LOCUS       sequence-323244-        3142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fusion grammar bacterial CP6 promoter, assembles with pHSP70B-TACT .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pCP6-Dros
FEATURES             Location/Qualifiers
     source          1..3142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..36
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      70..79
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(300..318)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(301..318)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(336..352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(336..352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(349..371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    360..376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(384..390,391..408,409..414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    429..450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(567..584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(738..1326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(818..837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1497..2288,2289..2357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2120..2139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2358..2462)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2540..2995)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2677..2698)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2889..2908
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3121..3142,1)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3135..3142,1..10)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(3136..3142,1..10)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggcccga cgtcgcatgc
       61 tcccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgtactcgaa
      121 tccatgtggg agtttattct tgacacagat atttatgata taataactga gtaagcttaa
      181 cataaggagg aaaacaaaat gcgagaccac tcatcgccca atcactagtg aattcgcggc
      241 cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt
      301 ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      361 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      421 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1141 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1621 gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag
     1681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1801 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2101 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2161 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2221 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2281 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2341 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2401 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2461 cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag
     2521 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     2581 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     2641 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     2701 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgctggccca
     2761 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     2821 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     2881 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     2941 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt
     3001 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     3061 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     3121 cccagtcacg acgttgtaaa ac
//
