LOCUS       sequence-322926-       12724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA,
            donor DNA; non-targeting crRNA has BsaI sites for cloning. 
            Temperature-sensitive pSC101* backbone can be cured by 37 (o)C 
            incubation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg SH
  TITLE     CRISPR RNA-guided integrases for high-efficiency, multiplexed 
            bacterial genome engineering.
  JOURNAL   Nat Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y.
  PUBMED    33230293
REFERENCE   2  (bases 1 to 12724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y."
COMMENT     Sequence Label: pSL1521 (pSPIN, pSC101* backbone)
FEATURES             Location/Qualifiers
     source          1..12724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        241..332
                     /gene="bla"
                     /label=AmpR promoter
     CDS             333..1124
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     1286..1303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1310..1344
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             8326..8337
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9772..9790)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9786..9833
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             9988..10647
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10054..10073)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(10847..10864)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10847..10863)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10856..10878)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11470..11692
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             11740..12690
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 tcaagactaa caaacaaaag tagaacaact gttcaccgtt acatatcaaa gggaaaactg
       61 tccataccca tggattcttc gtctgtttct actggtattg gcacaaacct gatatcgatg
      121 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      181 attcaaatat gtatccgctc atgagacaat aaccctggta gcgaccgagt gagctagcta
      241 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      301 aaatgcttca ataatattga aaaaggaaga gtatgaggga agcggtgatc gccgaagtat
      361 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
      421 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
      481 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
      541 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
      601 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
      661 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
      721 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
      781 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
      841 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
      901 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
      961 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     1021 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     1081 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcct gtcagcagac
     1141 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1201 taggtgaaga tccgcctgca tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1261 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagctt tgacagctag
     1321 ctcagtccta ggtataatac tagcgtcgac gtggagatat accatgggtg aactgccgag
     1381 taggtagctg ataacgagac ctctgtcttg tcagctaggg tggtctcgtg aactgccgag
     1441 taggtagctg ataacggatc cgaattcgag cgaaggagat atacatatgt ttttgcaaag
     1501 acctaaacct tacagcgatg aaagtttaga aagtttcttt atccgagtgg ctaacaaaaa
     1561 tggctacggt gatgtccatc gcttcctaga agccactaaa cgattccttc aagacattga
     1621 ccataatggc tatcaaacct ttccgactga tataactcgg ataaacccat actcagctaa
     1681 aaacagttcc agcgcacgaa ctgcgtcatt cctgaaactt gcacaattga catttaatga
     1741 accgccagag ctacttgggt tggcaattaa cagaacaaac atgaaatact cgccgtcaac
     1801 tagcgcggtt gttagaggtg cagaagtctt tcctcgcagt ttactacgga cgcactccat
     1861 cccctgctgt cctttgtgtc tgcgagaaaa tggctacgcc tcctaccttt ggcactttca
     1921 ggggtacgaa tactgccaca gccataacgt acctttaatt accacttgta gctgtggtaa
     1981 ggagtttgac taccgagtat ctgggttaaa gggcatttgc tgcaaatgca aggagcctat
     2041 caccttaacc agcagggaga acggtcatga ggcagcgtgt actgtttcaa actggcttgc
     2101 tggccatgaa tctaaacctc tgccaaatct tcctaaaagc taccgatggg gtttagttca
     2161 ttggtggatg ggtattaaag atagcgagtt cgatcacttt tcgttcgttc aatttttctc
     2221 aaactggcca aggtcattcc actcgataat cgaagatgaa gtagagttca accttgagca
     2281 tgctgttgtc agcacgtctg aattacgact aaaagatctt cttggtcgat tgtttttcgg
     2341 ttcaattcgg ttacctgagc ggaatcttca acacaatatc atccttggtg agcttctctg
     2401 ctatttagaa aatcgtttat ggcaagacaa gggattaatc gccaacctca aaatgaacgc
     2461 gttagaggcg actgtaatgt taaattgtag cctcgatcag attgcatcaa tggttgaaca
     2521 acgcatcttg aagccaaatc gaaaaagcaa gcccaacagc cctcttgatg ttaccgatta
     2581 tctatttcat ttcggcgata ttttctgtct ttggttagct gagttccaaa gcgatgagtt
     2641 taaccgttcg ttttatgtgt cgaggtggta aatgcaaact ctgaaagaac taatcgcatc
     2701 caatcctgac gacttaacaa ctgaacttaa gagagcattt cgtccactca caccgcatat
     2761 tgcaattgat ggtaatgaac ttgacgcact gacgatatta gtcaatttaa ccgataagac
     2821 tgatgatcag aaagacctgc tcgatcgagc caaatgcaag caaaaacttc gagatgaaaa
     2881 atggtgggct agctgcataa attgcgttaa ttacagacaa agccataacc caaaattccc
     2941 ggatatacgt tctgaaggcg tgattcgaac ccaagccctg ggtgaattac cgagtttcct
     3001 actctcgtcc tcaaaaatcc caccatacca ttggtcatat agccatgatt caaaatacgt
     3061 caacaaaagc gcattcctca ccaatgagtt ttgttgggat ggtgagatct catgtttggg
     3121 tgagcttctt aaagatgcag atcacccact ttggaatact ttgaaaaagt taggttgttc
     3181 tcaaaaaact tgcaaagcaa tggcaaaaca actagctgat attactctca cgactatcaa
     3241 tgtcacactc gcaccaaatt acctgactca aatatctctc cccgatagtg atacatccta
     3301 catttcactc tctcctgttg catcgctatc gatgcaaagc cactttcatc agaggctcca
     3361 agatgaaaat cggcatagcg cgataacgcg gtttagccga actaccaaca tgggggtcac
     3421 tgcgatgaca tgtggcggcg catttagaat gttaaagtct ggcgctaagt tttccagccc
     3481 ccctcatcac cgattaaaca gtaaacgaag ttggttgacg tcagagcatg ttcagtcatt
     3541 aaaacagtac cagcgcctca acaaaagcct catacctgaa aactctcgga ttgcactccg
     3601 tagaaaatac aaaatcgagc ttcaaaatat ggtcagatct tggtttgcaa tgcaagacca
     3661 tacccttgat tcgaacatac ttatccaaca tttgaatcat gacctatctt acttaggagc
     3721 cacaaaacgt tttgcatacg atccagcgat gaccaagctc tttactgagc ttttgaaacg
     3781 agagttatca aattcaatca ataatggtga gcaacacact aatggatcgt ttttagtcct
     3841 accgaatatc agggtttgtg gcgcaacagc tttaagctcc ccggtaacgg tggggattcc
     3901 atcacttaca gctttctttg gcttcgttca cgcatttgaa cggaatataa atcgcaccac
     3961 ctcatcgttt cgtgttgaat cctttgcgat atgcgtccat caactacatg tcgagaagcg
     4021 aggtttgaca gcagagtttg tggaaaaagg cgacgggact atatccgctc ccgcgacccg
     4081 ggatgactgg cagtgtgatg tcgtatttag ccttattttg aacaccaact ttgctcaaca
     4141 tattgaccaa gatacgttag ttacatcact accaaagcga ttggctcggg gttcagcaaa
     4201 aattgcgatt gatgacttta aacatatcaa ctcattctcg acattagaaa cagcgatcga
     4261 atctctgcca atagaagctg gtaggtggtt atcactttac gcacagtcaa acaataatct
     4321 aagtgatcta ttagcagcca tgacagagga ccatcagctc atggcaagct gcgtcggtta
     4381 ccacttgtta gaagagccca aagataaacc aaactccctc agaggttaca aacacgctat
     4441 cgccgagtgc atcattggac tcattaactc aatcaccttt agctcagaga ctgatcccaa
     4501 cacaatcttt tggtcgctaa agaactatca aaactaccta gtggtacagc caaggagtat
     4561 caacgatgaa actaccgaca aatctagcct atgagcgctc tatcgaccca tcagatgtct
     4621 gtttttttgt cgtctggccc gatgatagaa aaacaccttt aacctacaat tctcgtactc
     4681 tgctcgggca aatggaagcg gcatcattag cctatgatgt ctcaggtcaa ccaataaaaa
     4741 gtgccaccgc tgaggcgtta gctcaaggga accctcatca agttgatttc tgccacgttc
     4801 catacggtgc gagtcatatt gaatgcagtt tctccgtctc gttttcttct gaactacgtc
     4861 aaccatataa gtgtaactca agcaaagtta aacaaacgct agtgcaatta gtcgagctct
     4921 acgaaacgaa aatcggctgg actgagctag caacccgata tttgatgaac atttgcaacg
     4981 gtaaatggct gtggaaaaat acccgtaaag cctattgctg gaacattgta cttacacctt
     5041 ggccttggaa cggggaaaag gttggatttg aagatattcg tactaactac acctcacggc
     5101 aagactttaa aaataataaa aattggtctg ctatagttga aatgatcaaa accgcatttt
     5161 ctagtactga tgggctggcg atatttgaag tcagggccac cttgcacttg ccaacgaatg
     5221 ctatggtgcg gccaagccaa gttttcacag aaaaagaaag tggcagtaaa agtaaatcta
     5281 aaactcaaaa cagtcgagtt tttcagagta caactattga tggtgaacga tcgccaatac
     5341 taggggcctt taaaacggga gcagctattg caaccattga cgactggtat cctgaagcca
     5401 ctgagccact aagggtcgga cggtttgggg ttcatcgcga agatgtcact tgctaccgtc
     5461 atccgtctac cggaaaagat tttttctcga tattacaaca agcagagcac tatattgaag
     5521 tgttgagcgc caacaaaact cccgctcaag aaactatcaa cgacatgcac tttttaatgg
     5581 ctaacctgat taagggtggg atgttccagc ataaaggaga ctgactgtga aatggtatta
     5641 taagacaatc acctttctgc cagagttgtg caacaacgag tcactggctg caaagtgtct
     5701 ccgcgttctg catggattta actatcagta tgagacacga aatatcggcg tttcatttcc
     5761 gctttggtgt gatgcaacgg ttggaaaaaa gatttcattt gtcagcaaga acaagataga
     5821 actcgactta ctacttaaac aacactattt cgtccaaatg gaacaacttc aatactttca
     5881 tatatccaac actgttctcg tcccagaaga ttgtacatac gtttccttta gacgctgtca
     5941 atctatagat aagctcacag cagcagggct ggcaaggaaa atcagacgcc tggagaaacg
     6001 tgctctatct agaggcgagc aatttgaccc atcatctttt gctcaaaaag agcatactgc
     6061 aatagcgcac taccactcac ttggggagtc cagcaaacag acgaaccgca actttcgact
     6121 caatatcagg atgctctcgg agcaaccccg tgaggggaac tcgattttta gtagctatgg
     6181 cttatcaaat tcagaaaact cgtttcagcc tgtaccctta atctgacctt taataaggag
     6241 atataccatg gcgacaagtt tacctacgcc ctcagcaatt acgacttcgg cgttagagta
     6301 tgcattccat actcccgctc gcaatctaac gaaatctcgc ggaaaaaaca ttcatcgtta
     6361 tgtcagtgta aagatgagta agaggattac ggtagaatct actctagagt gtgatgcctg
     6421 ctatcacttt gattttgagc caagtattgt tcgcttttgc gctcaaccga ttcgattttt
     6481 atattatctc aatggtcagt ctcactccta tgttcctgac tttctagttc aatttgatac
     6541 caacgagttt gttctatatg aagtaaagtc agcttatgct aagaacaaac ctgattttga
     6601 tgttgaatgg gaggcgaaag taaaagcagc aactgaacta gggctagaat tggagcttgt
     6661 tgaagagagt gatattaggg atacggttgt attaaataat cttaagcgca tgcatcgtta
     6721 tgcttcgaaa gatgagctga ataacgtaca taactctctc ttaaaaataa taaagtacaa
     6781 tggcgcccaa tctgcaagat gcttgggaga acagttgggt ttaaaaggcc gaactgtttt
     6841 accaattttg tgcgatttgc tgtcaaggtg tttactcgat acacgtttgg ataagcctct
     6901 atctcttgaa tctcgatttg agttggccag ttatggctaa gaaagggttc tcaagtttcc
     6961 atagaaaagc agtctcttcc caagatacgc ttgaatcgat agagcttgtc tctagcgcta
     7021 attgtctaga aagtgttacg tatcaagata tatcagcatt tcccgaaaca attgcggtag
     7081 agattaattt ccgattaagc attcttcgct ttttagcgcg aaagtgcgaa accattgtgg
     7141 ccaaatcaat tgaaccacat cgtgtagagc tacagcaaaa ctatagtaga aaaataccca
     7201 gtgcaataac gatatatcga tggtggcttg cttttcgaaa atcagactac aaccccatta
     7261 gcttagcacc taatatcaag gatagaggta atagagagac aaaagtgtca acagttgttg
     7321 attctattat ggaacaggca gttgaaagag ttatatctgg acgaaaagtc aatgttagct
     7381 ctgcatataa acgtgttcga cgaaaagttc gtcaatacaa tctaactcat ggaacgaaat
     7441 acacgtatcc taagtacgaa tctgtaagaa agcgagtaaa aaagaaaacc ccatttgagt
     7501 tattagccgc agggaaaggg gagagagtag ctaagagaga gtttcgccga atgggaaaaa
     7561 agatcctcac gtctagcgtg ctagagaggg ttgaaataga tcacactgtc gttgaccttt
     7621 ttgcagtaca cgaagagtat cgaatcccat tgggccgacc ttggcttact caattggttg
     7681 attgttacag taaagctgta atcggttttt atttaggttt cgagcctcct agctatgtgt
     7741 cggtttccct tgcacttaag aatgcaatac aacgcaaaga tgacttaatc tcctcgtatg
     7801 aatcgatcga gaatgaatgg ctatgttatg gcatcccaga cctactcgta actgataatg
     7861 gtaaagagtt tttgtcgaaa gcatttgatc aagcatgtga atcactattg atcaatgtgc
     7921 atcaaaataa agttgagacg cccgacaaca aacctcatgt tgaacgtaac tacgggacta
     7981 ttaatacttc tctgttagac gatttacctg ggaaatcctt cagccagtac cttcaaagag
     8041 aagggtacga ctctgtggga gaagctaccc ttacactcaa tgagattaga gaaatttact
     8101 taatttggtt ggtggatatt tatcataaaa aacccaatca gagaggcact aattgtccta
     8161 atgttgcgtg gaaaaagggt tgtcaagaat gggaaccaga ggagttctct ggttctaaag
     8221 acgaattaga ctttaaattt gctattgttg attacaaaca acttactaaa gtagggataa
     8281 ctgtctacaa agaactgagt tatagcaatg accgtttagc tgaatataga gggaagaaag
     8341 gaaaccataa agttcagttc aagtataacc ctgagtgtat ggcagttatt tgggtgttgg
     8401 atgaggatat gaatgagtac tttacagtta atgcgattga ctacgaatat gcaagtagag
     8461 tatcactttg gcaacataaa tataacatga aatatcaagc agaactaaat tcagcagaat
     8521 atgatgagga caaggaaatt gatgcagaaa taaaaattga agaaatcgca gatcgttcaa
     8581 ttgttaagac taacaaaatc agagctcgga ggcgtggcgc taggcatcaa gagaatagcg
     8641 caagggctaa gtcaatcagt aatgcgaacc cggcctcgat acaaaaacat gaagatgaaa
     8701 tcgttagtgc agataatgac gattgggata ttgattatgt ctgagaaatc gtcaaatgag
     8761 tgaaacgcgt gaggctcgta tatcaagagc taaaagggca tttgtatcca caccgagcgt
     8821 taggaaaatc ttgagttaca tggatagatg tagagatcta tcagacctag agtctgagcc
     8881 tacatgcatg atggtctatg gtgcttcggg tgtaggtaaa acgaccgtca tcaagaaata
     8941 cttaaatcaa aacagaagag agtccgaagc cgggggcgat ataataccgg ttttgcatat
     9001 tgagttgcca gacaatgcga agccagtaga tgcagcaagg gaattgctgg ttgaaatggg
     9061 tgacccgcta gcactttatg aaactgactt agctagattg acgaaaagac tgactgaatt
     9121 aatccctgcg gtcggcgtga agctgattat tatcgatgag ttccaacatt tggtggaaga
     9181 aaggtcaaat cgggttctta cccaagtagg taattggcta aaaatgatac ttaacaaaac
     9241 gaaatgtcca attgttatat ttggtatgcc atactcaaaa gttgtactgc aagcaaactc
     9301 gcaacttcac gggcgatttt ccattcaggt tgaactgcgc ccctttagct accagggagg
     9361 tagaggtgta tttaaaactt ttttggaata ccttgataaa gccctacctt ttgaaaaaca
     9421 ggctggctta gccaacgaaa gtttgcagaa aaaattgtat gcattctctc agggaaacat
     9481 gcgttcgttg agaaacctta tttatcaagc atctatcgaa gcaattgata atcagcatga
     9541 gacgataacc gaagaagatt tcgtttttgc atcgaagttg acatcgggcg ataaacccaa
     9601 ctcatggaaa aatccttttg aggagggtgt tgaggtaaca gaagatatgt tacgaccgcc
     9661 accaaaagat attggttggg aggactattt gagacattca accccgagag tgagtaaacc
     9721 aggtagaaat aaaaactttt tcgaataacc taggctgctg cgctgctgcc accgctgagc
     9781 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaac
     9841 ctcaggcatt tgttgttgat acaaccataa aatgataatt acacccataa attgataatt
     9901 atcacaccca taaattgata ttgcctcttc atggtctaaa cttcagtaag tttacgacat
     9961 tttcctcgag gtcatttccg gggatccatg gagaaaaaaa tcactggata taccaccgtt
    10021 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
    10081 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
    10141 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
    10201 gagttccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
    10261 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
    10321 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
    10381 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
    10441 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
    10501 actatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
    10561 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
    10621 tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttctaga
    10681 gtccttactg cagtagtttt gctgaaatac tcgattcaca aaaatatcaa cttatggttg
    10741 ttttgtgaga tatcaatata tggttgtttt gtggttaagt tgctgattat aaataattat
    10801 taaatatcac tttatggttg catcaacagg taccgagctc gaattcactg gccgtcgttt
    10861 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gccagttcac
    10921 cgacaaacga aagctgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10981 tgagcgtcag accccgttga tgataccgct gccttactgg gtgcattagc cagtctgaat
    11041 gacctgtcac gggataatcc gaagtggtca gactggaaaa tcagagggca ggaactgctg
    11101 aacagcaaaa agtcagatag caccacatag cagacccgcc ataaaacgcc ctgagaagcc
    11161 cgtgacgggc ttttcttgta ttatgggtag tttccttgca tgaatccata aaaggcgcct
    11221 gtagtgccat ttacccccat tcactgccag agccgtgagc gcagcgaact gaatgtcacg
    11281 aaaaagacag cgactcaggt gcctgatggt cggagacaaa aggaatattc agcgatttgc
    11341 ccgattgcgg ccgcaaccga gcttgcgagg gtgctactta agcctttagg gttttaaggt
    11401 ctgttttgta gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac
    11461 taaagtagtg agttatacac agggctggga tctattcttt ttatcttttt ttattctttc
    11521 tttattctat aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg
    11581 gaatttacag agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta
    11641 cagataccca caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat
    11701 tggtatagtg attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc
    11761 aaagcaaatg aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt
    11821 ttatgctgtg tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg
    11881 gtatcgttca cttataacca atacgttcag atgatgaaca tcagtaggga aaatgcttat
    11941 ggtgtattag ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct
    12001 ttggttaaag gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa
    12061 ttagaattag tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata
    12121 aaatataatc tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag
    12181 tggttattaa aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt
    12241 gatgaattta agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac
    12301 caatgggttt tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg
    12361 gttgataagc gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa
    12421 atggatctcg taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca
    12481 acaaccatta catcagattc ctacctacat aacggactaa gaaaaacact acacgatgct
    12541 ttaactgcaa aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt
    12601 aagtatgatc tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag
    12661 aacatactgg ctaaatacgg aaggatctga ggttcttatg gctcttgtat ctatcagtga
    12721 agca
//
