LOCUS       sequence-323175-       10919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10919)
  TITLE     Immunoprecipitation of the endoplasmic reticulum (ER-IP)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: ER-J-TAG (pGS730)
FEATURES             Location/Qualifiers
     source          1..10919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3350..4535
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3581..4526
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4483..4503
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4625..4705
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    5537..6089
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5681..5698)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5908..5927
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6098..6493
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6503..6559
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6569..7114
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             7124..7144
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7151..7739
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7204..7224)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7622..7633)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8125..8358
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8430..8551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8467..8486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8521..8540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8653..8672
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8937..8955)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9023..9127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9128..9196,9197..9988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9346..9365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10159..10747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10648..10667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgata ccgtcgagtt tactccctat
     2281 tctagacaaa tgcttgtcct tccttcctgt aacacagcca ttaaaccagg agcatcgccc
     2341 ttccccggcc ctcaggtaag aggaccaaat accgtagccg tttccaattt cagtccttta
     2401 gcgccacctg gtgctaacta ctctatcacg cttttatcca ataactacct ttgtaaattt
     2461 cctttcaaaa gttctggccg ggcgcggtgg ctcacgcttg taatcccagc actttgtgag
     2521 gggtcaggag ttcgagacat taatagggag gtggtccctg cagttacgcc aatgataacc
     2581 cccgccagaa aaatcttagt agccttccct ttttgttttc cgtgccccaa ctcggcggat
     2641 tgactcggcc ccttccggaa acacccgaat caacttctag tcaaattatt gttcacgccg
     2701 caatgaccca cccctggccc gcgtctgtgg aactgacccc tggtgtacag gagagttcgc
     2761 tgctgaaagt ggtcccaaag gggtactagt ttttaagctc ccaactcccc ctcccccagc
     2821 gtctggagga ttccacaccc tcgcaccgca ggggcgagga agtgggcgga gtccggtttt
     2881 ggcgccagcc gctgaggctg ccaagcagaa aagccaccgc tgaggagact ccggtcactg
     2941 tcctcgcccc gcctccccct tccctcccct tggggaccac cgggcgccac gccgcgaacg
     3001 gtaagtgccg cggtcgtcgg cgcctccgcc ctccccctag ggccccaatt cccagcgggc
     3061 gcggcgcgcg gcccctcccc ccgccgggcg cgcgcccgct gccccgccct tcgtggccgc
     3121 ccggcgtggg cggtgccacc cctccccccg gcggccccgc gcgcagctcc cggctccctc
     3181 ccccttcgga tgtggcttga gctgtaggcg cggagtttaa ttaacgcgta ccgtcgacct
     3241 cgacggatcg ggagatctcg accgagcttt gcaaagatgg ataaagtttt aaacagagag
     3301 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc
     3361 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     3421 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     3481 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     3541 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     3601 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
     3661 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     3721 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     3781 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     3841 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     3901 tgtaaatgcg ggccaagatg atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     3961 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     4021 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     4081 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     4141 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     4201 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     4261 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     4321 gatcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     4381 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     4441 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     4501 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat tagcttgacc aagcttggta
     4561 ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattatcaca aggccgccac
     4621 catgtaccca tacgatgttc cagattacgc ttatccctac gacgtgcctg attatgcata
     4681 cccatatgat gtccccgact atgccggcgg atcaagtaag ggggaggagc tgtttacggg
     4741 tgtagtaccc atacttgtcg aactggacgg tgatgttaat ggacataagt ttagtgtcag
     4801 aggggaaggg gaaggggatg caacgaatgg aaagctgacg ctcaagttca tctgtacaac
     4861 tggcaaactc ccggtgcctt ggccaactct cgtaaccaca ttgacatacg gagtccagtg
     4921 ctttagtagg tatccggatc atatgaagca gcatgatttc tttaagtcag ccatgccaga
     4981 gggttatgtc caggaacgaa ctatcagttt taaggatgat ggaacgtaca aaactcgagc
     5041 agaagtcaag ttcgaaggcg atactttggt aaaccgcata gaactcaaag ggatcgactt
     5101 caaagaagat ggaaacatac tgggccataa actggaatat aactttaact cccacaatgt
     5161 ttacatcact gccgataagc agaagaacgg tattaaggcc aattttaaga ttaggcataa
     5221 tgtagaagat gggtcagttc aattggcgga tcattatcag caaaacacgc cgataggaga
     5281 cggtcccgtg cttctcccag acaaccacta tcttagcaca cagtctaagc tgagtaagga
     5341 ccctaatgag aagagggatc acatggtact tctggaattt gttacggcgg caggtatcac
     5401 ccacggtatg gatgaactct acaagggtgg tggttctgga ggcggcggat cacctaattc
     5461 aatcatgatt gtccttgtca tgctgttgaa tatcgggttg gccattcttt ttgttcactt
     5521 tctaacttga aaactaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     5581 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     5641 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     5701 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     5761 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     5821 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     5881 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     5941 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     6001 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     6061 gttttccttt gaaaaacacg atgataatat ggccacaatg gccaagcctt tgtctcaaga
     6121 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     6181 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     6241 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     6301 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     6361 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     6421 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     6481 tgtgtgggag ggcggatccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt
     6541 cgaagagaat cctggaccgg gatccggcat ggacaaagac tgcgaaatga agcgcaccac
     6601 cctggatagc cctctgggca agctggaact gtctgggtgc gaacagggcc tgcaccgtat
     6661 catcttcctg ggcaaaggaa catctgccgc cgacgccgtg gaagtgcctg ccccagccgc
     6721 cgtgctgggc ggaccagagc cactgatgca ggccaccgcc tggctcaacg cctactttca
     6781 ccagcctgag gccatcgagg agttccctgt gccagccctg caccacccag tgttccagca
     6841 ggagagcttt acccgccagg tgctgtggaa actgctgaaa gtggtgaagt tcggagaggt
     6901 catcagctac agccacctgg ccgccctggc cggcaatccc gccgccaccg ccgccgtgaa
     6961 aaccgccctg agcggaaatc ccgtgcccat tctgatcccc tgccaccggg tggtgcaggg
     7021 cgacctggac gtggggggct acgagggcgg gctcgccgtg aaagagtggc tgctggccca
     7081 cgagggccac agactgggca agcctgggct gggtggcggg agcccaaaga agaagcggaa
     7141 ggtgtagcgg aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7201 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7261 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7321 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7381 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggaa ctttcgcttt
     7441 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7501 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7561 ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7621 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7681 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7741 tcgataccga gcgctgctcg aggtacgcca acagagcgag attccgtctc aaagaaaaaa
     7801 aaagtaatga aatgaataaa atgagtccta gagccagtaa atgtcgtaaa tgtctcagct
     7861 agtcaggtag taaaaggtct caactaggca gtggcagagc aggattcaaa ttcagggctg
     7921 ttgtgatgcc tccgcagact ctgagcgcca cctggtggta atttgtctgt gcctcttctg
     7981 acgtggaaga acagcaacta acacactaac acggcattta ctatgggcca gccattgttc
     8041 taatgttaac tgtggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8101 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8161 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8221 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8281 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8341 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     8401 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     8461 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     8581 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8641 actaattttt tttatttatg cagaggccga ggccggatcc cttgagtggc tttcatcctg
     8701 gagcagactt tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc
     8761 tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg
     8821 gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag
     8881 agggcattag caatagtgtt tataaggccc ccttgttaat tcttgaagac gaaagggcct
     8941 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     9001 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9061 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     9181 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     9241 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     9301 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     9361 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9421 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9481 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9541 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9601 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9661 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9721 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9781 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9841 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9901 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9961 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    10021 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    10081 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10141 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10201 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10261 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
    10321 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10381 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10441 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10501 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
    10561 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10621 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10681 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10741 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggcctttttg
    10801 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
    10861 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcg
//
