LOCUS       sequence-323285-        5101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QF inhibitor coding sequence (CDS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pQS
FEATURES             Location/Qualifiers
     source          1..5101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      211..220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2903..2921)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2904..2921)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2937..2953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2937..2953)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(3068..3656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3148..3167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3771..3873
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3874..4533
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3940..3959)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      4646..5074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4733..4752)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4943..4964
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcgaatgaa caccatcccc
      121 gctcgtcacg ttggcgatgt cgctgctcgc gatcccctcc cgttgccaca catctccagc
      181 agcgtggctt ccggcatgaa gcgttccttc gccaccatgg ccatgctcta caacgacacc
      241 ggaaattcca acgatgtggg cgcccacgcc cgccgcccac cccgtaccct gtcgaactcg
      301 cgttccacct ccgcccaccg cgttcccctc ggctcctggt cggcccccaa ctcgcccccc
      361 cgtcgtgcct tgccccacca ccccatcacc gctagcttcg atcccgatgc ctccatcgtg
      421 atcgctggta tccgtggcgc tggcaagtcc actttggcca tcatggcctc caccgccatg
      481 aagcgcaaga tcgtcgatct ggagtccgag ttccaccacc tgaccggcct gagctcctcc
      541 agctacaaga aaacccatgg ccccgtcgac tacggccgtc gtcagatcgc tatcctgcag
      601 aacattctga atctgcaccg cacccgcgcc atcctcgtgt gctcctggct ggagcgcgac
      661 gtccaggcca tgctgcagga tttcagcgtg tccaaccccg ttatctacgt gttgcgcgat
      721 gccaaggcta ttgaggccca cctgaaggga tacgacaaga gcaaagtcgg taccctgctg
      781 gatgccacct cgaccgtgct gcgccgctgc acccgtttcg agttcttcaa cgtttcggag
      841 gagaatctgg atacccacag cgcctccacc tccccgcccg ccgtcccaga tcagcgccac
      901 accgccccct acctcaccct caagcgcgcc gagcgccact tcctgaagtt cctgtccctg
      961 atcctgccaa agggcaccat ccccttcgtg gagtccgcct tcccgctggc cagcgtgccc
     1021 gtggagcagc gtcgtttcac ctacgccctg gccctccccg tgtccgccct gctggacaag
     1081 ggcgtcgaca tccaggagct ggacgtcggt gtcgatgcca tcgagatcat cgtcgatgat
     1141 ctggccacct ccgagtccgg acccacctcg ccgctcggac tggccccaca ccgtgcctcc
     1201 gagatctccc gcgttgtggg cgagatccgc cgtgacaccg tcatccccat cattctccac
     1261 gtcgtgttcc ccgaacgtgc cctgtatgag gaggctctgc tcgctctgta catgacctac
     1321 ctgaatcacg ccctgcgttt ggcccccgat tacctgaccg tggacctggg cctcgactcg
     1381 ggcctgttgg gccagttgac taccgtgcag ggcactacca aggtgatcgg taacaagcag
     1441 ctggccgaag tcaactcgcc acgttggggc gatccctcgt ggttgcaggc ttacgagaag
     1501 gctcagaaca ccggctgcga tctcgtccgc ctgacccgcc ccgctagcaa cccacgtgat
     1561 aacaccgaca ttcgccagtt ccacgtggct gttgaagcag ttggaggccc ccgtctgccc
     1621 ttcatcgcct acaataccgg acgcctcggc cgcacctcca tgtgcttcaa cgagattctg
     1681 acccccgtta ctcccgtgcc cactaaggag gatgccatcg gactgcgcaa ccccgcccac
     1741 cgttacctgc aaccccccct gaccgctctg gaggccaccc aggccttgta ctccgccttc
     1801 gttcacgatc ccatgaagct ctacgtgttc ggtgccaacg tcggatactc cttgagcccc
     1861 gccatgcata acgccgctct gaaggcctgc ggaattcccc accactacaa gcccctgtcc
     1921 accgccaaca tcggaaccct gcgtgaggtt atcagcgacc cacaattcgc cggcgcttcg
     1981 gtcggtctgc ccttcaaggt tgagatcatc tccctgaccc actcgctgtc gcgtcacgct
     2041 aaggccatcg gagccgtcaa caccctcatc ccagtccgcc acttgactgc cgacggtggt
     2101 attcccgacg aggtgtcgat gttcaacaac atcagccagg ccggtgccgt ccgcgctctg
     2161 tatggcgaga acaccgactg gatcggcatc cgcgcctgcc tgcgtcgtgg cctgtccccc
     2221 gccaacgccg tgcgctcgac ctccaccgga ctggtcattg gcgctggcgg catggcccgt
     2281 gccgccgtgt acgccatgct gcagctgggt gtcaagaaga tcctgatctt caaccgcacc
     2341 ttcgccaatg ccgagaagtt ggttctccac ttcgaaaacc tgttggtccg tgacgctctg
     2401 cccctgctgt ccaccggacc ccgttcccac gacaacacgt gcttccacat tatccgctcc
     2461 cgtgatgacc ccctgcccga gaatttcaag aaccccacca tgatcgtgtc ctgcatcccc
     2521 acccacaccg tcgacaacac ccccgacccc gagttcaccg tgccactcca ttggctcgat
     2581 aatcccaccg gcggaatcgt cctggagctg gattacaagt gcctgacctc ccccctgctg
     2641 gagcagaccc gccgtgaggc ccaccgcggt tgggtggcca tggatggcct ggatctgctg
     2701 ccggagcagg gcttcgccca gttcgagttg ttcaccggtc gccgcgctcc acgccgtctg
     2761 atgcgtcgcg aagtcctgcg cgcctatccc gatgaccagg ccaagagcca caccgcccag
     2821 ctccagccac gcctgaacgg aattgccacc cagatttcct aagcttcgag accactcatc
     2881 gcccaatcac tagtgaattc gttctatagt gtcacctaaa taccatagag ccgttggtca
     2941 tagctgtttc ctgtccaggc atcatgacac ggttatccac agaatcaggg gataacgcag
     3001 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3061 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3121 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3181 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3241 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3301 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3361 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3421 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3481 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3541 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3601 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3661 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3721 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgtga tgatcggcac
     3781 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     3841 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     3901 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     3961 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     4021 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     4081 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     4141 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     4201 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     4261 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtgtc agccaatccc
     4321 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     4381 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     4441 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     4501 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     4561 taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     4621 atgataagct gtcaaacatg atgttacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4681 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4741 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4801 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4861 tagggtgatg gttcacgtag tgggccagcg ccctgataga cggtttttcg ccctttgacg
     4921 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4981 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5041 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5101 t
//
