LOCUS       sequence-32317-e         305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 305)
  TITLE     New vectors Aug. 2010
REFERENCE   2  (bases 1 to 305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR4-GLU-GLU (w719-1)
FEATURES             Location/Qualifiers
     source          1..305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             108..125
                     /codon_start=1
                     /product="epitope tag from middle T antigen of mouse 
                     polyoma virus"
                     /label=Glu-Glu tag
                     /translation="EYMPME"
     protein_bind    complement(192..291)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 tttttataat gccaactttg tacaaaaaag caggctccac catggaagag tacatgccta
      121 tggagggcat gggaaccaat tcagtcgact ggatccggta ccgaattcgc ggccgcactc
      181 gagatatcta gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
      241 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccggc
      301 tgcaa
//
