LOCUS       sequence-32294-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-K112503
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..58
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(88..105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(167..191)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(341..360)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(425..444)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggatgattt ctggaattca tgagatctga acaaaaactc atctcagaag aggatctgta
       61 aggatcctaa ctcgacgtgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
      121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaatc aattcgaccc agctttcttg
      181 tacaaagtgg ttgatcggat ctgctgatcc ttcaactcag caaaagttcg atttattcaa
      241 caaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
      301 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
      361 attcaacggg aaacgtcttg ctccaggccg cgattaaatt ccaacatgga tgctgattta
      421 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg
      481 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
      541 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
      601 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg
      661 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
      721 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
      781 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
      841 agtgattt
//
