LOCUS       sequence-323174-       10854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10854)
  TITLE     Immunoprecipitation of the endoplasmic reticulum (ER-IP)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: control (pGS701)
FEATURES             Location/Qualifiers
     source          1..10854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3350..4535
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3581..4526
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4483..4503
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4625..4705
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    5472..6024
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5616..5633)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5843..5862
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6033..6428
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6438..6494
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6504..7049
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             7059..7079
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7086..7674
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7139..7159)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7557..7568)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8060..8293
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8365..8486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8402..8421)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8456..8475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8588..8607
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8872..8890)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8958..9062
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9063..9131,9132..9923)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9281..9300)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10094..10682
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10583..10602
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgata ccgtcgagtt tactccctat
     2281 tctagacaaa tgcttgtcct tccttcctgt aacacagcca ttaaaccagg agcatcgccc
     2341 ttccccggcc ctcaggtaag aggaccaaat accgtagccg tttccaattt cagtccttta
     2401 gcgccacctg gtgctaacta ctctatcacg cttttatcca ataactacct ttgtaaattt
     2461 cctttcaaaa gttctggccg ggcgcggtgg ctcacgcttg taatcccagc actttgtgag
     2521 gggtcaggag ttcgagacat taatagggag gtggtccctg cagttacgcc aatgataacc
     2581 cccgccagaa aaatcttagt agccttccct ttttgttttc cgtgccccaa ctcggcggat
     2641 tgactcggcc ccttccggaa acacccgaat caacttctag tcaaattatt gttcacgccg
     2701 caatgaccca cccctggccc gcgtctgtgg aactgacccc tggtgtacag gagagttcgc
     2761 tgctgaaagt ggtcccaaag gggtactagt ttttaagctc ccaactcccc ctcccccagc
     2821 gtctggagga ttccacaccc tcgcaccgca ggggcgagga agtgggcgga gtccggtttt
     2881 ggcgccagcc gctgaggctg ccaagcagaa aagccaccgc tgaggagact ccggtcactg
     2941 tcctcgcccc gcctccccct tccctcccct tggggaccac cgggcgccac gccgcgaacg
     3001 gtaagtgccg cggtcgtcgg cgcctccgcc ctccccctag ggccccaatt cccagcgggc
     3061 gcggcgcgcg gcccctcccc ccgccgggcg cgcgcccgct gccccgccct tcgtggccgc
     3121 ccggcgtggg cggtgccacc cctccccccg gcggccccgc gcgcagctcc cggctccctc
     3181 ccccttcgga tgtggcttga gctgtaggcg cggagtttaa ttaacgcgta ccgtcgacct
     3241 cgacggatcg ggagatctcg accgagcttt gcaaagatgg ataaagtttt aaacagagag
     3301 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc
     3361 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     3421 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     3481 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     3541 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     3601 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
     3661 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     3721 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     3781 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     3841 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     3901 tgtaaatgcg ggccaagatg atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     3961 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     4021 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     4081 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     4141 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     4201 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     4261 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     4321 gatcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     4381 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     4441 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     4501 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat tagcttgacc aagcttggta
     4561 ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattatcaca aggccgccac
     4621 catgtaccca tacgatgttc cagattacgc ttatccctac gacgtgcctg attatgcata
     4681 cccatatgat gtccccgact atgccggcgg atcaagtaag ggggaggagc tgtttacggg
     4741 tgtagtaccc atacttgtcg aactggacgg tgatgttaat ggacataagt ttagtgtcag
     4801 aggggaaggg gaaggggatg caacgaatgg aaagctgacg ctcaagttca tctgtacaac
     4861 tggcaaactc ccggtgcctt ggccaactct cgtaaccaca ttgacatacg gagtccagtg
     4921 ctttagtagg tatccggatc atatgaagca gcatgatttc tttaagtcag ccatgccaga
     4981 gggttatgtc caggaacgaa ctatcagttt taaggatgat ggaacgtaca aaactcgagc
     5041 agaagtcaag ttcgaaggcg atactttggt aaaccgcata gaactcaaag ggatcgactt
     5101 caaagaagat ggaaacatac tgggccataa actggaatat aactttaact cccacaatgt
     5161 ttacatcact gccgataagc agaagaacgg tattaaggcc aattttaaga ttaggcataa
     5221 tgtagaagat gggtcagttc aattggcgga tcattatcag caaaacacgc cgataggaga
     5281 cggtcccgtg cttctcccag acaaccacta tcttagcaca cagtctaagc tgagtaagga
     5341 ccctaatgag aagagggatc acatggtact tctggaattt gttacggcgg caggtatcac
     5401 ccacggtatg gatgaactct acaagggtgg tggttctgga ggcggcggat cacgcgttgc
     5461 agtttaaact aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     5521 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     5581 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     5641 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     5701 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     5761 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     5821 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     5881 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     5941 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     6001 cctttgaaaa acacgatgat aatatggcca caatggccaa gcctttgtct caagaagaat
     6061 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     6121 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     6181 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     6241 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     6301 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     6361 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     6421 gggagggcgg atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag
     6481 agaatcctgg accgggatcc ggcatggaca aagactgcga aatgaagcgc accaccctgg
     6541 atagccctct gggcaagctg gaactgtctg ggtgcgaaca gggcctgcac cgtatcatct
     6601 tcctgggcaa aggaacatct gccgccgacg ccgtggaagt gcctgcccca gccgccgtgc
     6661 tgggcggacc agagccactg atgcaggcca ccgcctggct caacgcctac tttcaccagc
     6721 ctgaggccat cgaggagttc cctgtgccag ccctgcacca cccagtgttc cagcaggaga
     6781 gctttacccg ccaggtgctg tggaaactgc tgaaagtggt gaagttcgga gaggtcatca
     6841 gctacagcca cctggccgcc ctggccggca atcccgccgc caccgccgcc gtgaaaaccg
     6901 ccctgagcgg aaatcccgtg cccattctga tcccctgcca ccgggtggtg cagggcgacc
     6961 tggacgtggg gggctacgag ggcgggctcg ccgtgaaaga gtggctgctg gcccacgagg
     7021 gccacagact gggcaagcct gggctgggtg gcgggagccc aaagaagaag cggaaggtgt
     7081 agcggaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7141 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7201 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7261 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7321 ccactggttg gggcattgcc accacctgtc agctcctttc cggaactttc gctttccccc
     7381 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7441 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7501 tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7561 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7621 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     7681 accgagcgct gctcgaggta cgccaacaga gcgagattcc gtctcaaaga aaaaaaaagt
     7741 aatgaaatga ataaaatgag tcctagagcc agtaaatgtc gtaaatgtct cagctagtca
     7801 ggtagtaaaa ggtctcaact aggcagtggc agagcaggat tcaaattcag ggctgttgtg
     7861 atgcctccgc agactctgag cgccacctgg tggtaatttg tctgtgcctc ttctgacgtg
     7921 gaagaacagc aactaacaca ctaacacggc atttactatg ggccagccat tgttctaatg
     7981 ttaactgtgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8041 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     8101 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8161 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8221 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8281 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8341 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     8401 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8461 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8521 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8581 ttttttttat ttatgcagag gccgaggccg gatcccttga gtggctttca tcctggagca
     8641 gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag
     8701 ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc
     8761 tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc
     8821 attagcaata gtgtttataa ggcccccttg ttaattcttg aagacgaaag ggcctcgtga
     8881 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     8941 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9001 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9061 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9121 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9181 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9241 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9301 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9361 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9421 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9481 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9541 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9601 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9661 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     9721 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     9781 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     9841 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     9901 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     9961 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10021 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10081 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10141 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10201 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    10261 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10321 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10381 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10441 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
    10501 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10561 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10621 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10681 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct ttttgaagct
    10741 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
    10801 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcg
//
