LOCUS       sequence-323033-       14473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multigene construct containing the betalain pigment biosynthetic 
            genes under the control of the NbBCP1b promoter for visualisation of
            arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14473)
  AUTHORS   Timoneda A, Yunusov T, Quan C, Gavrin A, Brockington SF, Schornack S
  TITLE     MycoRed: Betalain pigments enable in vivo real-time visualisation of
            arbuscular mycorrhizal colonisation.
  JOURNAL   PLoS Biol. 2021 Jul 14;19(7):e3001326. doi: 
            10.1371/journal.pbio.3001326.
  PUBMED    34260583
REFERENCE   2  (bases 1 to 14473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2021-07-14"; volume: "19(7):e3001326. doi"; pages: " 
            10.1371/journal.pbio.3001326"
COMMENT     Sequence Label: NbBCP1b-p3
FEATURES             Location/Qualifiers
     source          1..14473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    36..60
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             369..998
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1427..2500
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2566..2760
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        2822..2926
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2927..3721
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3892..4480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4381..4400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4616..4640
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(4748..5000)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(5600..5782)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(5636..5814)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      7985..8237
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    14272..14446
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
       61 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
      121 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga gtcagccgtg
      181 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
      241 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
      301 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
      361 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
      421 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
      481 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
      541 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
      601 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
      661 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
      721 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
      781 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
      841 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
      901 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
      961 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     1021 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     1081 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     1141 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     1201 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     1261 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     1321 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     1381 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     1441 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     1501 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     1561 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgtc
     1621 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     1681 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     1741 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     1801 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     1861 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     1921 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     1981 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     2041 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     2101 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     2161 agctgattgg atgtaccgcg agattacaga aggcaagaac ccggacgtgc tgacggttca
     2221 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     2281 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     2341 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     2401 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     2461 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     2521 gcaaattgcc ctagcagggg aaaaaggtcg aaaaagcttc tttcctgtgg atagcacgta
     2581 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     2641 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     2701 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     2761 actgtctggc cagcgcacag ccgaacagct gcaaaaagcg cccacatcaa ggctccgagt
     2821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag
     2941 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
     3001 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
     3061 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
     3121 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
     3181 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
     3241 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
     3301 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
     3361 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagagg acactccatt
     3421 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
     3481 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
     3541 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
     3601 ccggtcgctc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
     3661 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
     3721 gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3781 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3841 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4021 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4501 tttacggttc ctgctcggat ctgttggacc ggacagtagt catggttgat gggctgcctg
     4561 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
     4621 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
     4681 ttttaatgta ctggggttga acactctgtg ccgaattcgg atccagcgct ccaattcagc
     4741 aagcgtcgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
     4801 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
     4861 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
     4921 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
     4981 tgccaaatgt ttgaacgatc tgcttgactc tagatccaga gtcccgctca aatctcagtc
     5041 acaggaagaa caggtctagg aggaacagga agagagaaat caagctgcca gaatcccaca
     5101 tcatgccaat ttccatgttt gaaaccagca gctctaagca ttcccctagg agcataacca
     5161 agagcctcat gcatcctcac agaaggatca ttaggaagtc caatcacagc aaccacagac
     5221 ttgaaaccct gagcctccaa agacttcaaa agatgagtgt aaagagtaga tccaagacca
     5281 gttctttgat gcctaggaga cacgtaaaca gtagactcag cagtccaatc gtaagcattc
     5341 ctagccttcc aaggaccagc ataagcaata ccagccactt caccatccac ttcagcaaca
     5401 agccaaggat acctctctct aagcctcaca agatcatcag tccactcttg tggttcttga
     5461 ggctcagtcc tgaaattcac agtagaagtc tcaatgtaat gattcacaat agtgcacaca
     5521 gcaggcatat cagcctcagt agcccttcta atatcagcag gccttctttc aggagacatg
     5581 cgaaacgatc cagatccggt gcagattatt tggattgaga gtgaatatga gactctaatt
     5641 ggataccgag gggaatttat ggaacgtcag tggagcattt tgacaagaaa tatttgctag
     5701 ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta
     5761 gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag
     5821 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
     5881 attctccgct ccgcaagaat tcaagcttgg agactggtat tagaataata gcctgtttag
     5941 ccaaaaattg aagtgtttgg ttttggaagg aaaaaaaagt attttgagga gaggcagaag
     6001 cagaaaaaag tagcttctct ccaaaagtac ttttttgaga agcatttttg agaaaaatac
     6061 acttagaagc agttttttaa agcttggcca atcactaatt gctgctcaga agtgcttttc
     6121 aaactaatta gccaaacaca aactacttct catcaaaagt actttcgaaa aaaacactta
     6181 ttaaagtaag ctaatttttg cagcttggct aaatggatta taaatcacgt atctgtaaaa
     6241 ctacaaatta tattttattg ttcctcacat ttttcgaggt aaacataaat ataagtaaaa
     6301 tataatggta atgtactcca cttgctgaat aacttagtgt ttcatagcaa gcatatgctc
     6361 cacaaattaa gagtatggag ccataatatt tccaatatga gtgaccctat agttacaata
     6421 acaacaacaa tccagtataa tctcacatag tggggtctgg tgagggtagt atgtacgtag
     6481 accttatctc tatcctgggg tagagaggct gtttccaaat agactcccga catcattctc
     6541 tccaagaact tctcaccttg ctcttgggga aactcgaact cacaacctct atagttaaaa
     6601 catgataaat tatggtcata tttgtattct ctttttttgc tttaattttg ggtatgataa
     6661 aattccttat aaatgctaac aacctactcc gtcctgttcc aaatagtaat cgttttagca
     6721 tgctgaaatt gtttcaaaat atttggcatt ttagaaaaat aaaatatatt tgttattttt
     6781 ttattaacct tactactcca attagtcgat tgttaataag atgagacatt taagagaaaa
     6841 aaacaacgat aagttagaca aataatctta taattaaata tgtcaaaaat cataaaagac
     6901 aaataatatg gaatagagta aatatctacc tacaatacta aggttttctt ctcctttgaa
     6961 ctttacgaga ataattagtg gaatatttgc cagcctaact cattggaatt cacgtactag
     7021 aattcaaatt aatgaatcac acttcgttat cctcggatct cgctaagctc tcttgttaag
     7081 ttaaggtata aatagccata ccaatttttc ttttttcaac taacccttaa agaagaattt
     7141 tccaatgaaa atgatgaatg gagaagatgc taatgatcaa atgatcaaag aaagcttctt
     7201 cataacacat ggaaacccaa tattaacagt agaagataca catccattaa gacctttctt
     7261 tgaaacttgg agagagaaaa tcttttctaa gaaacctaag gcaattctta ttatttctgg
     7321 tcattgggaa actgttaaac ctactgttaa tgctgtccat atcaatgata ctatccatga
     7381 ttttgatgac tatcctgctg ctatgtacca gttcaagtat ccagctcctg gggcaccaga
     7441 attggcaaga aaagtagagg aaattctgaa aaaatcgggt ttcgaaacgg cggaaactga
     7501 cgaaaaacgt gggcttgatc atggtgcatg ggtacctcta atgctaatgt atcctgaggc
     7561 tgatatacca gtatgtcagc tctcagttca gccgcattta gatggaacat accattacaa
     7621 cttaggacga gcattagctc ccttgaaaaa cgatggcgta ttgatcattg gttcaggaag
     7681 tgcaactcac cctttggatg aaactcctca ttattttgat ggagttgcac cttgggcagc
     7741 tgcctttgat tcatggcttc gtaaagctct cataaatgga aggtttgaag aagtgaatat
     7801 atatgaaacg aaagcaccaa attggaaatt agcacatcct ttcccagaac atttttatcc
     7861 attgcatgtt gttcttggcg ctgctggtga aaaatggaag gctgagctta ttcatagcag
     7921 ttgggatcat ggcaccttgt gtcacggctc ctacaagttc acttcagcct aggcttgtca
     7981 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     8041 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     8101 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     8161 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     8221 atctatgtta ctagatcgac gctactagaa ttcgagctcg gagactggta ttagaataat
     8281 agcctgttta gccaaaaatt gaagtgtttg gttttggaag gaaaaaaaag tattttgagg
     8341 agaggcagaa gcagaaaaaa gtagcttctc tccaaaagta cttttttgag aagcattttt
     8401 gagaaaaata cacttagaag cagtttttta aagcttggcc aatcactaat tgctgctcag
     8461 aagtgctttt caaactaatt agccaaacac aaactacttc tcatcaaaag tactttcgaa
     8521 aaaaacactt attaaagtaa gctaattttt gcagcttggc taaatggatt ataaatcacg
     8581 tatctgtaaa actacaaatt atattttatt gttcctcaca tttttcgagg taaacataaa
     8641 tataagtaaa atataatggt aatgtactcc acttgctgaa taacttagtg tttcatagca
     8701 agcatatgct ccacaaatta agagtatgga gccataatat ttccaatatg agtgacccta
     8761 tagttacaat aacaacaaca atccagtata atctcacata gtggggtctg gtgagggtag
     8821 tatgtacgta gaccttatct ctatcctggg gtagagaggc tgtttccaaa tagactcccg
     8881 acatcattct ctccaagaac ttctcacctt gctcttgggg aaactcgaac tcacaacctc
     8941 tatagttaaa acatgataaa ttatggtcat atttgtattc tctttttttg ctttaatttt
     9001 gggtatgata aaattcctta taaatgctaa caacctactc cgtcctgttc caaatagtaa
     9061 tcgttttagc atgctgaaat tgtttcaaaa tatttggcat tttagaaaaa taaaatatat
     9121 ttgttatttt tttattaacc ttactactcc aattagtcga ttgttaataa gatgagacat
     9181 ttaagagaaa aaaacaacga taagttagac aaataatctt ataattaaat atgtcaaaaa
     9241 tcataaaaga caaataatat ggaatagagt aaatatctac ctacaatact aaggttttct
     9301 tctcctttga actttacgag aataattagt ggaatatttg ccagcctaac tcattggaat
     9361 tcacgtacta gaattcaaat taatgaatca cacttcgtta tcctcggatc tcgctaagct
     9421 ctcttgttaa gttaaggtat aaatagccat accaattttt cttttttcaa ctaaccctta
     9481 aagaagaatt ttccaatgga tcatgcaaca ttagcaatga tactagccat ttggttcatt
     9541 tcttttcatt tcataaaatt actttttagc caacaaacta ccaaacttct tcctcctggt
     9601 ccaaaaccat tgccaataat tggcaacatc ttagaagttg gtaaaaaacc tcatcgttca
     9661 tttgctaatc ttgctaaaat tcacggccct ttaatatcgt tacgtctagg aagtgtaaca
     9721 actattgttg tatcatcagc agatgtagct aaagaaatgt tcttaaaaaa agaccaccct
     9781 ctttctaacc gtactattcc taattctgtc acggccggtg accaccataa acttaccatg
     9841 tcgtggttgc cggtttcgcc gaaatggcgg aattttcgta agattacagc cgtccatttg
     9901 ctttctcctc aaagacttga tgcttgccaa acctttcgcc atgcgaaggt gcaacagctt
     9961 tatgaatatg tgcaagaatg tgcacaaaaa ggccaagctg ttgatattgg caaagctgca
    10021 tttactacct cccttaattt gttatctaaa ctattttttt cagtggaatt agcccaccat
    10081 aaatcacaaa cttctcaaga gtttaaggaa ctaatatgga acattatgga agatattggg
    10141 aaacctaact atgctgatta ttttcctatt ttaggctgtg ttgatccatc aggtattcga
    10201 cgaagattag catgtagttt tgacaagttg attgcagttt ttcagagtat aatatgtgaa
    10261 aggcttgcgc ctgattcttc aactgcaaca acaacgacga ctgatgatgt gctagacgtt
    10321 cttcttcagc tcttcaaaca aaatgagctc actatgggcg agattaacca tttgctcgtc
    10381 gacatttttg atgctggaac agacactaca tcaagtactt ttgagtgggt catggcagag
    10441 ttaattagga atcctgaaat gatggaaaag gctcaagaag aaattaagca agtattaggc
    10501 aaagataaac aaattcagga atcagacatt attaacctac cttacttaca agccattatc
    10561 aaagaaactt tgcgactaca tccaccaact gtatttcttt tgcctcgtaa agccgacact
    10621 gatgttgaac tatatggtta tattgtgcct aaagatgcac aaatacttgt taacttatgg
    10681 gctattggaa gagatcctaa tgcctggcaa aatgctgata ttttttcgcc cgaaagattt
    10741 atagggtgtg aaattgatgt caaaggaaga gattttggac tcttaccttt tggagctgga
    10801 agaaggatat gtcctgggat gaatttggcc attagaatgt taactttgat gctagctact
    10861 ttgcttcaat tcttcaattg gaagcttgaa ggagacataa gtccaaaaga cttagacatg
    10921 gatgagaaat ttgggattgc gttacaaaag acaaagcctt taaaacttat tccaatacct
    10981 aggtattgag cttgctctca agatcaaagg cttaaaaagc tggggtttta tgaatgggat
    11041 caaagtttct ttttttcttt tatatttgct tctccatttg tttgtttcat ttcccttttt
    11101 gttttcgttt ctatgatgca cttgtgtgtg acaaactctc tgggttttta cttacgtctg
    11161 cgtttcaaaa aaaaaaaccg ctttcgtttt gcgttttagt cccattgttt tgtagctctg
    11221 agtgatcgaa ttgatgcctc tttattcctt ttgttcccta taatttcttt caaaactcag
    11281 aagaaaaacc ttgaaactct ttgcaatgtt aatataagta ttgtataaga tttttattga
    11341 tttggttatt agtcttactt ttgctacctc catcttcact tggaactgat attctgaata
    11401 gttaaagcgt tacatgtgtt ccattcacaa atgaacttaa actagcacaa agtcagatat
    11461 tttaagatcg caccatttcg ctttacgaat tcccatgggg agactggtat tagaataata
    11521 gcctgtttag ccaaaaattg aagtgtttgg ttttggaagg aaaaaaaagt attttgagga
    11581 gaggcagaag cagaaaaaag tagcttctct ccaaaagtac ttttttgaga agcatttttg
    11641 agaaaaatac acttagaagc agttttttaa agcttggcca atcactaatt gctgctcaga
    11701 agtgcttttc aaactaatta gccaaacaca aactacttct catcaaaagt actttcgaaa
    11761 aaaacactta ttaaagtaag ctaatttttg cagcttggct aaatggatta taaatcacgt
    11821 atctgtaaaa ctacaaatta tattttattg ttcctcacat ttttcgaggt aaacataaat
    11881 ataagtaaaa tataatggta atgtactcca cttgctgaat aacttagtgt ttcatagcaa
    11941 gcatatgctc cacaaattaa gagtatggag ccataatatt tccaatatga gtgaccctat
    12001 agttacaata acaacaacaa tccagtataa tctcacatag tggggtctgg tgagggtagt
    12061 atgtacgtag accttatctc tatcctgggg tagagaggct gtttccaaat agactcccga
    12121 catcattctc tccaagaact tctcaccttg ctcttgggga aactcgaact cacaacctct
    12181 atagttaaaa catgataaat tatggtcata tttgtattct ctttttttgc tttaattttg
    12241 ggtatgataa aattccttat aaatgctaac aacctactcc gtcctgttcc aaatagtaat
    12301 cgttttagca tgctgaaatt gtttcaaaat atttggcatt ttagaaaaat aaaatatatt
    12361 tgttattttt ttattaacct tactactcca attagtcgat tgttaataag atgagacatt
    12421 taagagaaaa aaacaacgat aagttagaca aataatctta taattaaata tgtcaaaaat
    12481 cataaaagac aaataatatg gaatagagta aatatctacc tacaatacta aggttttctt
    12541 ctcctttgaa ctttacgaga ataattagtg gaatatttgc cagcctaact cattggaatt
    12601 cacgtactag aattcaaatt aatgaatcac acttcgttat cctcggatct cgctaagctc
    12661 tcttgttaag ttaaggtata aatagccata ccaatttttc ttttttcaac taacccttaa
    12721 agaagaattt tccaatgacc gccattaaaa tgaacaccaa tggtgaaggt gaaacacaac
    12781 atatactaat gatacctttc atggcgcaag ggcatttacg tcctttcctt gagcttgcta
    12841 tgtttctata taaacgaagt cattttatca ttactcttct tactaccccg ctcaatgcgg
    12901 gtttcctacg acatctcctt caccaccata gctattctag ctcggggatc agaattgtcg
    12961 agttaccttt caactcaacc aatcatggac ttccacctgg cattgaaaac actgataaac
    13021 ttacactccc acttgtagta tcactttttc attcaaccat ttctcttgac cctcacctta
    13081 gagattatat ttcccgccat ttctcccctg cgcgccctcc tctgtgtgtc atacatgatg
    13141 tgttccttgg ttgggttgat caagttgcta aagacgtggg ctcaactggt gttgttttta
    13201 ctacgggtgg cgcgtatggt acaagcgcat atgtgtccat ttggaatgat ctgcctcacc
    13261 agaattactc tgatgatcaa gagtttccgc ttcctggttt cccggagaat cataaattcc
    13321 gacgttctca acttcatcgg tttctgaggt atgccgatgg atcagatgat tggtcgaaat
    13381 atttccaacc gcaattgagg caatcaatga agagttttgg atggctatgt aattcagttg
    13441 aggaaatcga aacacttggg tttagtatcc tcaggaacta cactaaacta cccatttggg
    13501 gtattggacc gttgatagct tcacctgtac aacattcatc atctgataat aacagtactg
    13561 gtgccgagtt tgttcaatgg ttgagcttga aagaaccaga ttctgtatta tacatctcat
    13621 ttggatcaca gaacacaatt tcaccaaccc agatgatgga actagcagcc ggtttggagt
    13681 caagtgagaa gccgtttttg tgggtgattc gagcaccatt tgggttcgat atcaatgagg
    13741 aaatgaggcc agaatggcta ccagagggat tcgaggagcg aatgaaggtg aaaaaacaag
    13801 gaaagttggt gtataagttg ggaccacagt tggagatact taaccatgag tcaatcggag
    13861 ggttcttaac tcattgtggg tggaattcga tccttgagtc acttcgagaa ggtgtgccta
    13921 tgttagggtg gccattggca gccgaacaag cttataattt gaagtatttg gaggacgaaa
    13981 tgggggttgc agtcgagtta gcgaggggat tggaaggaga gataagtaaa gagaaagtga
    14041 agagaattgt ggagatgatt ttagagagaa atgaaggaag taaaggatgg gaaatgaaaa
    14101 atagagcagt agaaatgggg aagaaactta aagacgctgt caatgaggag aaggaactga
    14161 agggttcttc tgttaaggca atagatgatt tcttagatgc ggtcatgcaa gctaagttgg
    14221 aaccttctct tcaataagct tctctagcta gagtcgatcg acaagctcga gtttctccat
    14281 aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg
    14341 tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata
    14401 aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatcgctc agagaggatg
    14461 cacatgtgac cga
//
