LOCUS       sequence-32316-e         501 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 501)
  TITLE     New vectors Aug. 2010
REFERENCE   2  (bases 1 to 501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR4-GLU-GLU (w719-1)
FEATURES             Location/Qualifiers
     source          1..501
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..187
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(254..271)
                     /codon_start=1
                     /product="epitope tag from middle T antigen of mouse 
                     polyoma virus"
                     /label=Glu-Glu tag
                     /translation="EYMPME"
     protein_bind    complement(283..382)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(401..424)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(432..459)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
       61 acacgggcca gagctgcagc tggatggcaa ataatgattt tattttgact gatagtgacc
      121 tgttcgttgc aacaaattga taagcaatgc tttcttataa tgccaacttt gtacaagaaa
      181 gctgggtcta gatatctcga gtgcggccgc gaattcggta ccggatccag tcgactgaat
      241 tggttcccat gccctccata ggcatgtact cttccatggt ggagcctgct tttttgtaca
      301 aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca
      361 gtcaaaataa aatcattatt tggggcccga gcttaagtaa ctaactaaca ggaagagttt
      421 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
      481 tttatggcgg gcgtcctgcc c
//
