LOCUS       sequence-32313-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pLKO.1 RagA shRNA #1
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..137
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    292..409
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
       61 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      121 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      181 aaaggacgaa acaccgggtc gataatcttc gccaattact cgagtaattg gcgaagatta
      241 tcgacttttt gaattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag
      301 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      361 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
      421 cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg cgccttttcc
      481 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca
      541 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct
      601 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag
      661 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta
      721 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg
      781 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg
      841 aggcg
//
