LOCUS       sequence-32315-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Wall NR, Wickersham IR, Cetin A, De La Parra M, Callaway EM
  TITLE     Monosynaptic circuit tracing in vivo through Cre-dependent targeting
            and complementation of modified rabies virus.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21848-53. doi: 
            10.1073/pnas.1011756107
  PUBMED    21115815
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2010-12-14- 14"; 
            volume: "107"; issue: "50"; pages: "21848-53"
COMMENT     Sequence Label: pAAV-EF1a-FLEX-GT
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(564..597)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(660..693)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgtacagaa gcttggtagt ggagagggca gaggaagtct gctaacatgc nnngacgtcg
       61 aggagaatcc tggcccaatc gatgcgcggc tgctgcccgc gctgctgctg ctgctgctgc
      121 ccggtaacgt gaccggtaac gggtccggta acggttcttt gtcccgttgc ccccccggtc
      181 agttccgctg ctcggagccg cccggtgccc acggggagtg ttacccgcag gactggctgt
      241 gcgacggaca ccccgactgc gacgacgggc gggacgagtg gggctgcggg accagcgcga
      301 tccccgcggt gcccacggac aacggcacag aggctcccac tgcccctgct cctggacgtg
      361 ctctgccagc caggaatcac ggccgcatgt ggatgctgat cactgcagtg ctcctgtgct
      421 gcctggtagc tgtgggtggt atcgctgcat gggggaagtc caaagcaaaa agcaggtctg
      481 acatcttcag tcttgaaagc gcatccaagg agctgctggt gcctgacaag agccaggcag
      541 acttgttctc cgctagttga agtataactt cgtatagcat acattatacg aagttatggg
      601 tcgatggtga tgcttggcaa ttcgggtcga tggtgaagca ttggtcttcc tattttgata
      661 taacttcgta taggatactt tatacgaagt tatggccatg gtggcggctc tagaggatcc
      721 ggtacctcac gacacctgaa atggaagaaa aaaactttga accactgtct gaggctt
//
