LOCUS       sequence-323164-        3321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nicotiana benthamiana PT5b promoter sequence (1068 bp immediately 
            upstream of start codon) in pICH41295 MoClo Golden Gate level 0 
            acceptor for Pro+5U modules..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Timoneda A, Yunusov T, Quan C, Gavrin A, Brockington SF, Schornack S
  TITLE     MycoRed: Betalain pigments enable in vivo real-time visualisation of
            arbuscular mycorrhizal colonisation.
  JOURNAL   PLoS Biol. 2021 Jul 14;19(7):e3001326. doi: 
            10.1371/journal.pbio.3001326.
  PUBMED    34260583
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2021-07-14"; volume: "19(7):e3001326. doi"; pages: " 
            10.1371/journal.pbio.3001326"
COMMENT     Sequence Label: pL0-NbPT5bPro
FEATURES             Location/Qualifiers
     source          1..3321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..58)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        126..230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             452..1243
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1414..2002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1903..1922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2156..2173
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt
       61 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac
      241 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      301 tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca
      361 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
      421 gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc
      481 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
      541 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt
      601 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
      661 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
      721 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
      781 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
      841 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
      901 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
      961 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1021 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1081 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1141 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1201 ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta
     1261 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1321 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1561 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2041 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2101 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2161 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     2221 gccgattcat taatcactct gtggtctcag gaggtgagaa atttaccaaa tacctataaa
     2281 taatgtatca gccgtatata aataacgtat aagagatgtt tatacactat tatatattac
     2341 ttatataatg ttatacatta ttatacacta ttatacaaat gaatcatatc aatggagctt
     2401 cactgattct tcttcttctt tgggcatctt ctgaaattca actcaaatct acctcaaatc
     2461 tgctccaaat tattttaaat ttgagttttg aacttctctt gacgatccca atcaattggg
     2521 ccaacaccta atccaaacaa ctaacaaaat cgaaaaatct aattttaaaa ttgaagcttc
     2581 aaatgacctt taatggtgac tccaaaaata ataattctct tacgtggtat tttttcttct
     2641 catcttaata gtgattatca attttgaata tggaaggaga attaaaaaat atatataatg
     2701 gtagggaaac tttggggtgc aaagtcgtga aagaagagga agaagaagaa cgaagaaaag
     2761 aaaaaaatag ggtgggctaa ctggtgaaat ttttttccaa attatatata acctaggcta
     2821 tatcatgtaa gaacttcaaa tgtgggccat tttttgatgg attgttggac caaatgacaa
     2881 ggagcgtaat tcccccttca ttttatatgg cacactagga tattatgagt ttaataaata
     2941 aagaattttt tttatgattt taaacatgtc gtgactataa aattgtgtta ttaaagtaaa
     3001 ctaagaagtt tgaagttaga ttatatcaat tatatagatt tgtttttttt ttgacaccaa
     3061 ctaacaagaa aaagttgtca tataaagcat aagcatgcgc gctttttgtc ctaggttatt
     3121 tttgattgaa gcatatgctt gatttaagca aagaagaagg atatcctgtg gatacttatc
     3181 ctcaatttct tgttgtagcc actgagcgct taggattaaa cgtcatttaa ttcacattgt
     3241 gcgctgagtc tatatatggg agttacatac accaaaacag taccatgaaa acacaaccaa
     3301 aatttgcagt aaaaagcaaa c
//
