LOCUS       sequence-32302-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLKA-J72039
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(174..278)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    325..349
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     411..428
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
       61 aactgatcct ctgcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
      121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      301 cctgacgtct aagagatccg atcaaccact ttgtacaaga aagctgggtc gaattgatta
      361 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      421 tgagcgagga agcctgagga gcgagttagg atccagaggt cagtttgtcg tgaacctgtt
      481 tgcaaacctg ttcgatttca cggatttcac cacgtttgta ttccagctgt tcttccagca
      541 gcatgatcag agattcacga gactggtgcg gacggtattc gttcagcagg tggtggatgt
      601 taaccaggat ggtacggatg ttttcaactt tacgttcgta catgtccggg ttgatagaca
      661 gaacaccgat cagttccagg tagttcagca gcagagattt cagcagttta cgcagttcct
      721 ggattttgta ctggtagttg gtagattcgt tttcggtaga ttttttcatc gctatggagt
      781 gccttaatac gttgatttta gctgagatct catg
//
