LOCUS       sequence-32312-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pLKO.1 RagA shRNA #2
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..147
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    302..419
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
       61 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      121 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      181 tatcttgtgg aaaggacgaa acaccggccg gtttgagaag atcagcaact cgagttgctg
      241 atcttctcaa accggttttt gaattctcga cctcgagaca aatggcagta ttcatccaca
      301 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
      361 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
      421 gggtttatta cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg
      481 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
      541 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
      601 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
      661 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaaacgg aagcccgcac
      721 gtctcactag taccctcgca gacg
//
