LOCUS       sequence-32319-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pJFRC-MUH
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(260..280)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          410..475
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(590..828)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(622..643)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    859..953
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
       61 aacctctaca aatgtggtat ggctgattat gatctctagt caaggcacta tacatcaaat
      121 atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg taaatagcaa
      181 agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc aattctgaag
      241 gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa tgttgagagt
      301 cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg ttttcctcat
      361 taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac
      421 aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata tttaccttag
      481 agctttaaat ctctgtaggt agtttgtcca attatgtcac accacagaag taaggttcct
      541 tcacaaagat cctctagagg taccctcgag ccgcggccgc agatctattc agagttctct
      601 tcttgtattc aataattact tcttggcaga tttcagtagt tgcagttgat ttacttggtt
      661 gctggttact tttaattgat tcactttaac ttgcacttta ctgcagattg tttagcttgt
      721 tcagctgcgc ttgtttattt gcttagcttt cgcttagcga cgtgttcact ttgcttgttt
      781 gaattgaatt gtcgctccgt agacgaagcg cctctattta tactccggcg ctcgacgtcg
      841 ctagcgctag agtctccgct cggaggacag tactccgctc ggaggacagt actccgctcg
      901 gaggacagta ctccgctcgg aggacagtac tccgctcgga ggacagtact ccgacctgca
      961 ggcaggccct a
//
