LOCUS       sequence-323050-       14585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multigene construct containing the betalain pigment biosynthetic 
            genes under the control of the MtPT4 promoter for visualisation of 
            arbuscular mycorrhizal colonisation in Medicago truncatula roots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14585)
  AUTHORS   Timoneda A, Yunusov T, Quan C, Gavrin A, Brockington SF, Schornack S
  TITLE     MycoRed: Betalain pigments enable in vivo real-time visualisation of
            arbuscular mycorrhizal colonisation.
  JOURNAL   PLoS Biol. 2021 Jul 14;19(7):e3001326. doi: 
            10.1371/journal.pbio.3001326.
  PUBMED    34260583
REFERENCE   2  (bases 1 to 14585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2021-07-14"; volume: "19(7):e3001326. doi"; pages: " 
            10.1371/journal.pbio.3001326"
COMMENT     Sequence Label: MtPT4-p3
FEATURES             Location/Qualifiers
     source          1..14585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(19..271)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      4175..4427
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    9672..9846
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9909..9933
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10242..10871
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11300..12373
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12439..12633
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        12695..12799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12800..13594
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13765..14353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14254..14273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    14489..14513
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaattcggat ccagcgtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
       61 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
      121 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
      181 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
      241 agaaacttta ttgccaaatg tttgaacgat ctgcttgaca agcttaaagg aacagatggt
      301 ggcgtccctc ggttctttca tactgctcaa cgattgtata atcttcgttg tggcttgtta
      361 tatccagttt ggagtcaaca tagtagtacc ctggtagctg cacaggcttc tttgccatgt
      421 aaatactttt gaattcaact aggtaatgac caccgtcttt cagcttcaga gccttatgaa
      481 tctctccttt caacacgcca tcacgaggat acaaacgctc agtgctggct tcccagccca
      541 ttgttttctt ttgcataaca ggtccatcgg aaggaaagtt cacgccaatg aacttgacct
      601 tgtagatgaa acagccatcc tgcaaactgg aatcctgggt tacagtaacg acgccaccat
      661 cttcaaagtt catgaccctt tcccatttaa atccttcagg aaatgacagc tttttatagt
      721 ctggtatgtc ggcagggtgc ttgacatata ccttgcttcc atactgaaat tgtggtgaca
      781 aaatatccca agcaaatggc aaaggtcccc ccttggttac cttaagcttt acggtattgt
      841 ggccttcgta tggcctcccc tctccttcgc cttctatttc aaactcgtgc ccattgaccg
      901 ttccttccat gcgaacctta aacctcatga actccttgat aacattcttg gatgacccca
      961 ttaagatctg catctgttaa tcagaaaaac tcagattaat cgacaaattc gatcgcacaa
     1021 actagaaact aacaccagat ctagatagaa atcacaaatc gaagagtaat tattcgacaa
     1081 aactcaaatt atttgaacaa atcggatgat atctatgaaa ccctaatcga gaattaagat
     1141 gatatctaac gatcaaaccc agaaaatcgt ctacgatcta agattaacag aatctaaacc
     1201 aaagaacata tacgaaattg ggatcgaacg aaaacaaaat cgaagatttt gagagaataa
     1261 ggaacacaga aatttacctt gatcacggta gagagaattg agagaaagtt tttaagattt
     1321 tgagaaattg aaatctgaat tgtgaagaag aagagctctt tgggtattgt tttatagaag
     1381 aagaagaaga aaagacgagg acgactaggt cacgagaaag ctaaggcggt gaagcaatag
     1441 ctaataataa aatgacacgt gtattgagcg ttgtttacac gcaaagttgt ttttggctaa
     1501 ttgccttatt tttaggttga ggaaaagtat ttgtgctttg agttgataaa cacgactcgt
     1561 gtgtgccggc tgcaaccact ttgacgccgt ttattactgg acttcgtccg acaaccacaa
     1621 tttccttaac ggtcgtcata agatccagcc gttgagattt aacgatcgtt acgatttata
     1681 tttttttagc attatcgttt tattttttaa atatacggtg gagctgaaaa ttggcaataa
     1741 ttgaaccgtg ggtcccactg cattgaagcg tatttcgtat tttctagaat tcttcgtgct
     1801 ttatttcttt tcctttttgt ttttttttgc catttatcta atgcaagtgg gcttataaaa
     1861 tcagtgaatt tcttggaaaa gtaacttctt tatcgtataa catattgtga aattatccat
     1921 ttcttttaat ttttttagtg ttattggata tttttgtatg attattgatt tgcataggat
     1981 aatgactttt gtatcaagtt ggtgaacaag tctcgttaaa aaaggcaagt ggtttggtga
     2041 ctcgatttat tcttgttatt taattcatat atcaatggat cttatttggg gcctggtcca
     2101 tatttaacac tcgtgttcag tccaatgacc aataatattt tttcattaat aacaatgtaa
     2161 caagaatgat acacaaaaca ttctttgaat aagttcgcta tgaagaaggg aacttatccg
     2221 gtcctagatc atcagttcat acaaacctcc atagagttca acatcttaaa caagaatatc
     2281 ctgatctgaa gaatgtggag gctttagtcc cttggatact tgggaggctg tggaagaaca
     2341 gaaacgagct ggtgcttaaa gggagggaat ttggaaccaa tgaggtatta gtaaggacac
     2401 aagaagatgc agatgagtgg attagaagga aagaggctca gaatgtaagg aaagcaccaa
     2461 ccacaacgac gtcctccgca agaattcaag cttggagctc gatccacaac aaagattaat
     2521 ttttgttcaa acaactaata ctatctgaaa atacacttcc aaaaatatgt taaatttttg
     2581 ttttttttaa catggagtga aaaaaagttg ctcccttatt atttaattgg cacacatttt
     2641 tgtatgttta gtaaatgaca cgcattctat tcggtggtaa cgtttgtcct cacatacacc
     2701 tcatttgaat tgcatttgta tcctactacc tccctttcca attatataag atatttttga
     2761 attttttttg ttccttttta taagacctcc ttgttatttc caattaaatt aattatttct
     2821 ttacatatat gtccctattt attatacatt tttttcttca atcagcaata aatagcatgt
     2881 tgaaaatata aactatctct ctttctaaag gataaaattg taaaatcaat agtgattaca
     2941 aataacttta atacaaatac tcactttctt aattcctgtg attttggcaa aaaggtcttg
     3001 tatataggga cggaggtagt attatgttag caagcagcta aaaaacaaaa caataatcct
     3061 cttttaacag ctaactgtaa ccaaaacaaa agcacttagc atttttgaac attccctata
     3121 agaatatcct aattaattga tcaacactag gtgaatatcc ataacattta gctagcaatc
     3181 tcaatttttc ttgttctcat ctcctaagct aacttaggac taaacgtcac attgttacat
     3241 tatcaaacac atatcaatgc atagccataa taatatatat aagagcatac acataaacac
     3301 ctaaaccaaa ttaactccaa aaaccaactt gagaatgaaa atgatgaatg gagaagatgc
     3361 taatgatcaa atgatcaaag aaagcttctt cataacacat ggaaacccaa tattaacagt
     3421 agaagataca catccattaa gacctttctt tgaaacttgg agagagaaaa tcttttctaa
     3481 gaaacctaag gcaattctta ttatttctgg tcattgggaa actgttaaac ctactgttaa
     3541 tgctgtccat atcaatgata ctatccatga ttttgatgac tatcctgctg ctatgtacca
     3601 gttcaagtat ccagctcctg gggcaccaga attggcaaga aaagtagagg aaattctgaa
     3661 aaaatcgggt ttcgaaacgg cggaaactga cgaaaaacgt gggcttgatc atggtgcatg
     3721 ggtacctcta atgctaatgt atcctgaggc tgatatacca gtatgtcagc tctcagttca
     3781 gccgcattta gatggaacat accattacaa cttaggacga gcattagctc ccttgaaaaa
     3841 cgatggcgta ttgatcattg gttcaggaag tgcaactcac cctttggatg aaactcctca
     3901 ttattttgat ggagttgcac cttgggcagc tgcctttgat tcatggcttc gtaaagctct
     3961 cataaatgga aggtttgaag aagtgaatat atatgaaacg aaagcaccaa attggaaatt
     4021 agcacatcct ttcccagaac atttttatcc attgcatgtt gttcttggcg ctgctggtga
     4081 aaaatggaag gctgagctta ttcatagcag ttgggatcat ggcaccttgt gtcacggctc
     4141 ctacaagttc acttcagcct aggcttgtca agcagatcgt tcaaacattt ggcaataaag
     4201 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     4261 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     4321 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     4381 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgac gctactagaa
     4441 ttcgagctcg gagctcgatc cacaacaaag attaattttt gttcaaacaa ctaatactat
     4501 ctgaaaatac acttccaaaa atatgttaaa tttttgtttt ttttaacatg gagtgaaaaa
     4561 aagttgctcc cttattattt aattggcaca catttttgta tgtttagtaa atgacacgca
     4621 ttctattcgg tggtaacgtt tgtcctcaca tacacctcat ttgaattgca tttgtatcct
     4681 actacctccc tttccaatta tataagatat ttttgaattt tttttgttcc tttttataag
     4741 acctccttgt tatttccaat taaattaatt atttctttac atatatgtcc ctatttatta
     4801 tacatttttt tcttcaatca gcaataaata gcatgttgaa aatataaact atctctcttt
     4861 ctaaaggata aaattgtaaa atcaatagtg attacaaata actttaatac aaatactcac
     4921 tttcttaatt cctgtgattt tggcaaaaag gtcttgtata tagggacgga ggtagtatta
     4981 tgttagcaag cagctaaaaa acaaaacaat aatcctcttt taacagctaa ctgtaaccaa
     5041 aacaaaagca cttagcattt ttgaacattc cctataagaa tatcctaatt aattgatcaa
     5101 cactaggtga atatccataa catttagcta gcaatctcaa tttttcttgt tctcatctcc
     5161 taagctaact taggactaaa cgtcacattg ttacattatc aaacacatat caatgcatag
     5221 ccataataat atatataaga gcatacacat aaacacctaa accaaattaa ctccaaaaac
     5281 caacttgaga atggatcatg caacattagc aatgatacta gccatttggt tcatttcttt
     5341 tcatttcata aaattacttt ttagccaaca aactaccaaa cttcttcctc ctggtccaaa
     5401 accattgcca ataattggca acatcttaga agttggtaaa aaacctcatc gttcatttgc
     5461 taatcttgct aaaattcacg gccctttaat atcgttacgt ctaggaagtg taacaactat
     5521 tgttgtatca tcagcagatg tagctaaaga aatgttctta aaaaaagacc accctctttc
     5581 taaccgtact attcctaatt ctgtcacggc cggtgaccac cataaactta ccatgtcgtg
     5641 gttgccggtt tcgccgaaat ggcggaattt tcgtaagatt acagccgtcc atttgctttc
     5701 tcctcaaaga cttgatgctt gccaaacctt tcgccatgcg aaggtgcaac agctttatga
     5761 atatgtgcaa gaatgtgcac aaaaaggcca agctgttgat attggcaaag ctgcatttac
     5821 tacctccctt aatttgttat ctaaactatt tttttcagtg gaattagccc accataaatc
     5881 acaaacttct caagagttta aggaactaat atggaacatt atggaagata ttgggaaacc
     5941 taactatgct gattattttc ctattttagg ctgtgttgat ccatcaggta ttcgacgaag
     6001 attagcatgt agttttgaca agttgattgc agtttttcag agtataatat gtgaaaggct
     6061 tgcgcctgat tcttcaactg caacaacaac gacgactgat gatgtgctag acgttcttct
     6121 tcagctcttc aaacaaaatg agctcactat gggcgagatt aaccatttgc tcgtcgacat
     6181 ttttgatgct ggaacagaca ctacatcaag tacttttgag tgggtcatgg cagagttaat
     6241 taggaatcct gaaatgatgg aaaaggctca agaagaaatt aagcaagtat taggcaaaga
     6301 taaacaaatt caggaatcag acattattaa cctaccttac ttacaagcca ttatcaaaga
     6361 aactttgcga ctacatccac caactgtatt tcttttgcct cgtaaagccg acactgatgt
     6421 tgaactatat ggttatattg tgcctaaaga tgcacaaata cttgttaact tatgggctat
     6481 tggaagagat cctaatgcct ggcaaaatgc tgatattttt tcgcccgaaa gatttatagg
     6541 gtgtgaaatt gatgtcaaag gaagagattt tggactctta ccttttggag ctggaagaag
     6601 gatatgtcct gggatgaatt tggccattag aatgttaact ttgatgctag ctactttgct
     6661 tcaattcttc aattggaagc ttgaaggaga cataagtcca aaagacttag acatggatga
     6721 gaaatttggg attgcgttac aaaagacaaa gcctttaaaa cttattccaa tacctaggta
     6781 ttgagcttgc tctcaagatc aaaggcttaa aaagctgggg ttttatgaat gggatcaaag
     6841 tttctttttt tcttttatat ttgcttctcc atttgtttgt ttcatttccc tttttgtttt
     6901 cgtttctatg atgcacttgt gtgtgacaaa ctctctgggt ttttacttac gtctgcgttt
     6961 caaaaaaaaa aaccgctttc gttttgcgtt ttagtcccat tgttttgtag ctctgagtga
     7021 tcgaattgat gcctctttat tccttttgtt ccctataatt tctttcaaaa ctcagaagaa
     7081 aaaccttgaa actctttgca atgttaatat aagtattgta taagattttt attgatttgg
     7141 ttattagtct tacttttgct acctccatct tcacttggaa ctgatattct gaatagttaa
     7201 agcgttacat gtgttccatt cacaaatgaa cttaaactag cacaaagtca gatattttaa
     7261 gatcgcacca tttcgcttta cgaattccca tggggagctc gatccacaac aaagattaat
     7321 ttttgttcaa acaactaata ctatctgaaa atacacttcc aaaaatatgt taaatttttg
     7381 ttttttttaa catggagtga aaaaaagttg ctcccttatt atttaattgg cacacatttt
     7441 tgtatgttta gtaaatgaca cgcattctat tcggtggtaa cgtttgtcct cacatacacc
     7501 tcatttgaat tgcatttgta tcctactacc tccctttcca attatataag atatttttga
     7561 attttttttg ttccttttta taagacctcc ttgttatttc caattaaatt aattatttct
     7621 ttacatatat gtccctattt attatacatt tttttcttca atcagcaata aatagcatgt
     7681 tgaaaatata aactatctct ctttctaaag gataaaattg taaaatcaat agtgattaca
     7741 aataacttta atacaaatac tcactttctt aattcctgtg attttggcaa aaaggtcttg
     7801 tatataggga cggaggtagt attatgttag caagcagcta aaaaacaaaa caataatcct
     7861 cttttaacag ctaactgtaa ccaaaacaaa agcacttagc atttttgaac attccctata
     7921 agaatatcct aattaattga tcaacactag gtgaatatcc ataacattta gctagcaatc
     7981 tcaatttttc ttgttctcat ctcctaagct aacttaggac taaacgtcac attgttacat
     8041 tatcaaacac atatcaatgc atagccataa taatatatat aagagcatac acataaacac
     8101 ctaaaccaaa ttaactccaa aaaccaactt gagaatgacc gccattaaaa tgaacaccaa
     8161 tggtgaaggt gaaacacaac atatactaat gatacctttc atggcgcaag ggcatttacg
     8221 tcctttcctt gagcttgcta tgtttctata taaacgaagt cattttatca ttactcttct
     8281 tactaccccg ctcaatgcgg gtttcctacg acatctcctt caccaccata gctattctag
     8341 ctcggggatc agaattgtcg agttaccttt caactcaacc aatcatggac ttccacctgg
     8401 cattgaaaac actgataaac ttacactccc acttgtagta tcactttttc attcaaccat
     8461 ttctcttgac cctcacctta gagattatat ttcccgccat ttctcccctg cgcgccctcc
     8521 tctgtgtgtc atacatgatg tgttccttgg ttgggttgat caagttgcta aagacgtggg
     8581 ctcaactggt gttgttttta ctacgggtgg cgcgtatggt acaagcgcat atgtgtccat
     8641 ttggaatgat ctgcctcacc agaattactc tgatgatcaa gagtttccgc ttcctggttt
     8701 cccggagaat cataaattcc gacgttctca acttcatcgg tttctgaggt atgccgatgg
     8761 atcagatgat tggtcgaaat atttccaacc gcaattgagg caatcaatga agagttttgg
     8821 atggctatgt aattcagttg aggaaatcga aacacttggg tttagtatcc tcaggaacta
     8881 cactaaacta cccatttggg gtattggacc gttgatagct tcacctgtac aacattcatc
     8941 atctgataat aacagtactg gtgccgagtt tgttcaatgg ttgagcttga aagaaccaga
     9001 ttctgtatta tacatctcat ttggatcaca gaacacaatt tcaccaaccc agatgatgga
     9061 actagcagcc ggtttggagt caagtgagaa gccgtttttg tgggtgattc gagcaccatt
     9121 tgggttcgat atcaatgagg aaatgaggcc agaatggcta ccagagggat tcgaggagcg
     9181 aatgaaggtg aaaaaacaag gaaagttggt gtataagttg ggaccacagt tggagatact
     9241 taaccatgag tcaatcggag ggttcttaac tcattgtggg tggaattcga tccttgagtc
     9301 acttcgagaa ggtgtgccta tgttagggtg gccattggca gccgaacaag cttataattt
     9361 gaagtatttg gaggacgaaa tgggggttgc agtcgagtta gcgaggggat tggaaggaga
     9421 gataagtaaa gagaaagtga agagaattgt ggagatgatt ttagagagaa atgaaggaag
     9481 taaaggatgg gaaatgaaaa atagagcagt agaaatgggg aagaaactta aagacgctgt
     9541 caatgaggag aaggaactga agggttcttc tgttaaggca atagatgatt tcttagatgc
     9601 ggtcatgcaa gctaagttgg aaccttctct tcaataagct tctctagcta gagtcgatcg
     9661 acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt
     9721 cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     9781 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc
     9841 cagatcgctc agagaggatg cacatgtgac cgagggacac gaagtgatcc gtttaaacta
     9901 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     9961 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
    10021 caaccacagg gttcccctcg ggagtcagcc gtgcggctgc atgaaatcct ggccggtttg
    10081 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
    10141 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
    10201 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
    10261 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
    10321 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
    10381 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
    10441 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
    10501 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
    10561 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
    10621 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
    10681 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
    10741 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
    10801 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
    10861 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
    10921 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
    10981 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
    11041 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
    11101 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
    11161 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
    11221 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
    11281 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
    11341 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
    11401 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
    11461 agaatcccgg caaccgccgg cagccggtgc gtcgtcgatt aggaagccgc ccaagggcga
    11521 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
    11581 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
    11641 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
    11701 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
    11761 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
    11821 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
    11881 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
    11941 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
    12001 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagattac
    12061 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
    12121 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
    12181 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
    12241 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
    12301 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
    12361 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
    12421 tcgaaaaagc ttctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
    12481 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
    12541 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
    12601 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaca
    12661 gctgcaaaaa gcgcccacat caaggctccg agtgcgcgga acccctattt gtttattttt
    12721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    12781 atattgaaaa aggaagagta tggctaaaat gagaatatca ccggaattga aaaaactgat
    12841 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
    12901 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
    12961 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
    13021 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
    13081 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
    13141 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
    13201 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
    13261 ggattgcgaa aactgggaag aggacactcc atttaaagat ccgcgcgagc tgtatgattt
    13321 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
    13381 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
    13441 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg ctcagggagg atatcgggga
    13501 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
    13561 aaaatattat attttactgg atgaattgtt ttagctgtca gaccaagttt actcatatat
    13621 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    13681 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    13741 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    13801 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    13861 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    13921 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    13981 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    14041 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    14101 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    14161 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    14221 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    14281 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    14341 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctgctcg gatctgttgg
    14401 accggacagt agtcatggtt gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
    14461 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
    14521 cgcttagaca acttaataac acattgcgga cgtttttaat gtactggggt tgaacactct
    14581 gtgcc
//
