LOCUS       sequence-323196-       11003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s)
            driven by zebrafish foxj1a promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Zhang J, Chandrasekaran G, Li W, Kim DY, Jeong IY, Lee SH, Liang T, 
            Bae JY, Choi I, Kang H, Maeng JS, Kim MK, Lee T, Park SW, Kim MJ, 
            Kim HS, Ro H, Bae YC, Park HC, Choi EY, Choi SY
  TITLE     Wnt-PLC-IP3-Connexin-Ca(2+) axis maintains ependymal motile cilia in
            zebrafish spinal cord.
  JOURNAL   Nat Commun. 2020 Apr 20;11(1):1860. doi: 10.1038/s41467-020-15248-2.
  PUBMED    32312952
REFERENCE   2  (bases 1 to 11003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-20"; volume: "11(1):1860. doi"; pages: " 
            10.1038/s41467-020-15248-2"
COMMENT     Sequence Label: pSYC-437
FEATURES             Location/Qualifiers
     source          1..11003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6296..6320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6323..6331,6332..6349,6350..6352,6353..6385,6386..6388,
                     6389..6412,6413..6439,6440..6496,6497..6502,6503..6772,
                     6773..6790,6791..7225,7226..7231,7232..7675)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      6419..6428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     6709..6730
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6836..6857)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7097..7116)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(7677..7701)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7703..7837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7900..7920)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7928..7944)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7960..8094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8533..8720,8721..8732)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8766..8782)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8766..8782)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8819..8839)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8819..8837)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8858..8874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8871..8893)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8882..8898
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8906..8912,8913..8930,8931..8936))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8951..8972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9089..9106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9260..9848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9340..9359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10019..10810,10811..10879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10642..10661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10880..10984)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttgtag ctgtatccca tctagacttt
     1141 atctggtgta aaaatatgaa ctattgttga aaagagtcac agaaactatg ctatactgtg
     1201 agtatccgca gctgtatccc gcattgactt catatgtgtg tgtgtgtgtt tgagaagctc
     1261 aaacctgctc gctcccgatc accataacaa ccggccggcc aatgaaagaa cgggactctg
     1321 tttacagggg ggtgtagcgt cgccggggtg accggacaaa cgtcctgcgg caggtattaa
     1381 acggggcccg gagcctctgc tgccccggag accttaaacc gagtcccgta gacgagcaga
     1441 cactccaccg ctggaagaaa acggataaca tttctcattt tcccctcaga aaaccgttgg
     1501 atgttatttc cctgccattc aagaccgcta tcgaggaagg acaggtgagt tgggctcagt
     1561 ttttcacaac gcgagtgtat tttatattag aaatgtgtgt ttataatgat aatataacgt
     1621 tagttatgta aggttagtta acatttattt atcctttgtt tagatttcat ttgacgcaaa
     1681 tacacccaca gagacatttg acaagttcac atttggcatt tatataacgt taaaactacc
     1741 attaatctaa ataaacaacc agtctgctga ccatcaacta ccactaaaga gaggaggatt
     1801 ttaactactt aactgcttga tggataaatg gtcatttttc ataatttaaa gattaaatac
     1861 tagtttttga agcattaaat atattcacat gtaacactgc ttcatttttg tattagaagg
     1921 taacacttaa tgcaactcag tgtaaaaata gtatagattt tcatctttaa aatgctcgtt
     1981 taacaatagt ataaatacaa gaacgggatt tgaagcatta aacatgtaat actgctttat
     2041 tttttattaa aaggtgccct gctttacttg gtttctggtt aagaaatgca cactatcata
     2101 taagtttccc atgctttaga aaggacagca ctgaaatgtc atttagggta aaaaatagta
     2161 ggtaatatta ataatatttt agattttcta aacaattttc gttttttaaa tgtctgttta
     2221 tagcaataat attatgtagc ttaaatacga gaacaggttt tgtagcatta aagacagtaa
     2281 aatttaataa agcttatttt ttcattagat tgtgtcctgt ctgactttat tttaggtcaa
     2341 aaaatgtaca ctatcatata agtttcccac gctttagaaa gggacagcac tgaaatgtca
     2401 ttaattgtaa aaatagtagc taatattaat aatattttag attttcttaa caattttcat
     2461 tttaaaaaat gtgtgtttat agcaataata ttatagcttc aatacgagaa caggttttga
     2521 agcattaaac atgtaataat gctttaattt taaaagaagt cgtcctgctt aacttggttt
     2581 ctggtttaga aatgcacact atcatatttg ttttgcatgt tttagaaagg acagcactga
     2641 attgtcattc attgtaaaaa aaaaaaaaat agtagctaat attaataata ttttagattt
     2701 tcttaacaat tttaattttt aaaatgtgtg tttatagcaa taatattaca tagcttaaat
     2761 atgagaacat gttctgaagc attaaagatg ttaacattta acactgctta tgttttcatt
     2821 agatggtgtc ctgtttgact taattttagg taaaaaaaaa tgcacactag catgtaagtt
     2881 tcccacgctt tagaaaagcc agcactgaaa tgtcattcag tgtaaataaa atagtagcta
     2941 atatcaataa tattatattt tcttaacaat tttcattttt aaaatgtgcg tttataacaa
     3001 taatattaaa taaaagaaca aattttgaag catataacaa tgtttaaatg taatactgct
     3061 ttgattttca ttagatggtg tcctgtttga cttgatttca ggtcaaaaaa tgcacactat
     3121 catatttgtt tctcgtgttt tagaaagaac agccactaaa atatcattca gtgtaaaaaa
     3181 aaagtaggta ataataataa gcttcattta ttttttttaa aagcagctcc tcggagtgct
     3241 gaatatgatg ccaacaaacc actaaataat tcaaaatcaa caagcaaagg caagttttaa
     3301 attaaaaaag ctgtcttcaa atgaataaaa cgatcatcaa tctgagttgt aaaaacaatt
     3361 tacatgctaa aataaatctt cagtcacgtt tagagcaaga ataattaaaa aagatgccat
     3421 ttaatagtca gtttgtcctc aaattgtcat tttaatttat tatactttgc cattatcctt
     3481 accttctacc caagaggttt gtgataaata caaaccaaat caaatttgga gtgatttatt
     3541 catttataca tttgaataag aataggtctg atctagcatg tcatttttac atcatttata
     3601 ctgaaagaca accatattat gacattattg tgtactatta ctctttgtcc acatttaata
     3661 tgctttaatt gtgttttgcg agcctccgtc cgggctgcac ctgccgtgct gaggaatgtt
     3721 agtggttggc tctattgtgt gctgtgaggc agttatgaga cgctgtaaac agtgacctga
     3781 gaagtttcca gaaagttctg taacctctat aagccaaata aagcaacatg tctcacaaac
     3841 gttcatcgtt ttgattgtaa taatgtaaca acatgcacct attgaggagc tgcttgggac
     3901 agtaattgtt gtgaaaagcg ctatacaaaa agttaagtaa ctagtaactg taattaaatt
     3961 acttaatcgc tgaaaaggta atgtgaggga ttacttttga tttttaagta atgtaattag
     4021 taacttaaaa agtacttctc atgaacacac atatataaaa cagtcaatca attcagaaaa
     4081 gcgattatat tttttcatta tacaaatata tgtagctatt tttaattgta tttttcaatt
     4141 acatttagca agtttacatt gaataagaaa ttagtccatg aaaagatatg atataaatcc
     4201 agagaagagt aattataaat ataattaaat ataagccatt acgtgatcat aaatataaat
     4261 ataagctatt gagcaaataa gcggctataa atataagcaa ttaaactgat aagcgattat
     4321 aaatataagc aattacaagc gattaagcgg aatagtaatt ataaagataa gtaactataa
     4381 gcgtttataa atataagtaa ttataagcga ttaagtggaa aggcgattta taaatataag
     4441 taaatatata caattagcga ttataattta agtaaatata agcgattaag caattataaa
     4501 tatataagca aatattgatt ataaatatga tataagcgat taagcaatta taaatgtaag
     4561 taaatttatg ttattaagca attataaaaa taagtaagta taaacaaata gtgattttaa
     4621 atacaagtaa atatagtcga ttaagtggat aagcgattaa aaatataagt aaatataagt
     4681 gattaattga ttataaataa ctataaatga ttaagtgcaa aagtgattat aattataagt
     4741 aaatataagc gattataaat ataagtaact ataaacaatt aagtgtttaa gcgattatga
     4801 atataagtaa atataagcgt ttaagcgatt ataaatataa atagaagcga ttaagtaaat
     4861 aagctattat aaatctaagt aaatataagt gattaagcaa ttataaatat atgtaaattt
     4921 aagcgattaa gtggataagc gattataaat ataagtaaat ataagcgatt gtgcaattat
     4981 aaatataagt aaatataagc aattaagcga ttataaatat aagtaactac aatcggttaa
     5041 tcaaaaaagc gattaaatat ataagtaaat ataagagatt ttgcgattat aaatttaagt
     5101 aaatataagc gattataaat ataaataact aaactggatt aatcgaataa gcgattataa
     5161 atataagcaa ataaacgatt tagtggttat aaatataagt aaataaaaga gattaatcga
     5221 ttaaaaattt aagtaaatat aagcgattaa aaatataagt aactacactc gattaatcga
     5281 ctaagcgata ataaatagaa gtaaatataa gcgatttagc aattataaat atatgtaaat
     5341 ataagcgatt aagcgattat aaataaaagt aaatataagc gattataaat ataagttaat
     5401 ataagcgatt aatcgattat aaatataagt aactataagc gattaattga ttataaatat
     5461 aagtaactat aaccaattaa tctattataa atataagtaa taagcgatta attgattcta
     5521 aatataggta actataagcg attaatcgat tataaattta agtaactata agcgattaaa
     5581 cgattataaa tataagtaac tataagcgat taatcgatta taaatataac taataagcga
     5641 ttaattgatt atgcatacaa gtaaccataa gcgattaatc gattataaat ataagtaact
     5701 ataagcgatt aatcgattat aaatataact aataagcgat taattgatta tacatacaag
     5761 taactataag cgattaatcg attataaata taagtaacta taagcgatta atcgtttata
     5821 aatataagta aatatgagcg attaagcgga taagtgatta taaatataag taaatataaa
     5881 cgattataaa tataagtaac tataagcgag taatcgacta agcgattata aatagaagta
     5941 aatataagag atttagcgat tatagatgta agtaaatata agaaaattag atggataatc
     6001 gattataaat aaaagtaaat ataagcgatt gggggccgtg ggttatcgtt ttttttaatg
     6061 accttaaaac aggatcgtcg ttatgaatgt atttaaactt tttatacccc ttgtttctag
     6121 tgttgtcgag ggaaaatctc agttttgagg gataatatcc cttcccctta gccctacgcc
     6181 ttcaagatga agagaattgg gacaccccta gcccttcaca tgaacactaa ggggtaaggg
     6241 gtagaactgg gattggacct ttgactgatc taaatgtttc tgcctgttag gatttgcaag
     6301 tttgtacaaa aaagcaggct ccatgggttc tcatcatcat catcatcatg gtatggctag
     6361 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatctcgc
     6421 caccatggtc gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat
     6481 aggtcggctg agctcactcg agaacgtcta tatcaaggcc gacaagcaga agaacggcat
     6541 caaggcgaac ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
     6601 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     6661 gagcgtgcag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
     6721 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
     6781 cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     6841 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
     6901 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     6961 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     7021 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg
     7081 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     7141 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     7201 cctggggcac aagctggagt acaacctgcc ggaccaactg actgaagagc agatcgcaga
     7261 atttaaagag gctttctccc tatttgacaa ggacggggat gggacaataa caaccaagga
     7321 gctggggacg gtgatgcggt ctctggggca gaaccccaca gaagcagagc tgcaggacat
     7381 gatcaatgaa gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat
     7441 gatggcaaga aaaatgaaat acagggacac ggaagaagaa attagagaag cgttcggtgt
     7501 gtttgataag gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa
     7561 ccttggagag aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga
     7621 tggggatggt caggtaaact acgaagagtt tgtacaaatg atgacagcga agtgacaccc
     7681 agctttcttg tacaaagtgg gggatccaga catgataaga tacattgatg agtttggaca
     7741 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     7801 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     7861 tatgtttcag gttcaggggg aggtgtggga ggttttttcc aactttatta tacatagttg
     7921 ataattcact ggccgtcgtt ttacggtacc atcgatgatg atccagacat gataagatac
     7981 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     8041 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     8101 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     8161 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca gatctgcgaa
     8221 gatacggcca cgggtgctct tgatcctgtg gctgattttg gactgtgctg ctcgcagctg
     8281 ctgatgaatc acatacttcc tccattttct tccactgatt gactgttata atttccctaa
     8341 tttccaggtc aaggtgctgt gcattgtggt aatagatgtg acatgacgtc acttccaaag
     8401 gaccaatgaa catgtctgac caatttcata taatgtgaaa acgattttca taggcagaat
     8461 aaataacatt taaattaaac tgggcatcag cgcaattcaa ttggtttggt aatagcaagg
     8521 gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa taaaattgta
     8581 aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta aaagtattga
     8641 tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag tacagtaatc
     8701 aagtaaaatt actcaagtac tttacacctc tggttcttga ccccctacct tcagcaagcc
     8761 cagcagatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc
     8821 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8881 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8941 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9001 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9061 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9241 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9421 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9661 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9901 cacgttaagg cattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10081 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10141 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10201 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10261 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10321 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10381 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10441 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10501 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10561 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10621 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10681 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10741 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10801 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10861 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10981 cgcgcacatt tccccgaaaa gtg
//
