LOCUS       sequence-323021-        3288 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of your protein of interest fused with TurboID and 
            mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search
            for client proteins within light-induced biomolecular condensates..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Frattini C, Promonet A, Alghoul E, Vidal-Eychenie S, Lamarque M, 
            Blanchard MP, Urbach S, Basbous J, Constantinou A
  TITLE     TopBP1 assembles nuclear condensates to switch on ATR signaling.
  JOURNAL   Mol Cell. 2021 Jan 16. pii: S1097-2765(20)30991-6. doi: 
            10.1016/j.molcel.2020.12.049.
  PUBMED    33503405
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 16. pii: S1097-2765(20)30991-6. doi: 
            10.1016/j.molcel.2020.12.049."
COMMENT     Sequence Label: pCDNA5_FRT_TO_TurboID-mCherry-Cry2
FEATURES             Location/Qualifiers
     source          1..3288
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..960
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             1705..3198
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
ORIGIN
        1 atgaaagaca atactgtgcc tctgaagctg atcgctctcc tggctaatgg cgagttccat
       61 agtggcgaac agctgggaga aaccctgggc atgtccaggg ccgctatcaa caagcacatt
      121 cagactctgc gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg ctactctctg
      181 cccgagccta tcccgctgct gaacgctaaa cagattctgg gacagctgga cggcgggagc
      241 gtggcagtcc tgcctgtggt cgactccacc aatcagtacc tgctggatcg aatcggcgag
      301 ctgaagagtg gggatgcttg cattgcagaa tatcagcagg cagggagagg aagcagaggg
      361 aggaaatggt tctctccttt tggagctaac ctgtacctga gtatgttttg gcgcctgaag
      421 cggggaccag cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc agaagcgctg
      481 cgaaagctgg gagcagacaa ggtgcgagtc aaatggccca atgacctgta tctgcaggat
      541 agaaagctgg caggcatcct ggtggagctg gccggaataa caggcgatgc tgcacagatc
      601 gtcattggcg ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt ggtcaatcag
      661 ggctggatca cactgcagga agcagggatt aacctggaca ggaatactct ggccgctacg
      721 ctgatccgag agctgcgggc agccctggaa ctgttcgagc aggaaggcct ggctccatat
      781 ctgccacggt gggagaagct ggataacttc atcaatagac ccgtgaagct gatcattggg
      841 gacaaagaga ttttcgggat tagccggggg attgataaac agggagccct gctgctggaa
      901 caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag
      961 cacgtgggta ccatcgatgc tagcatggtg tctaaaggcg aggaggataa catggctata
     1021 atcaaggaat ttatgaggtt caaggtgcat atggagggat ctgtgaacgg tcacgagttc
     1081 gaaatcgagg gcgaaggcga ggggcgaccc tatgaaggga cacagacggc caaactgaag
     1141 gtgaccaagg gtggccccct gcctttcgcc tgggacatcc tgtcacccca gtttatgtac
     1201 ggctctaagg cttacgttaa gcaccctgcc gatattccgg actacttgaa actgagcttc
     1261 cctgaagggt tcaagtggga acgggtaatg aattttgagg atggcggtgt agtcacagtt
     1321 acccaggaca gctctcttca ggacggagaa tttatctata aggttaaact tcggggcact
     1381 aacttcccat cagacggccc cgtgatgcag aagaaaacta tgggctggga agccagctca
     1441 gaacgcatgt atcccgagga cggagcactg aagggagaaa tcaagcagcg actcaagctg
     1501 aaggatgggg gacattatga tgctgaggtc aaaaccacct ataaggccaa gaaacccgtt
     1561 cagcttcctg gggcatacaa cgtgaatatc aaactggata ttacatccca taacgaagac
     1621 tataccatcg tggaacagta cgagcgggcc gagggcagac atagcacagg gggcatggat
     1681 gaattgtaca aggcggccac gcgtatgaag atggacaaaa agactatagt ttggtttaga
     1741 agagacctaa ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt
     1801 tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca
     1861 agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct
     1921 gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt
     1981 accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac
     2041 cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat
     2101 ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat
     2161 tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg
     2221 cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta
     2281 gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca
     2341 ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat
     2401 gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat
     2461 ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg
     2521 gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt
     2581 ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg
     2641 ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga
     2701 caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga
     2761 tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt
     2821 ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt
     2881 gacatccttg gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc
     2941 ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa
     3001 tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct
     3061 ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta
     3121 gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag
     3181 atcatgatcg gagcagcagc ccgggatcca ccggtcgcca ccggttcagg gagcggatcc
     3241 ggctcaggga gtgcggccgc aactcccacc tgcaacatgc gtgactga
//
