LOCUS       sequence-322930-        8389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 Spike Protein for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
COMMENT     Sequence Label: pHDM-SARS-CoV-2
FEATURES             Location/Qualifiers
     source          1..8389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          318..793
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(4698..4714)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4699..4715)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4808..5202
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(5536..5554)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5622..5726
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5727..5795,5796..6587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5945..5964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6758..7346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7247..7266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8010..8389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctg
      301 atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc ccttcttttc
      361 tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata ttgaccaaat
      421 cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata tacttttttg
      481 tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg
      541 tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat
      601 agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc
      661 atattgctaa tagcagctac aatccagcta ccattctgct tttattttat ggttgggata
      721 aggctggatt attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat
      781 cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa
      841 agaattccgc gggcggccgc catgttcgtg ttcctggtgc tgctgcctct ggtgtccagc
      901 cagtgtgtga acctgaccac cagaacacag ctgcctccag cctacaccaa cagctttacc
      961 agaggcgtgt actaccccga caaggtgttc agatccagcg tgctgcactc tacccaggac
     1021 ctgttcctgc ctttcttcag caacgtgacc tggttccacg ccatccacgt gtccggcacc
     1081 aatggcacca agagattcga caaccccgtg ctgcccttca acgacggggt gtactttgcc
     1141 agcaccgaga agtccaacat catcagaggc tggatcttcg gcaccacact ggacagcaag
     1201 acccagagcc tgctgatcgt gaacaacgcc accaacgtgg tcatcaaagt gtgcgagttc
     1261 cagttctgca acgacccctt cctgggcgtc tactaccaca agaacaacaa gagctggatg
     1321 gaaagcgagt tccgggtgta cagcagcgcc aacaactgca ccttcgagta cgtgtcccag
     1381 cctttcctga tggacctgga aggcaagcag ggcaacttca agaacctgcg cgagttcgtg
     1441 tttaagaaca tcgacggcta cttcaaaatc tacagcaagc acacccctat caacctcgtg
     1501 cgggatctgc ctcagggctt ctctgctctg gaacccctgg tggatctgcc catcggcatc
     1561 aacatcaccc ggtttcagac actgctggcc ctgcacagaa gctacctgac acctggcgat
     1621 agcagcagcg gatggacagc tggtgccgcc gcttactatg tgggctacct gcagcctaga
     1681 accttcctgc tgaagtacaa cgagaacggc accatcaccg acgccgtgga ttgtgctctg
     1741 gaccctctga gcgagacaaa gtgcaccctg aagtccttca ccgtggaaaa gggcatctac
     1801 cagaccagca acttccgggt gcagcccacc gaatccatcg tgcggttccc caatatcacc
     1861 aatctgtgcc ccttcggcga ggtgttcaat gccaccagat tcgcctctgt gtacgcctgg
     1921 aaccggaagc ggatcagcaa ttgcgtggcc gactactccg tgctgtacaa ctccgccagc
     1981 ttcagcacct tcaagtgcta cggcgtgtcc cctaccaagc tgaacgacct gtgcttcaca
     2041 aacgtgtacg ccgacagctt cgtgatccgg ggagatgaag tgcggcagat tgcccctgga
     2101 cagacaggca agatcgccga ctacaactac aagctgcccg acgacttcac cggctgtgtg
     2161 attgcctgga acagcaacaa cctggactcc aaagtcggcg gcaactacaa ttacctgtac
     2221 cggctgttcc ggaagtccaa tctgaagccc ttcgagcggg acatcagcac cgaaatctat
     2281 caggccggca gcaccccttg caacggcgtg gaaggcttca actgctactt cccactgcag
     2341 tcctacggct ttcagcccac aaatggcgtg ggctatcagc cctacagagt ggtggtgctg
     2401 agcttcgaac tgctgcatgc ccctgccaca gtgtgcggcc ctaagaaaag caccaatctc
     2461 gtgaagaaca aatgcgtgaa cttcaacttc aacggcctga ccggcaccgg cgtgctgaca
     2521 gagagcaaca agaagttcct gccattccag cagtttggcc gggatatcgc cgataccaca
     2581 gacgccgtta gagatcccca gacactggaa atcctggaca tcaccccatg cagcttcggc
     2641 ggagtgtctg tgatcacccc tggcaccaac accagcaatc aggtggcagt gctgtaccag
     2701 gacgtgaact gtaccgaagt gcccgtggcc attcacgccg atcagctgac acctacatgg
     2761 cgggtgtact ccaccggcag caatgtgttt cagaccagag ccggctgtct gatcggagcc
     2821 gagcacgtga acaatagcta cgagtgcgac atccccatcg gcgctggcat ctgtgccagc
     2881 taccagacac agacaaacag ccccagacgg gctagaagcg tggccagcca gagcatcatt
     2941 gcctacacaa tgtctctggg cgccgagaac agcgtggcct actccaacaa ctctatcgct
     3001 atccccacca acttcaccat cagcgtgacc accgagatcc tgcctgtgtc catgaccaag
     3061 accagcgtgg actgcaccat gtacatctgc ggcgattcca ccgagtgctc caacctgctg
     3121 ctgcagtacg gcagcttctg cacccagctg aatagagccc tgacagggat cgccgtggaa
     3181 caggacaaga acacccaaga ggtgttcgcc caagtgaagc aaatctacaa gacccctcct
     3241 atcaaggact tcggcggctt caatttcagc cagattctgc ccgatcctag caagcccagc
     3301 aagcggagct tcatcgagga cctgctgttc aacaaagtga cactggccga cgccggcttc
     3361 atcaagcagt atggcgattg tctgggcgac attgccgcca gggatctgat ttgcgcccag
     3421 aagtttaacg gactgacagt gctgcctcct ctgctgaccg atgagatgat cgcccagtac
     3481 acatctgccc tgctggccgg cacaatcaca agcggctgga catttggagc tggcgccgct
     3541 ctgcagatcc cctttgctat gcagatggcc taccggttca acggcatcgg agtgacccag
     3601 aatgtgctgt acgagaacca gaagctgatc gccaaccagt tcaacagcgc catcggcaag
     3661 atccaggaca gcctgagcag cacagcaagc gccctgggaa agctgcagga cgtggtcaac
     3721 cagaatgccc aggcactgaa caccctggtc aagcagctgt cctccaactt cggcgccatc
     3781 agctctgtgc tgaacgatat cctgagcaga ctggacaagg tggaagccga ggtgcagatc
     3841 gacagactga tcaccggaag gctgcagtcc ctgcagacct acgttaccca gcagctgatc
     3901 agagccgccg agattagagc ctctgccaat ctggccgcca ccaagatgtc tgagtgtgtg
     3961 ctgggccaga gcaagagagt ggacttttgc ggcaagggct accacctgat gagcttccct
     4021 cagtctgccc ctcacggcgt ggtgtttctg cacgtgacat acgtgcccgc tcaagagaag
     4081 aatttcacca ccgctccagc catctgccac gacggcaaag cccactttcc tagagaaggc
     4141 gtgttcgtgt ccaacggcac ccattggttc gtgacccagc ggaacttcta cgagccccag
     4201 atcatcacca ccgacaacac cttcgtgtct ggcaactgcg acgtcgtgat cggcattgtg
     4261 aacaataccg tgtacgaccc tctgcagccc gagctggaca gcttcaaaga ggaactggat
     4321 aagtacttta agaaccacac aagccccgac gtggacctgg gcgatatcag cggaatcaat
     4381 gccagcgtcg tgaacatcca gaaagagatc gaccggctga acgaggtggc caagaatctg
     4441 aacgagagcc tgatcgacct gcaagaactg gggaagtacg agcagtacat caagtggccc
     4501 tggtacatct ggctgggctt tatcgccgga ctgattgcca tcgtgatggt cacaatcatg
     4561 ctgtgttgca tgaccagctg ctgtagctgc ctgaagggct gttgctcttg cggctcttgc
     4621 tgcaaattcg atgaggacga ttccgaacct gtcttgaagg gtgtcaagct ccattacaca
     4681 taaggatcca agcttatcga taccgtcgac ctcgagggcc cagatctaat tcaccccacc
     4741 agtgcaggct gcctatcaga aagtggtggc tggtgtggct aatgccctgg cccacaagta
     4801 tcactaagct cgctttcttg ctgtccaatt tctattaaag gttcctttgt tccctaagtc
     4861 caactactaa actgggggat attatgaagg gccttgagca tctggattct gcctaataaa
     4921 aaacatttat tttcattgca atgatgtatt taaattattt ctgaatattt tactaaaaag
     4981 ggaatgtggg aggtcagtgc atttaaaaca taaagaaatg aagagctagt tcaaaccttg
     5041 ggaaaataca ctatatctta aactccatga aagaaggtga ggctgcaaac agctaatgca
     5101 cattggcaac agcccctgat gcctatgcct tattcatccc tcagaaaagg attcaagtag
     5161 aggcttgatt tggaggttaa agttttgcta tgctgtattt tacattactt attgttttag
     5221 ctgtcctcat gaatgtcttt tcactaccca tttgcttatc ctgcatctct cagccttgac
     5281 tccactcagt tctcttgctt agagatacca cctttcccct gaagtgttcc ttccatgttt
     5341 tacggcgaga tggtttctcc tcgcctggcc actcagcctt agttgtctct gttgtcttat
     5401 agaggtctac ttgaagaagg aaaaacaggg ggcatggttt gactgtcctg tgagcccttc
     5461 ttccctgcct cccccactca cagtgacccg gaatccctcg acatggcagt ctagatcatt
     5521 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5581 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5641 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5701 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5761 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5821 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5881 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5941 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6001 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6061 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6121 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6181 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6241 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6301 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6361 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6421 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6481 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6541 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6601 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6661 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6721 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6781 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6841 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6901 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6961 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7021 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7081 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7141 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7201 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7261 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7321 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgg agatgcgccg cgtgcggctg
     7381 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
     7441 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
     7501 cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc
     7561 agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg
     7621 cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg
     7681 cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     7741 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     7801 agcctcgcgt cggggagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     7861 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     7921 cagccatgag cttggcccat tgcatacgtt gtatccatat cataatatgt acatttatat
     7981 tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta
     8041 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     8101 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     8161 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     8221 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     8281 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     8341 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatg
//
