LOCUS       sequence-323283-        2851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QF activation domain, requires assembly with TL2-TL4 linker and TL1 
            DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pQFAD
FEATURES             Location/Qualifiers
     source          1..2851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(653..671)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(654..671)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(687..703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(687..703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(818..1406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(898..917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1521..1623
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1624..2283
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1690..1709)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2396..2824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2483..2502)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2693..2714
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcgaagctt actgctcgta
      121 agtgttgatg tcggagaagt tgcagccggg aacgaaaccc aggttggtca tgaactgcgg
      181 atcgacgtca acggcgtcag tgtactcgat ggaagccaaa tcgtccacca tggcatcgta
      241 gtcaacggtg gagtatccga agttagccat gggcaccagg ttggagtggt agggcgggtg
      301 gttggtggtg gagtcgacgt aggggggcgg gggacggggt ggagctccgc cgggagccag
      361 ggcgacggag gcctcgttga ggtgggtggc tggcgggggg taagtggaca agatgccgtg
      421 ggtattgacg gaaccgtagt cgttggtctg ggtgatggcg tccagggcgg cgtcggagtt
      481 gtagggggtg gggtttggga tgccgttgaa gcgatcgttg acggggttga tggaggtgga
      541 cagcggctgc tccatgacgt gtggcataat cgaagcggtg gcggtggcag tgggtggcag
      601 ctccagctgg cgcgagcgag accactcatc gcccaatcac tagtgaattc gttctatagt
      661 gtcacctaaa taccatagag ccgttggtca tagctgtttc ctgtccaggc atcatgacac
      721 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      781 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      841 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      901 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      961 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1021 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1081 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1141 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1201 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1261 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1321 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1381 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1441 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1501 tcacctagat ccttttgtga tgatcggcac gtaagaggtt ccaactttca ccataatgaa
     1561 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
     1621 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     1681 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1741 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1801 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     1861 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1921 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1981 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     2041 gagaatatgt ttttcgtgtc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     2101 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     2161 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     2221 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     2281 taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt
     2341 gataataagc ggatgaatgg cagaaattcg atgataagct gtcaaacatg atgttacgcg
     2401 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2461 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2521 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2581 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccagcg
     2641 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2701 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2761 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2821 aattttaaca aaatattaac gcttacaatt t
//
