LOCUS       sequence-32323-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pJFRC2-INS
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..674)
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     intron          695..761
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(808..829)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 atggatccgc ggctgtggta gcagatgaga gtgatgatca aggacagcag aagggccacg
       61 cagattccgg ccaagggtgc ccagatgtaa atatcacagg cgaagtccaa tccggtcccc
      121 ttcactgagc cacggggccg acaatcttct ggtctctggg gctgagatgt cccggtaggg
      181 tgcacaggtg agggagttcg cagcactggc ttggtagtag tagagttcac tttctgaagg
      241 actggcacga cagaactgaa gtacatcacc gagttgctga tgactgagca gaaatagtag
      301 ccttcgtttt ccttgctgaa cttgttcagg gtgagaacgt acttattatt cgtgtccctc
      361 atggcagaaa acagtttcga cgaattcagc ttctcgtccc acgttatctt gttgtgggat
      421 gaagccatat agacaacgaa ggtgggctgg gggagtttgg agctggagtt ctggaagagc
      481 caagagcatc cttgcgaaac ggaccccaac acttcacata ccaggtccac cttctgacca
      541 agttcggcgt ccattttctt tggaaagatt cggagttcgg gtgcctgtgg cttagcttct
      601 ccactcccca ggataatcga ctcacccagc agcagcaggt tcagcgacag aaagcgggtc
      661 aacggtgagg ccattttgct cgagccgcgg ccgcctgaag taaaggataa gaattagggt
      721 tagtcgtttc ggtgtgccta ggcatcagtg gttgaaccta ccttttagat ctattcagag
      781 ttctcttctt gtattcaata attacttctt ggcagatttc agtagttgca gttgatttac
      841 ttggttgctg gttactttta attgattcac tttaacttgc actttactgc
//
