LOCUS       sequence-32296-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72033
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..54
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(84..101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(163..187)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(337..356)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(421..440)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggatctgaa gtggattcat gagatctacc tggagccacc cgcagttcga aaaataagga
       61 tcctaactcg ctcctcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      121 cggcgagcgg tatcagctca ctcaaaggcg gtaatcaatt cgacccagct ttcttgtaca
      181 aagtggttga tcggatctgc tgatccttca actcagcaaa agttcgattt attcaacaaa
      241 gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
      301 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
      361 aacgggaaac gtcttgctcc aggccgcgat taaattccaa catggatgct gatttatatg
      421 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
      481 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      541 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      601 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa
      661 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      721 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      781 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      841 attt
//
