LOCUS       sequence-322929-        4829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes HIV-1 Tat accessory protein for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
COMMENT     Sequence Label: pHDM-Tat1b
FEATURES             Location/Qualifiers
     source          1..4829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(523..539)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(524..540)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    633..1027
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(1361..1379)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1447..1551
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1552..1620,1621..2412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1770..1789)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2583..3171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3072..3091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3835..4214
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4215..4418
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4369..4389
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4415..4439
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          join(4532..4829,1..178)
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 tataaatatt tctgcatata aattgtaact gatgtaagag gtttcatatt gctaatagca
       61 gctacaatcc agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct
      121 gagtccaagc taggcccttt tgctaatcat gttcatacct cttatcttcc tcccacagct
      181 cctgggcaac gtgctggtct gtgtgctggc ccatcacttt ggcaaagaat tccgcgggcg
      241 gccgcgaaat ggagccagta gatcctagac tagagccctg gaagcatcca ggaagtcagc
      301 ctaaaactgc ttgtaccact tgctattgta aaaagtgttg ctttcattgc caagtttgtt
      361 tcacaacaaa agccttaggc atctcctatg gcaggaagaa gcggagacag cgacgaagac
      421 ctcctcaagg cagtcagact catcaagttt ctctatcaaa gcaacccacc tcccaacccc
      481 gaggggaccc gacaggcccg aaggaatagg atccaagctt atcgataccg tcgacctcga
      541 gggcccagat ctaattcacc ccaccagtgc aggctgccta tcagaaagtg gtggctggtg
      601 tggctaatgc cctggcccac aagtatcact aagctcgctt tcttgctgtc caatttctat
      661 taaaggttcc tttgttccct aagtccaact actaaactgg gggatattat gaagggcctt
      721 gagcatctgg attctgccta ataaaaaaca tttattttca ttgcaatgat gtatttaaat
      781 tatttctgaa tattttacta aaaagggaat gtgggaggtc agtgcattta aaacataaag
      841 aaatgaagag ctagttcaaa ccttgggaaa atacactata tcttaaactc catgaaagaa
      901 ggtgaggctg caaacagcta atgcacattg gcaacagccc ctgatgccta tgccttattc
      961 atccctcaga aaaggattca agtagaggct tgatttggag gttaaagttt tgctatgctg
     1021 tattttacat tacttattgt tttagctgtc ctcatgaatg tcttttcact acccatttgc
     1081 ttatcctgca tctctcagcc ttgactccac tcagttctct tgcttagaga taccaccttt
     1141 cccctgaagt gttccttcca tgttttacgg cgagatggtt tctcctcgcc tggccactca
     1201 gccttagttg tctctgttgt cttatagagg tctacttgaa gaaggaaaaa cagggggcat
     1261 ggtttgactg tcctgtgagc ccttcttccc tgcctccccc actcacagtg acccggaatc
     1321 cctcgacatg gcagtctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
     1381 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     1441 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1501 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1561 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1621 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1681 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1741 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1801 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1861 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1921 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1981 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2041 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2101 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2161 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2221 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2281 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2341 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2401 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2461 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2521 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2581 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2641 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2701 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2761 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2821 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2881 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2941 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3001 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3061 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3121 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3181 aacggagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca
     3241 agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt
     3301 ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat
     3361 gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc
     3421 aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg
     3481 atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca
     3541 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
     3601 agaatcataa tggggaaggc catccagcct cgcgtcgggg agctttttgc aaaagcctag
     3661 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     3721 ctctgcataa ataaaaaaaa ttagtcagcc atgagcttgg cccattgcat acgttgtatc
     3781 catatcataa tatgtacatt tatattggct catgtccaac attaccgcca tgttgacatt
     3841 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     3901 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     3961 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     4021 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     4081 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     4141 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     4201 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     4261 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     4321 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     4381 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     4441 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
     4501 tcccctcgaa gctgatcctg agaacttcag ggtgagtcta tgggaccctt gatgttttct
     4561 ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta acagggtaca
     4621 catattgacc aaatcagggt aattttgcat ttgtaatttt aaaaaatgct ttcttctttt
     4681 aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc tttcagggca
     4741 ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag tgataatttc
     4801 tgggttaagg caatagcaat atttctgca
//
