LOCUS       sequence-32300-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72037
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    204..228
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     290..307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(736..760)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
       61 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
      121 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
      181 tgaaggatca gcagatccga tcaaccactt tgtacaagaa agctgggtcg aattgattac
      241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      301 gagcgaggaa gcctgaggag cgagttagga tccttaagag gtcagtttgt cgtgaacctg
      361 tttgcaaacc tgttcgattt cacggatttc accacgtttg tattccagct gttcttccag
      421 cagcatgatc agagattcac gagactggtg cggacggtat tcgttcagca ggtggtggat
      481 gttaaccagg atggtacgga tgttttcaac tttacgttcg tacatgtccg ggttgataga
      541 cagaacaccg atcagttcca ggtagttcag cagcagagat ttcagcagtt tacgcagttc
      601 ctggattttg tactggtagt tggtagattc gttttcggta gattttttca tagatctcat
      661 gaattccact tcagatcctt agcgaaagct aaggattttt tttatctgaa attctgcctc
      721 gtgataccaa ttcggagcct gcttttttgt acaaacttgt tgataatggc aattcttgat
      781 cgggagatcc gtgattcgca cgggcccatg gctaattccc atgtcagccg ttaagtgttc
      841 ctgt
//
