LOCUS       sequence-32321-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pJFRC2-10XUAS-IVS-mCD8::GFP
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..690)
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     intron          711..777
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(824..845)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 gtcttctcct ttactcatgg atccgcggct gtggtagcag atgagagtga tgatcaagga
       61 cagcagaagg gccacgcaga ttccggccaa gggtgcccag atgtaaatat cacaggcgaa
      121 gtccaatccg gtccccttca ctgagccacg gggccgacaa tcttctggtc tctggggctg
      181 agatgtcccg gtagggtgca caggtgaggg agttcgcagc actggcttgg tagtagtaga
      241 gttcactttc tgaaggactg gcacgacaga actgaagtac atcaccgagt tgctgatgac
      301 tgagcagaaa tagtagcctt cgttttcctt gctgaacttg ttcagggtga gaacgtactt
      361 attattcgtg tccctcatgg cagaaaacag tttcgacgaa ttcagcttct cgtcccacgt
      421 tatcttgttg tgggatgaag ccatatagac aacgaaggtg ggctggggga gtttggagct
      481 ggagttctgg aagagccaag agcatccttg cgaaacggac cccaacactt cacataccag
      541 gtccaccttc tgaccaagtt cggcgtccat tttctttgga aagattcgga gttcgggtgc
      601 ctgtggctta gcttctccac tccccaggat aatcgactca cccagcagca gcaggttcag
      661 cgacagaaag cgggtcaacg gtgaggccat tttgctcgag ccgcggccgc ctgaagtaaa
      721 ggataagaat tagggttagt cgtttcggtg tgcctaggca tcagtggttg aacctacctt
      781 ttagatctat tcagagttct cttcttgtat tcaataatta cttcttggca gatttcagta
      841 gttgcagttg atttacttgg ttgctggtta cttttaattg attcacttta acttggcact
      901 tttactgcag attg
//
