LOCUS       sequence-32295-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72032
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..72
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(102..119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(181..205)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(355..374)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(439..458)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggatctgaa gtggattcat gagatctaaa gaaaccgctg ctgctaaatt cgaacgtcag
       61 cacatggatt cctaaggatc ctaactcgct cctcaggctt cctcgctcac tgactcgctg
      121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatcaattcg
      181 acccagcttt cttgtacaaa gtggttgatc ggatctgctg atccttcaac tcagcaaaag
      241 ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag
      301 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
      361 gtgttatgag ccatattcaa cgggaaacgt cttgctccag gccgcgatta aattccaaca
      421 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
      481 caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
      541 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
      601 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
      661 ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
      721 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
      781 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
//
