LOCUS       sequence-32299-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72036
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..61
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     primer_bind     complement(91..108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(170..194)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(344..363)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(428..447)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggatctgaa gtggaattca tgagatctta caccgatata gagatgaaca ggctgggaaa
       61 gtaaggatcc taactcgctc ctcaggcttc ctcgctcact gactcgctgc gctcggtcgt
      121 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atcaattcga cccagctttc
      181 ttgtacaaag tggttgatcg gatctgctga tccttcaact cagcaaaagt tcgatttatt
      241 caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
      301 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
      361 catattcaac gggaaacgtc ttgctccagg ccgcgattaa attccaacat ggatgctgat
      421 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
      481 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
      541 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
      601 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
      661 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
      721 gcgctggcag tgttcctgcg ccgg
//
