LOCUS       sequence-32293-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72031
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..61
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     primer_bind     complement(94..111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(173..197)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(347..366)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(431..450)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggatctgaa gtggaattca tgagatctca gccggagctc gcaccagaag acccggaaga
       61 ctgctaagga tcctaactcg ctcctcaggc ttcctcgctc actgactcgc tgcgctcggt
      121 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatcaatt cgacccagct
      181 ttcttgtaca aagtggttga tcggatctgc tgatccttca actcagcaaa agttcgattt
      241 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
      301 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
      361 agccatattc aacgggaaac gtcttgctcc aggccgcgat taaattccaa catggatgct
      421 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
      481 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
      541 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
      601 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
      661 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
      721 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
      781 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaa
//
