LOCUS       sequence-32309-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Soellu C, Pars K, Cornu TI, Thibodeau-Beganny S, Maeder ML, Joung 
            JK, Heilbronn R, Cathomen T
  TITLE     Autonomous zinc-finger nuclease pairs for targeted chromosomal 
            deletion.
  JOURNAL   Nucleic Acids Res. 2010 Dec 1. 38(22):8269-76.
  PUBMED    20716517
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2010 Dec 1. 38(22):8269-76."
COMMENT     Sequence Label: pMG10
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..123
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             130..150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             791..844
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             851..916
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc caccatggat
       61 tataaggatc acgatggcga ctacaaggac cacgatattg actacaaaga cgatgacgat
      121 aaaatggctc ctaagaaaaa gcgcaaagtc ggtatccatg gcgttcccgc tagctaacgc
      181 aagatctcag ctggtgaagt ccgagctcga agaaaaaaag agcgagctgc gccacaagct
      241 caagtacgtg ccccacgagt acatcgagct gatcgagatc gcccgcaaca gcacccaaga
      301 ccgcatcctg gagatgaaag tgatggagtt cttcatgaag gtgtacggct accgcggcaa
      361 gcacctgggc ggctcccgca agcccgatgg cgccatctac accgtgggct cccccatcga
      421 ctatggcgtc attgtcgaca ccaaggccta ctccggcggc tacaacttac ccatcggtca
      481 ggccgacgag atgcaacgct acgtgaagga gaaccagacc cgcaataagc acattaatcc
      541 caacgagtgg tggaaggtgt acccctcctc cgtgaccgag ttcaaattcc tgttcgtgtc
      601 cggccacttc aagggcaatt ataaggccca actgacccgc ctgaaccaca agaccaactg
      661 caacggcgcc gtgctgtccg tggaggaact gctgatcggc ggcgagatga tcaaggctgg
      721 taccctgacc ctggaagagg tgcgccgcaa gttcaacaat ggtgaaatca atttcaggtc
      781 cggcggcgga gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
      841 ccctaggatg gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa
      901 ggatgacgat gacaagatgg cccccaagaa gaagagga
//
