gtagctggag tgtacctttt catgatcatc tggcagctga ctgagttgaa
     5161 taccttcttc ttgaagcata tctttgtgtt ccaagccagt catccattaa gttggggtag
     5221 aattctcttt attggtggca tcacagctcc cacagtgaga cagtactacg cttacctcac
     5281 cgacacacag tgcaagcgcg taggaacaca atgctgggtg tttggggtca ttggtttcct
     5341 ggaggccatt gtttgcataa aatttggaca agatctcttc tctaagaccc aaatactcta
     5401 tgttgtgctt tggcttcttt gcgtggcttt caccactttc ctctgtctgt acggcatgat
     5461 ttggtatgca gaacactatg gtcaccgaga aaagacctac tcggagtgtg aagatggcac
     5521 ctacagtcca gagatctcct ggcatcacag gaaagggaca aaaggttctg aagacagccc
     5581 acccaagcat gcaggcaaca acgaaagcca ttcttccagg agaaggaatc ggcattccaa
     5641 gtcaaaagtc accaatggcg ttggaaagaa atgaggatcc cgcccctctc cctccccccc
     5701 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     5761 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     5821 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     5881 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     5941 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     6001 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     6061 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     6121 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     6181 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     6241 aaaaacacga tgataagctt gccacaaccc acaaggagac gaccttccat gaccgagtac
     6301 aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc
     6361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag
     6421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6481 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctagac gcgtctggaa
     6901 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6961 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7021 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7081 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7141 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7201 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7261 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7321 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7381 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7441 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     7501 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     7561 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     7621 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7681 aaagaaaaga ggggactgga aggactaatt cactcccaac gaagacaaga tatccttgat
     7741 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     7801 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     7861 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     7921 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     7981 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     8041 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     8101 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     8161 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8221 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8281 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8341 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8401 gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8461 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8521 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8581 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8641 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8761 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8821 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9061 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9181 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9241 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9301 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9361 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9421 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9481 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9541 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9601 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9661 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9721 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9781 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9841 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9901 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9961 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10021 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10081 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10141 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10201 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10261 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10321 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10381 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10441 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10501 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10561 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    10621 atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10681 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10741 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    10801 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    10861 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    10921 caaacttagt agt
//
