LOCUS       sequence-323057-       13984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multigene construct containing the betalain pigment biosynthetic 
            genes under the control of the NbPT5b promoter for visualisation of 
            arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13984)
  AUTHORS   Timoneda A, Yunusov T, Quan C, Gavrin A, Brockington SF, Schornack S
  TITLE     MycoRed: Betalain pigments enable in vivo real-time visualisation of
            arbuscular mycorrhizal colonisation.
  JOURNAL   PLoS Biol. 2021 Jul 14;19(7):e3001326. doi: 
            10.1371/journal.pbio.3001326.
  PUBMED    34260583
REFERENCE   2  (bases 1 to 13984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2021-07-14"; volume: "19(7):e3001326. doi"; pages: " 
            10.1371/journal.pbio.3001326"
COMMENT     Sequence Label: NbPT5b-p3
FEATURES             Location/Qualifiers
     source          1..13984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    36..60
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             369..998
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1427..2500
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2566..2760
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        2822..2926
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2927..3721
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3892..4480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4381..4400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4616..4640
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(4748..5000)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(5600..5782)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(5636..5814)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      7822..8074
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    13783..13957
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
       61 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
      121 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga gtcagccgtg
      181 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
      241 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
      301 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
      361 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
      421 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
      481 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
      541 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
      601 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
      661 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
      721 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
      781 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
      841 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
      901 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
      961 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     1021 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     1081 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     1141 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     1201 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     1261 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     1321 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     1381 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     1441 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     1501 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     1561 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgtc
     1621 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     1681 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     1741 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     1801 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     1861 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     1921 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     1981 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     2041 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     2101 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     2161 agctgattgg atgtaccgcg agattacaga aggcaagaac ccggacgtgc tgacggttca
     2221 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     2281 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     2341 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     2401 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     2461 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     2521 gcaaattgcc ctagcagggg aaaaaggtcg aaaaagcttc tttcctgtgg atagcacgta
     2581 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     2641 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     2701 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     2761 actgtctggc cagcgcacag ccgaacagct gcaaaaagcg cccacatcaa ggctccgagt
     2821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag
     2941 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
     3001 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
     3061 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
     3121 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
     3181 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
     3241 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
     3301 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
     3361 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagagg acactccatt
     3421 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
     3481 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
     3541 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
     3601 ccggtcgctc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
     3661 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
     3721 gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3781 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3841 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4021 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4501 tttacggttc ctgctcggat ctgttggacc ggacagtagt catggttgat gggctgcctg
     4561 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
     4621 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
     4681 ttttaatgta ctggggttga acactctgtg ccgaattcgg atccagcgct ccaattcagc
     4741 aagcgtcgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
     4801 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
     4861 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
     4921 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
     4981 tgccaaatgt ttgaacgatc tgcttgactc tagatccaga gtcccgctca aatctcagtc
     5041 acaggaagaa caggtctagg aggaacagga agagagaaat caagctgcca gaatcccaca
     5101 tcatgccaat ttccatgttt gaaaccagca gctctaagca ttcccctagg agcataacca
     5161 agagcctcat gcatcctcac agaaggatca ttaggaagtc caatcacagc aaccacagac
     5221 ttgaaaccct gagcctccaa agacttcaaa agatgagtgt aaagagtaga tccaagacca
     5281 gttctttgat gcctaggaga cacgtaaaca gtagactcag cagtccaatc gtaagcattc
     5341 ctagccttcc aaggaccagc ataagcaata ccagccactt caccatccac ttcagcaaca
     5401 agccaaggat acctctctct aagcctcaca agatcatcag tccactcttg tggttcttga
     5461 ggctcagtcc tgaaattcac agtagaagtc tcaatgtaat gattcacaat agtgcacaca
     5521 gcaggcatat cagcctcagt agcccttcta atatcagcag gccttctttc aggagacatg
     5581 cgaaacgatc cagatccggt gcagattatt tggattgaga gtgaatatga gactctaatt
     5641 ggataccgag gggaatttat ggaacgtcag tggagcattt tgacaagaaa tatttgctag
     5701 ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta
     5761 gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag
     5821 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
     5881 attctccgct ccgcaagaat tcaagcttgg aggtgagaaa tttaccaaat acctataaat
     5941 aatgtatcag ccgtatataa ataacgtata agagatgttt atacactatt atatattact
     6001 tatataatgt tatacattat tatacactat tatacaaatg aatcatatca atggagcttc
     6061 actgattctt cttcttcttt gggcatcttc tgaaattcaa ctcaaatcta cctcaaatct
     6121 gctccaaatt attttaaatt tgagttttga acttctcttg acgatcccaa tcaattgggc
     6181 caacacctaa tccaaacaac taacaaaatc gaaaaatcta attttaaaat tgaagcttca
     6241 aatgaccttt aatggtgact ccaaaaataa taattctctt acgtggtatt ttttcttctc
     6301 atcttaatag tgattatcaa ttttgaatat ggaaggagaa ttaaaaaata tatataatgg
     6361 tagggaaact ttggggtgca aagtcgtgaa agaagaggaa gaagaagaac gaagaaaaga
     6421 aaaaaatagg gtgggctaac tggtgaaatt tttttccaaa ttatatataa cctaggctat
     6481 atcatgtaag aacttcaaat gtgggccatt ttttgatgga ttgttggacc aaatgacaag
     6541 gagcgtaatt cccccttcat tttatatggc acactaggat attatgagtt taataaataa
     6601 agaatttttt ttatgatttt aaacatgtcg tgactataaa attgtgttat taaagtaaac
     6661 taagaagttt gaagttagat tatatcaatt atatagattt gttttttttt tgacaccaac
     6721 taacaagaaa aagttgtcat ataaagcata agcatgcgcg ctttttgtcc taggttattt
     6781 ttgattgaag catatgcttg atttaagcaa agaagaagga tatcctgtgg atacttatcc
     6841 tcaatttctt gttgtagcca ctgagcgctt aggattaaac gtcatttaat tcacattgtg
     6901 cgctgagtct atatatggga gttacataca ccaaaacagt accatgaaaa cacaaccaaa
     6961 atttgcagta aaaagcaaac aatgaaaatg atgaatggag aagatgctaa tgatcaaatg
     7021 atcaaagaaa gcttcttcat aacacatgga aacccaatat taacagtaga agatacacat
     7081 ccattaagac ctttctttga aacttggaga gagaaaatct tttctaagaa acctaaggca
     7141 attcttatta tttctggtca ttgggaaact gttaaaccta ctgttaatgc tgtccatatc
     7201 aatgatacta tccatgattt tgatgactat cctgctgcta tgtaccagtt caagtatcca
     7261 gctcctgggg caccagaatt ggcaagaaaa gtagaggaaa ttctgaaaaa atcgggtttc
     7321 gaaacggcgg aaactgacga aaaacgtggg cttgatcatg gtgcatgggt acctctaatg
     7381 ctaatgtatc ctgaggctga tataccagta tgtcagctct cagttcagcc gcatttagat
     7441 ggaacatacc attacaactt aggacgagca ttagctccct tgaaaaacga tggcgtattg
     7501 atcattggtt caggaagtgc aactcaccct ttggatgaaa ctcctcatta ttttgatgga
     7561 gttgcacctt gggcagctgc ctttgattca tggcttcgta aagctctcat aaatggaagg
     7621 tttgaagaag tgaatatata tgaaacgaaa gcaccaaatt ggaaattagc acatcctttc
     7681 ccagaacatt tttatccatt gcatgttgtt cttggcgctg ctggtgaaaa atggaaggct
     7741 gagcttattc atagcagttg ggatcatggc accttgtgtc acggctccta caagttcact
     7801 tcagcctagg cttgtcaagc agatcgttca aacatttggc aataaagttt cttaagattg
     7861 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
     7921 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     7981 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     8041 ttatcgcgcg cggtgtcatc tatgttacta gatcgacgct actagaattc gagctcggag
     8101 gtgagaaatt taccaaatac ctataaataa tgtatcagcc gtatataaat aacgtataag
     8161 agatgtttat acactattat atattactta tataatgtta tacattatta tacactatta
     8221 tacaaatgaa tcatatcaat ggagcttcac tgattcttct tcttctttgg gcatcttctg
     8281 aaattcaact caaatctacc tcaaatctgc tccaaattat tttaaatttg agttttgaac
     8341 ttctcttgac gatcccaatc aattgggcca acacctaatc caaacaacta acaaaatcga
     8401 aaaatctaat tttaaaattg aagcttcaaa tgacctttaa tggtgactcc aaaaataata
     8461 attctcttac gtggtatttt ttcttctcat cttaatagtg attatcaatt ttgaatatgg
     8521 aaggagaatt aaaaaatata tataatggta gggaaacttt ggggtgcaaa gtcgtgaaag
     8581 aagaggaaga agaagaacga agaaaagaaa aaaatagggt gggctaactg gtgaaatttt
     8641 tttccaaatt atatataacc taggctatat catgtaagaa cttcaaatgt gggccatttt
     8701 ttgatggatt gttggaccaa atgacaagga gcgtaattcc cccttcattt tatatggcac
     8761 actaggatat tatgagttta ataaataaag aatttttttt atgattttaa acatgtcgtg
     8821 actataaaat tgtgttatta aagtaaacta agaagtttga agttagatta tatcaattat
     8881 atagatttgt tttttttttg acaccaacta acaagaaaaa gttgtcatat aaagcataag
     8941 catgcgcgct ttttgtccta ggttattttt gattgaagca tatgcttgat ttaagcaaag
     9001 aagaaggata tcctgtggat acttatcctc aatttcttgt tgtagccact gagcgcttag
     9061 gattaaacgt catttaattc acattgtgcg ctgagtctat atatgggagt tacatacacc
     9121 aaaacagtac catgaaaaca caaccaaaat ttgcagtaaa aagcaaacaa tggatcatgc
     9181 aacattagca atgatactag ccatttggtt catttctttt catttcataa aattactttt
     9241 tagccaacaa actaccaaac ttcttcctcc tggtccaaaa ccattgccaa taattggcaa
     9301 catcttagaa gttggtaaaa aacctcatcg ttcatttgct aatcttgcta aaattcacgg
     9361 ccctttaata tcgttacgtc taggaagtgt aacaactatt gttgtatcat cagcagatgt
     9421 agctaaagaa atgttcttaa aaaaagacca ccctctttct aaccgtacta ttcctaattc
     9481 tgtcacggcc ggtgaccacc ataaacttac catgtcgtgg ttgccggttt cgccgaaatg
     9541 gcggaatttt cgtaagatta cagccgtcca tttgctttct cctcaaagac ttgatgcttg
     9601 ccaaaccttt cgccatgcga aggtgcaaca gctttatgaa tatgtgcaag aatgtgcaca
     9661 aaaaggccaa gctgttgata ttggcaaagc tgcatttact acctccctta atttgttatc
     9721 taaactattt ttttcagtgg aattagccca ccataaatca caaacttctc aagagtttaa
     9781 ggaactaata tggaacatta tggaagatat tgggaaacct aactatgctg attattttcc
     9841 tattttaggc tgtgttgatc catcaggtat tcgacgaaga ttagcatgta gttttgacaa
     9901 gttgattgca gtttttcaga gtataatatg tgaaaggctt gcgcctgatt cttcaactgc
     9961 aacaacaacg acgactgatg atgtgctaga cgttcttctt cagctcttca aacaaaatga
    10021 gctcactatg ggcgagatta accatttgct cgtcgacatt tttgatgctg gaacagacac
    10081 tacatcaagt acttttgagt gggtcatggc agagttaatt aggaatcctg aaatgatgga
    10141 aaaggctcaa gaagaaatta agcaagtatt aggcaaagat aaacaaattc aggaatcaga
    10201 cattattaac ctaccttact tacaagccat tatcaaagaa actttgcgac tacatccacc
    10261 aactgtattt cttttgcctc gtaaagccga cactgatgtt gaactatatg gttatattgt
    10321 gcctaaagat gcacaaatac ttgttaactt atgggctatt ggaagagatc ctaatgcctg
    10381 gcaaaatgct gatatttttt cgcccgaaag atttataggg tgtgaaattg atgtcaaagg
    10441 aagagatttt ggactcttac cttttggagc tggaagaagg atatgtcctg ggatgaattt
    10501 ggccattaga atgttaactt tgatgctagc tactttgctt caattcttca attggaagct
    10561 tgaaggagac ataagtccaa aagacttaga catggatgag aaatttggga ttgcgttaca
    10621 aaagacaaag cctttaaaac ttattccaat acctaggtat tgagcttgct ctcaagatca
    10681 aaggcttaaa aagctggggt tttatgaatg ggatcaaagt ttcttttttt cttttatatt
    10741 tgcttctcca tttgtttgtt tcatttccct ttttgttttc gtttctatga tgcacttgtg
    10801 tgtgacaaac tctctgggtt tttacttacg tctgcgtttc aaaaaaaaaa accgctttcg
    10861 ttttgcgttt tagtcccatt gttttgtagc tctgagtgat cgaattgatg cctctttatt
    10921 ccttttgttc cctataattt ctttcaaaac tcagaagaaa aaccttgaaa ctctttgcaa
    10981 tgttaatata agtattgtat aagattttta ttgatttggt tattagtctt acttttgcta
    11041 cctccatctt cacttggaac tgatattctg aatagttaaa gcgttacatg tgttccattc
    11101 acaaatgaac ttaaactagc acaaagtcag atattttaag atcgcaccat ttcgctttac
    11161 gaattcccat ggggaggtga gaaatttacc aaatacctat aaataatgta tcagccgtat
    11221 ataaataacg tataagagat gtttatacac tattatatat tacttatata atgttataca
    11281 ttattataca ctattataca aatgaatcat atcaatggag cttcactgat tcttcttctt
    11341 ctttgggcat cttctgaaat tcaactcaaa tctacctcaa atctgctcca aattatttta
    11401 aatttgagtt ttgaacttct cttgacgatc ccaatcaatt gggccaacac ctaatccaaa
    11461 caactaacaa aatcgaaaaa tctaatttta aaattgaagc ttcaaatgac ctttaatggt
    11521 gactccaaaa ataataattc tcttacgtgg tattttttct tctcatctta atagtgatta
    11581 tcaattttga atatggaagg agaattaaaa aatatatata atggtaggga aactttgggg
    11641 tgcaaagtcg tgaaagaaga ggaagaagaa gaacgaagaa aagaaaaaaa tagggtgggc
    11701 taactggtga aatttttttc caaattatat ataacctagg ctatatcatg taagaacttc
    11761 aaatgtgggc cattttttga tggattgttg gaccaaatga caaggagcgt aattccccct
    11821 tcattttata tggcacacta ggatattatg agtttaataa ataaagaatt ttttttatga
    11881 ttttaaacat gtcgtgacta taaaattgtg ttattaaagt aaactaagaa gtttgaagtt
    11941 agattatatc aattatatag atttgttttt tttttgacac caactaacaa gaaaaagttg
    12001 tcatataaag cataagcatg cgcgcttttt gtcctaggtt atttttgatt gaagcatatg
    12061 cttgatttaa gcaaagaaga aggatatcct gtggatactt atcctcaatt tcttgttgta
    12121 gccactgagc gcttaggatt aaacgtcatt taattcacat tgtgcgctga gtctatatat
    12181 gggagttaca tacaccaaaa cagtaccatg aaaacacaac caaaatttgc agtaaaaagc
    12241 aaacaatgac cgccattaaa atgaacacca atggtgaagg tgaaacacaa catatactaa
    12301 tgataccttt catggcgcaa gggcatttac gtcctttcct tgagcttgct atgtttctat
    12361 ataaacgaag tcattttatc attactcttc ttactacccc gctcaatgcg ggtttcctac
    12421 gacatctcct tcaccaccat agctattcta gctcggggat cagaattgtc gagttacctt
    12481 tcaactcaac caatcatgga cttccacctg gcattgaaaa cactgataaa cttacactcc
    12541 cacttgtagt atcacttttt cattcaacca tttctcttga ccctcacctt agagattata
    12601 tttcccgcca tttctcccct gcgcgccctc ctctgtgtgt catacatgat gtgttccttg
    12661 gttgggttga tcaagttgct aaagacgtgg gctcaactgg tgttgttttt actacgggtg
    12721 gcgcgtatgg tacaagcgca tatgtgtcca tttggaatga tctgcctcac cagaattact
    12781 ctgatgatca agagtttccg cttcctggtt tcccggagaa tcataaattc cgacgttctc
    12841 aacttcatcg gtttctgagg tatgccgatg gatcagatga ttggtcgaaa tatttccaac
    12901 cgcaattgag gcaatcaatg aagagttttg gatggctatg taattcagtt gaggaaatcg
    12961 aaacacttgg gtttagtatc ctcaggaact acactaaact acccatttgg ggtattggac
    13021 cgttgatagc ttcacctgta caacattcat catctgataa taacagtact ggtgccgagt
    13081 ttgttcaatg gttgagcttg aaagaaccag attctgtatt atacatctca tttggatcac
    13141 agaacacaat ttcaccaacc cagatgatgg aactagcagc cggtttggag tcaagtgaga
    13201 agccgttttt gtgggtgatt cgagcaccat ttgggttcga tatcaatgag gaaatgaggc
    13261 cagaatggct accagaggga ttcgaggagc gaatgaaggt gaaaaaacaa ggaaagttgg
    13321 tgtataagtt gggaccacag ttggagatac ttaaccatga gtcaatcgga gggttcttaa
    13381 ctcattgtgg gtggaattcg atccttgagt cacttcgaga aggtgtgcct atgttagggt
    13441 ggccattggc agccgaacaa gcttataatt tgaagtattt ggaggacgaa atgggggttg
    13501 cagtcgagtt agcgagggga ttggaaggag agataagtaa agagaaagtg aagagaattg
    13561 tggagatgat tttagagaga aatgaaggaa gtaaaggatg ggaaatgaaa aatagagcag
    13621 tagaaatggg gaagaaactt aaagacgctg tcaatgagga gaaggaactg aagggttctt
    13681 ctgttaaggc aatagatgat ttcttagatg cggtcatgca agctaagttg gaaccttctc
    13741 ttcaataagc ttctctagct agagtcgatc gacaagctcg agtttctcca taataatgtg
    13801 tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca
    13861 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
    13921 attcctaaaa ccaaaatcca gtactaaaat ccagatcgct cagagaggat gcacatgtga
    13981 ccga
//
