LOCUS       sequence-32298-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72035
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..65
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(95..112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(174..198)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(348..367)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(432..451)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctgaagtgga attcatgaga tctggtaagc ctatccctaa ccctctgctg ggtctcgatt
       61 ctacgtaagg atcctaactc gctcctcagg cttcctcgct cactgactcg ctgcgctcgg
      121 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatcaat tcgacccagc
      181 tttcttgtac aaagtggttg atcggatctg ctgatccttc aactcagcaa aagttcgatt
      241 tattcaacaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
      301 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
      361 gagccatatt caacgggaaa cgtcttgctc caggccgcga ttaaattcca acatggatgc
      421 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
      481 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
      541 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
      601 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
      661 ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
      721 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
      781 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
      841 tgatgcgagt gat
//
