LOCUS       sequence-32306-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     Sekelsky lab Drosophila phiC31 cloning plasmids
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDEST-attB
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..690
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(97..116)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
       61 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      121 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
      181 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
      241 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
      301 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
      361 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
      421 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
      481 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
      541 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
      601 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
      661 tactgcgatg agtggcaggg cggggcgtaa agatctggat ccggcttact aaaagccaga
      721 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat
      781 acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca
      841 gcgacagcta tcagttgctc aagg
//
